PMID- 30575053
OWN - NLM
STAT- MEDLINE
DCOM- 20200817
LR  - 20231213
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 39
IP  - 5
DP  - 2019 May
TI  - Regulation of NRF2, AP-1 and NF-kappaB by cigarette smoke exposure in 
      three-dimensional human bronchial epithelial cells.
PG  - 717-725
LID - 10.1002/jat.3761 [doi]
AB  - Cigarette smoke (CS) is a complex mixture of chemicals and interacts with various 
      physiological processes. We previously reported that nuclear factor erythroid 
      2-related factor 2 (NRF2) was the most sensitive transcription factor to aqueous 
      CS extract (AqCSE) exposure in monolayer cultured human bronchial epithelial cell 
      lines. Recently, in vitro three-dimensional (3D) culture models have been used to 
      supplement pharmacological and toxicological assessments. Bronchial epithelium 
      models in particular are useful for the evaluation of substances that directly 
      contact the respiratory tract, such as CS. In the present study, we used 
      3D-cultured human bronchial epithelial cells (HBECs) to assess activation of 
      transcription factors and relevant gene expression in response to AqCSE, 
      primarily focusing on NRF2 and nuclear factor-kappa B (NF-kappaB) pathways. The 
      3D-cultured HBECs exposed to AqCSE showed expression of NRF2 and its nuclear 
      translocation in addition to upregulation of genes related to oxidative stress. 
      Our results suggest that the NRF2 pathway was the dominant pathway when 
      3D-cultured HBECs were exposed to AqCSE at a low dose, supporting our previous 
      findings that NRF2 was the most sensitive transcription factor in response to 
      AqCSE. Expression and nuclear translocation of NF-kappaB were not increased, although 
      proinflammatory genes were upregulated. However, another inflammation-related 
      transcription factor, activation protein 1, was induced by AqCSE. Gene 
      classification analysis suggested that induction of the inflammatory response by 
      AqCSE was dependent on NRF2 and activation protein 1 rather than NF-kappaB.
CI  - (c) 2018 John Wiley & Sons, Ltd.
FAU - Sekine, Takashi
AU  - Sekine T
AUID- ORCID: 0000-0003-4513-0768
AD  - Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2, 
      Umegaoka, Aoba-ku, Yohohama, Kanagawa, 227-8512, Japan.
AD  - Laboratory of Cellular Pharmacology, School of Pharmacy, Aichi-Gakuin University, 
      1-100 Kusumoto, Chikusa, Nagoya, 464-8650, Japan.
FAU - Hirata, Tadashi
AU  - Hirata T
AD  - Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2, 
      Umegaoka, Aoba-ku, Yohohama, Kanagawa, 227-8512, Japan.
FAU - Ishikawa, Shinkichi
AU  - Ishikawa S
AD  - Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2, 
      Umegaoka, Aoba-ku, Yohohama, Kanagawa, 227-8512, Japan.
FAU - Ito, Shigeaki
AU  - Ito S
AD  - Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2, 
      Umegaoka, Aoba-ku, Yohohama, Kanagawa, 227-8512, Japan.
FAU - Ishimori, Kanae
AU  - Ishimori K
AD  - Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2, 
      Umegaoka, Aoba-ku, Yohohama, Kanagawa, 227-8512, Japan.
FAU - Matsumura, Kazushi
AU  - Matsumura K
AD  - Scientific Product Assessment Center, R&D Group, Japan Tobacco Inc., 6-2, 
      Umegaoka, Aoba-ku, Yohohama, Kanagawa, 227-8512, Japan.
FAU - Muraki, Katsuhiko
AU  - Muraki K
AD  - Laboratory of Cellular Pharmacology, School of Pharmacy, Aichi-Gakuin University, 
      1-100 Kusumoto, Chikusa, Nagoya, 464-8650, Japan.
LA  - eng
PT  - Journal Article
DEP - 20181221
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Smoke)
RN  - 0 (Transcription Factor AP-1)
SB  - IM
MH  - Adult
MH  - Bronchi/*drug effects/metabolism
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Epithelial Cells/*drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Male
MH  - NF-E2-Related Factor 2/genetics/*metabolism
MH  - NF-kappa B/genetics/*metabolism
MH  - Oxidative Stress/drug effects
MH  - Smoke/*adverse effects
MH  - Smoking/adverse effects
MH  - Nicotiana/*toxicity
MH  - Transcription Factor AP-1/genetics/*metabolism
OTO - NOTNLM
OT  - activator protein 1
OT  - cigarette smoke
OT  - human bronchial epithelial cells
OT  - inflammation
OT  - nuclear factor erythroid 2-related factor 2
OT  - nuclear factor-kappa B
OT  - oxidative stress
OT  - three-dimensional culture
EDAT- 2018/12/24 06:00
MHDA- 2020/08/18 06:00
CRDT- 2018/12/22 06:00
PHST- 2018/10/03 00:00 [received]
PHST- 2018/11/07 00:00 [revised]
PHST- 2018/11/08 00:00 [accepted]
PHST- 2018/12/24 06:00 [pubmed]
PHST- 2020/08/18 06:00 [medline]
PHST- 2018/12/22 06:00 [entrez]
AID - 10.1002/jat.3761 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2019 May;39(5):717-725. doi: 10.1002/jat.3761. Epub 2018 Dec 21.