PMID- 30578621 OWN - NLM STAT- MEDLINE DCOM- 20190617 LR - 20190617 IS - 1476-5829 (Electronic) IS - 1476-5810 (Linking) VI - 17 IP - 2 DP - 2019 Jun TI - Minimal residual disease in lymph nodes after achievement of complete remission predicts time to relapse in dogs with large B-cell lymphoma. PG - 139-146 LID - 10.1111/vco.12453 [doi] AB - Most dogs with large B-cell lymphoma (LBCL) that undergo chemotherapy and achieve clinical complete remission (CR) eventually relapse. However, time to relapse (TTR) is unpredictable. The aims of this prospective study were to assess the influence of post-chemotherapy lymph node (LN) infiltration by large CD21+ cells using flow cytometry (FC) on TTR, and to establish a cut-off value of prognostic significance. Dogs with newly-diagnosed, completely staged LBCL in CR after treatment were enrolled. Minimal residual disease (MRD) analysis by FC was performed on LN aspirates. TTR was calculated between MRD and relapse. Thirty-one dogs were enrolled: 4% had stage V disease, and diffuse large B-cell lymphoma was the most common histotype (74%). Based on LN infiltration at MRD evaluation, three groups were created: (a) acellular samples, (b) 0.5% infiltration. Overall median TTR was 154 days (range, 31-1974): 22 (71%) dogs relapsed during the study period, whereas 9 (29%) dogs did not. The difference among the three groups was significant (P = 0.042 log-rank test): median TTR was not reached for dogs with LN infiltration 0.5%. These results demonstrate that MRD assessment by FC on LN aspirates in dogs with LBCL in clinical CR predicts TTR. LN infiltration by >0.5% large CD21+ cells after treatment is an unfavourable prognostic factor. CI - (c) 2018 John Wiley & Sons Ltd. FAU - Chalfon, Carmit AU - Chalfon C AD - Centro Oncologico Veterinario, Bologna, Italy. FAU - Martini, Valeria AU - Martini V AUID- ORCID: 0000-0002-9882-2867 AD - Department of Veterinary Medicine, University of Milan, Milan, Italy. FAU - Comazzi, Stefano AU - Comazzi S AUID- ORCID: 0000-0002-1567-8055 AD - Department of Veterinary Medicine, University of Milan, Milan, Italy. FAU - Aresu, Luca AU - Aresu L AD - Department of Veterinary Science, University of Turin, Turin, Italy. FAU - Stefanello, Damiano AU - Stefanello D AUID- ORCID: 0000-0003-2726-0366 AD - Department of Veterinary Medicine, University of Milan, Milan, Italy. FAU - Riondato, Fulvio AU - Riondato F AD - Department of Veterinary Science, University of Turin, Turin, Italy. FAU - Ferrari, Roberta AU - Ferrari R AUID- ORCID: 0000-0002-7960-6165 AD - Department of Veterinary Medicine, University of Milan, Milan, Italy. FAU - Marconato, Laura AU - Marconato L AUID- ORCID: 0000-0002-7843-615X AD - Centro Oncologico Veterinario, Bologna, Italy. LA - eng GR - UNIMI linea B 2017/ PT - Comparative Study PT - Journal Article DEP - 20190204 PL - England TA - Vet Comp Oncol JT - Veterinary and comparative oncology JID - 101185242 SB - IM MH - Animals MH - Dog Diseases/drug therapy/*pathology MH - Dogs MH - Female MH - Flow Cytometry/veterinary MH - Lymph Nodes/drug effects/pathology MH - Lymphoma, Large B-Cell, Diffuse/drug therapy/pathology/*veterinary MH - Male MH - Neoplasm Recurrence, Local/drug therapy/pathology/*veterinary MH - Neoplasm, Residual MH - Prognosis MH - Proportional Hazards Models MH - Prospective Studies MH - Remission Induction MH - Time OTO - NOTNLM OT - canine OT - end-staging OT - flow cytometry OT - lymphoma OT - prognosis OT - relapse EDAT- 2018/12/24 06:00 MHDA- 2019/06/18 06:00 CRDT- 2018/12/23 06:00 PHST- 2018/08/28 00:00 [received] PHST- 2018/12/07 00:00 [revised] PHST- 2018/12/17 00:00 [accepted] PHST- 2018/12/24 06:00 [pubmed] PHST- 2019/06/18 06:00 [medline] PHST- 2018/12/23 06:00 [entrez] AID - 10.1111/vco.12453 [doi] PST - ppublish SO - Vet Comp Oncol. 2019 Jun;17(2):139-146. doi: 10.1111/vco.12453. Epub 2019 Feb 4.