PMID- 30572664
OWN - NLM
STAT- MEDLINE
DCOM- 20190415
LR  - 20201209
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 10
IP  - 12
DP  - 2018 Dec 19
TI  - Global Interactomics Connect Nuclear Mitotic Apparatus Protein NUMA1 to Influenza 
      Virus Maturation.
LID - 10.3390/v10120731 [doi]
LID - 731
AB  - Influenza A virus (IAV) infections remain a major human health threat. IAV has 
      enormous genetic plasticity and can rapidly escape virus-targeted anti-viral 
      strategies. Thus, there is increasing interest to identify host proteins and 
      processes the virus requires for replication and maturation. The IAV 
      non-structural protein 1 (NS1) is a critical multifunctional protein that is 
      expressed to high levels in infected cells. Host proteins that interact with NS1 
      may serve as ideal targets for attenuating IAV replication. We previously 
      developed and characterized broadly cross-reactive anti-NS1 monoclonal 
      antibodies. For the current study, we used these mAbs to co-immunoprecipitate 
      native IAV NS1 and interacting host proteins; 183 proteins were consistently 
      identified in this NS1 interactome study, 124 of which have not been previously 
      reported. RNAi screens identified 11 NS1-interacting host factors as vital for 
      IAV replication. Knocking down one of these, nuclear mitotic apparatus protein 1 
      (NUMA1), dramatically reduced IAV replication. IAV genomic transcription and 
      translation were not inhibited but transport of viral structural proteins to the 
      cell membrane was hindered during maturation steps in NUMA1 knockdown (KD) cells.
FAU - Rahim, Md Niaz
AU  - Rahim MN
AUID- ORCID: 0000-0003-2042-134X
AD  - Department of Medical Microbiology, Max Rady College of Medicine, University of 
      Manitoba, Winnipeg, MB R3E 0J6, Canada. mdniaz.rahim@canada.ca.
AD  - Manitoba Centre for Proteomics & Systems Biology, Room 799, 715 McDermot Avenue, 
      Winnipeg, MB R3E 3P4, Canada. mdniaz.rahim@canada.ca.
FAU - Klewes, Ludger
AU  - Klewes L
AD  - Department of Physiology and Pathophysiology, Max Rady College of Medicine, 
      University of Manitoba, Winnipeg, MB R3E 0J6, Canada. Ludger.Klewes@umanitoba.ca.
AD  - Genomic Centre for Cancer Research and Diagnosis, Research Institute in Oncology 
      and Hematology, ON6026-675 McDermot Avenue, Winnipeg, MB R3E 0V9, Canada. 
      Ludger.Klewes@umanitoba.ca.
FAU - Zahedi-Amiri, Ali
AU  - Zahedi-Amiri A
AD  - Department of Medical Microbiology, Max Rady College of Medicine, University of 
      Manitoba, Winnipeg, MB R3E 0J6, Canada. zahediaa@myumanitoba.ca.
AD  - Manitoba Centre for Proteomics & Systems Biology, Room 799, 715 McDermot Avenue, 
      Winnipeg, MB R3E 3P4, Canada. zahediaa@myumanitoba.ca.
FAU - Mai, Sabine
AU  - Mai S
AUID- ORCID: 0000-0002-5797-2201
AD  - Department of Physiology and Pathophysiology, Max Rady College of Medicine, 
      University of Manitoba, Winnipeg, MB R3E 0J6, Canada. Sabine.Mai@umanitoba.ca.
AD  - Genomic Centre for Cancer Research and Diagnosis, Research Institute in Oncology 
      and Hematology, ON6026-675 McDermot Avenue, Winnipeg, MB R3E 0V9, Canada. 
      Sabine.Mai@umanitoba.ca.
FAU - Coombs, Kevin M
AU  - Coombs KM
AD  - Department of Medical Microbiology, Max Rady College of Medicine, University of 
      Manitoba, Winnipeg, MB R3E 0J6, Canada. Kevin.Coombs@umanitoba.ca.
AD  - Manitoba Centre for Proteomics & Systems Biology, Room 799, 715 McDermot Avenue, 
      Winnipeg, MB R3E 3P4, Canada. Kevin.Coombs@umanitoba.ca.
AD  - Children's Hospital Research Institute of Manitoba, Room 513, John Buhler 
      Research Centre, 715 McDermot Avenue, Winnipeg, MB R3E 3P4, Canada. 
      Kevin.Coombs@umanitoba.ca.
LA  - eng
GR  - MOP-106713/CIHR/Canada
GR  - PAN-83159/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181219
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Antigens, Nuclear)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (NUMA1 protein, human)
RN  - 0 (Nuclear Matrix-Associated Proteins)
RN  - 0 (Viral Nonstructural Proteins)
SB  - IM
MH  - A549 Cells
MH  - Antigens, Nuclear/genetics/*metabolism
MH  - Cell Cycle Proteins
MH  - Cell Line
MH  - Gene Knockdown Techniques
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Immunoprecipitation
MH  - Influenza A virus/genetics/*physiology
MH  - Nuclear Matrix-Associated Proteins/genetics/*metabolism
MH  - RNA Interference
MH  - Viral Nonstructural Proteins/genetics/*metabolism
MH  - *Virus Assembly
MH  - Virus Replication
PMC - PMC6316800
OTO - NOTNLM
OT  - Influenza A virus (IAV)
OT  - Nuclear mitotic apparatus protein 1 (NUMA1)
OT  - Viral replication
OT  - immunoprecipitation (IP), Western blotting
OT  - knockdown (KD)
OT  - monoclonal antibodies (mAbs)
OT  - siRNA
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/12/24 06:00
MHDA- 2019/04/16 06:00
PMCR- 2018/12/01
CRDT- 2018/12/22 06:00
PHST- 2018/10/01 00:00 [received]
PHST- 2018/12/18 00:00 [revised]
PHST- 2018/12/18 00:00 [accepted]
PHST- 2018/12/22 06:00 [entrez]
PHST- 2018/12/24 06:00 [pubmed]
PHST- 2019/04/16 06:00 [medline]
PHST- 2018/12/01 00:00 [pmc-release]
AID - v10120731 [pii]
AID - viruses-10-00731 [pii]
AID - 10.3390/v10120731 [doi]
PST - epublish
SO  - Viruses. 2018 Dec 19;10(12):731. doi: 10.3390/v10120731.