PMID- 30575914
OWN - NLM
STAT- MEDLINE
DCOM- 20191213
LR  - 20211204
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 22
IP  - 24
DP  - 2018 Dec
TI  - Integrin-linked kinase is overexpressed in laryngeal squamous cell carcinoma and 
      correlates with tumor proliferation, migration and invasion.
PG  - 8740-8748
LID - 16639 [pii]
LID - 10.26355/eurrev_201812_16639 [doi]
AB  - OBJECTIVE: To investigate the role of integrin-linked kinase (ILK) in invasion 
      and metastasis of the laryngeal squamous cell carcinomas (LSCC) and to evaluate 
      the effects of antisense oligonucleotide sequence (ASONs) targeting the ILK gene 
      on the proliferation, epithelial-mesenchymal transition (EMT), migration and 
      invasion of LSCC. PATIENTS AND METHODS: 116 patients who had previously undergone 
      complete resection of the tumor for LSCC were studied retrospectively. The ILK 
      expression level in tumor tissues and adjacent normal tissues were determined by 
      immunohistochemistry. The changes of ILK expression from each group were assessed 
      and correlated to the clinical parameters of the patients. Secondly, ILK 
      antisense oligonucleotide (ILK-ASONS) was used to silence the ILK gene of LSCC 
      cell from Hep-2 cell line. The expression of ILK, epithelial marker E-cadherin 
      and mesenchymal marker Vimentin were evaluated by Western blotting. The 
      proliferation of cells after transfection was measured by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The apoptosis 
      was detected by flow cytometry. The migration and invasion activity of Hep-2 
      cells was detected by Matrigel invasion and cell migration assays. RESULTS: The 
      expression of the ILK protein was significantly associated with tumor 
      differentiation (p=0.046), lymph node metastasis (p=0.020) and pTNM stage 
      (p=0.019). ILK ASONS-transfected cells showed a significant decrease in cell 
      proliferation, cell migration and invasive activity compared to mock-transfected 
      cells. ILK ASONS-transfected cells increased the expression of E-cadherin, 
      whereas the expression of ILK and Vimentin decreased, compared with 
      mock-transfected cells. CONCLUSIONS: The expression of ILK was significantly 
      correlated with differentiation and metastasis of the laryngeal carcinomas. The 
      inhibition of the ILK gene could downregulate the proliferation, migration and 
      invasion of Hep-2 cells. These findings suggest that the ILK gene could be a 
      potential target for the treatment of laryngeal cancer.
FAU - Wu, P-A
AU  - Wu PA
AD  - Department of Otolaryngology, Head and Neck Surgery, the University of Hong 
      Kong-Shenzhen Hospital, Shenzhen, Guangdong, P.R. China. wupa@hku-szh.org.
FAU - Xie, L-L
AU  - Xie LL
FAU - Zhao, D-Y
AU  - Zhao DY
FAU - Li, S-S
AU  - Li SS
FAU - Tang, Q-L
AU  - Tang QL
FAU - Wang, S-H
AU  - Wang SH
FAU - Yang, X-M
AU  - Yang XM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - EC 2.7.1.- (integrin-linked kinase)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Epithelial-Mesenchymal Transition
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*pathology/surgery
MH  - Laryngectomy
MH  - Larynx/pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness/pathology
MH  - *Oncogenes
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/*pathology/surgery
EDAT- 2018/12/24 06:00
MHDA- 2019/12/18 06:00
CRDT- 2018/12/22 06:00
PHST- 2018/12/22 06:00 [entrez]
PHST- 2018/12/24 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
AID - 16639 [pii]
AID - 10.26355/eurrev_201812_16639 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2018 Dec;22(24):8740-8748. doi: 
      10.26355/eurrev_201812_16639.