PMID- 30581316
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1319-562X (Print)
IS  - 2213-7106 (Electronic)
IS  - 2213-7106 (Linking)
VI  - 25
IP  - 8
DP  - 2018 Dec
TI  - MHC binding peptides for designing of vaccines against Japanese encephalitis 
      virus: A computational approach.
PG  - 1546-1551
LID - 10.1016/j.sjbs.2016.01.041 [doi]
AB  - Japanese encephalitis (JE), a viral disease has seen a drastic and fatal 
      enlargement in the northern states of India in the current decade. The better and 
      exact cure for the disease is still in waiting. For the cause an in silico 
      strategy in the development of the peptide vaccine has been taken here for the 
      study. A computational approach to find out the Major Histocompatibility Complex 
      (MHC) binding peptide has been implemented. The prediction analysis identified 
      MHC class I (using propred I) and MHC class II (using propred) binding peptides 
      at an expectable percent predicted IC (50) threshold values. These predicted 
      Human leukocyte antigen [HLA] allele binding peptides were further analyzed for 
      potential conserved region using an Immune Epitope Database and Analysis Resource 
      (IEDB). This analysis shows that HLA-DRB1*0101, HLA-DRB3*0101, HLA-DRB1*0401, 
      HLA-DRB1*0102 and HLA-DRB1*07:01% of class II (in genotype 2) and HLA-A*0101, 
      HLA-A*02, HLA-A*0301, HLA-A*2402, HLA-B*0702 and HLA-B*4402% of HLA I (in 
      genotype 3) bound peptides are conserved. The predicted peptides MHC class I are 
      ILDSNGDIIGLY, FVMDEAHFTDPA, KTRKILPQIIK, RLMSPNRVPNYNLF, APTRVVAAEMAEAL, 
      YENVFHTLW and MHC class II molecule are TTGVYRIMARGILGT, NYNLFVMDEAHFTDP, 
      AAAIFMTATPPGTTD, GDTTTGVYRIMARGI and FGEVGAVSL found to be top ranking with 
      potential super antigenic property by binding to all HLA. Out of these the 
      predicted peptide FVMDEAHFTDPA for allele HLA-A*02:01 in MHC class I and 
      NYNLFVMDEAHFTDP for allele HLA-DRB3*01:01 in MHC class II was observed to be most 
      potent and can be further proposed as a significant vaccine in the process. The 
      reported results revealed that the immune-informatics techniques implemented in 
      the development of small size peptide is useful in the development of vaccines 
      against the Japanese encephalitis virus (JEV).
FAU - Sayeed, Usman
AU  - Sayeed U
AD  - Department of Biosciences, Faculty of Applied Sciences, Integral University, 
      Lucknow 226026, Uttar Pradesh, India.
FAU - Wadhwa, Gulshan
AU  - Wadhwa G
AD  - Department of Biotechnology, Ministry of Science and Technology, CGO Complex, 
      Lodhi Road, New Delhi 110003, India.
FAU - Sajid Jamal, Qazi Mohd
AU  - Sajid Jamal QM
AD  - Department of Health Information Management, College of Applied Medical Sciences, 
      Buraydah Colleges, Al Qassim-Buraydah King Abdul Aziz Road, East Qassim 
      University, Saudi Arabia.
FAU - Kamal, Mohammad A
AU  - Kamal MA
AD  - King Fahd Medical Research Center, King Abdulaziz University, Saudi Arabia.
AD  - Enzymoics, Peterlee Place, Hebersham, NSW 2770, Australia.
FAU - Akhtar, Salman
AU  - Akhtar S
AD  - Department of Bioengineering, Faculty of Engineering, Integral University, 
      Lucknow 226026, Uttar Pradesh, India.
FAU - Siddiqui, Mohd Haris
AU  - Siddiqui MH
AD  - Department of Bioengineering, Faculty of Engineering, Integral University, 
      Lucknow 226026, Uttar Pradesh, India.
FAU - Khan, M Salman
AU  - Khan MS
AD  - Department of Biosciences, Faculty of Applied Sciences, Integral University, 
      Lucknow 226026, Uttar Pradesh, India.
LA  - eng
PT  - Journal Article
DEP - 20160203
PL  - Saudi Arabia
TA  - Saudi J Biol Sci
JT  - Saudi journal of biological sciences
JID - 101543796
PMC - PMC6302886
OTO - NOTNLM
OT  - Epitope
OT  - Japanese encephalitis
OT  - MHC
OT  - Peptide
OT  - Vaccine
EDAT- 2018/12/26 06:00
MHDA- 2018/12/26 06:01
PMCR- 2016/02/03
CRDT- 2018/12/25 06:00
PHST- 2015/08/13 00:00 [received]
PHST- 2016/01/21 00:00 [revised]
PHST- 2016/01/27 00:00 [accepted]
PHST- 2018/12/25 06:00 [entrez]
PHST- 2018/12/26 06:00 [pubmed]
PHST- 2018/12/26 06:01 [medline]
PHST- 2016/02/03 00:00 [pmc-release]
AID - S1319-562X(16)00043-7 [pii]
AID - 10.1016/j.sjbs.2016.01.041 [doi]
PST - ppublish
SO  - Saudi J Biol Sci. 2018 Dec;25(8):1546-1551. doi: 10.1016/j.sjbs.2016.01.041. Epub 
      2016 Feb 3.