PMID- 30572028
OWN - NLM
STAT- MEDLINE
DCOM- 20191230
LR  - 20191230
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 68
DP  - 2019 Mar
TI  - Co-infection status of classical swine fever virus (CSFV), porcine reproductive 
      and respiratory syndrome virus (PRRSV) and porcine circoviruses (PCV2 and PCV3) 
      in eight regions of China from 2016 to 2018.
PG  - 127-135
LID - S1567-1348(18)30737-8 [pii]
LID - 10.1016/j.meegid.2018.12.011 [doi]
AB  - Classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome 
      virus (PRRSV) and porcine circoviruses (PCV2 and PCV3) are economically important 
      swine viruses that cause reproductive failure and/or respiratory symptoms in 
      pigs. However, the co-infection status of these viruses in Chinese swine herds is 
      not well clarified. In this study, we evaluated the co-infection of these four 
      viruses in 159 pigs collected from 63 herds in eight regions of China from 2016 
      to 2018. CSFV, PRRSV, PCV2 and PCV3 were detected in 14, 56, 43 and 4 of the 
      pigs, respectively. The percentage of singular infections was 32.71%, while the 
      percentages of dual infections and multiple infections were 15.72% and 3.15%, 
      respectively. The E2 of CSFV, ORF5 of PRRSV, ORF2s of PCV2 and PCV3 from all 
      positive samples were determined and used for phylogenetic analyses. E2-based 
      phylogenetic tree showed that all 14 CSFVs identified in this study belong to 
      2.1b subtype. ORF5-based phylogenetic tree showed that PRRSV2 is predominant in 
      China while PRRSV1 can also be detected. In addition, 35, 16, 4 and 1 of our 
      PRRSVs are clustered with highly pathogenic PRRSV2, NADC30-like PRRSV2, classical 
      PRRSV2 and PRRSV1, respectively. ORF2-based phylogenetic trees showed that our 
      PCVs are grouped with 2 PCV2 subtypes (PCV2d and PCV2b) and 3 PCV3 subtypes 
      (PCV3a, PCV3b and PCV3c), respectively. Our results provide the latest 
      co-infection status and the diversity of four important swine viruses in Chinese 
      swine herds, which is beneficial for understanding the epidemiology of these 
      viruses.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Chen, Nanhua
AU  - Chen N
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Comparative Medicine 
      Research Institute, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; 
      Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China. Electronic address: 
      chnhlh@126.com.
FAU - Huang, Yucheng
AU  - Huang Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
FAU - Ye, Mengxue
AU  - Ye M
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
FAU - Li, Shuai
AU  - Li S
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
FAU - Xiao, Yanzhao
AU  - Xiao Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
FAU - Cui, Bailei
AU  - Cui B
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
FAU - Zhu, Jianzhong
AU  - Zhu J
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Comparative Medicine 
      Research Institute, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; 
      Joint International Research Laboratory of Agriculture and Agri-Product Safety, 
      Yangzhou University, Yangzhou, Jiangsu 225009, PR China. Electronic address: 
      jzzhu@yzu.edu.cn.
LA  - eng
PT  - Historical Article
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181217
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
SB  - IM
MH  - Animals
MH  - China/epidemiology
MH  - Circoviridae Infections/*veterinary
MH  - *Circovirus/classification/genetics
MH  - Classical Swine Fever/*epidemiology/history/virology
MH  - *Classical Swine Fever Virus/classification/genetics
MH  - *Coinfection
MH  - History, 21st Century
MH  - Multilocus Sequence Typing
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology/history/virology
MH  - *Porcine respiratory and reproductive syndrome virus/classification/genetics
MH  - Swine
OTO - NOTNLM
OT  - Classical swine fever virus
OT  - Co-infection
OT  - Evolution
OT  - Porcine circoviruses
OT  - Porcine reproductive and respiratory syndrome virus
EDAT- 2018/12/21 06:00
MHDA- 2019/12/31 06:00
CRDT- 2018/12/21 06:00
PHST- 2018/09/26 00:00 [received]
PHST- 2018/11/19 00:00 [revised]
PHST- 2018/12/06 00:00 [accepted]
PHST- 2018/12/21 06:00 [pubmed]
PHST- 2019/12/31 06:00 [medline]
PHST- 2018/12/21 06:00 [entrez]
AID - S1567-1348(18)30737-8 [pii]
AID - 10.1016/j.meegid.2018.12.011 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2019 Mar;68:127-135. doi: 10.1016/j.meegid.2018.12.011. Epub 
      2018 Dec 17.