PMID- 30581532
OWN - NLM
STAT- MEDLINE
DCOM- 20190122
LR  - 20211204
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2018
DP  - 2018
TI  - Methane-Rich Saline Ameliorates Sepsis-Induced Acute Kidney Injury through 
      Anti-Inflammation, Antioxidative, and Antiapoptosis Effects by Regulating 
      Endoplasmic Reticulum Stress.
PG  - 4756846
LID - 10.1155/2018/4756846 [doi]
LID - 4756846
AB  - Sepsis-induced acute kidney injury (AKI) is a severe complication of sepsis and 
      an important cause of mortality in septic patients. Previous investigations 
      showed that methane had protective properties against different diseases in 
      animal models. This study is aimed at investigating whether methane-rich saline 
      (MRS) has a protective effect against sepsis-induced AKI. Sepsis was induced in 
      wild-type C57BL/6 mice by cecal ligation and puncture (CLP), and the mice were 
      divided into three groups: a sham control group (sham), a surgery group with 
      saline intraperitoneal injection (i.p.) treatment (CLP + NS), and a surgery group 
      with MRS i.p. treatment (CLP + MRS). 24 h after the establishment of the sepsis, 
      the blood and kidney tissues of mice in all groups were collected. According to 
      the serum levels of blood urea nitrogen (BUN) and creatinine (CRE) and a 
      histologic analysis, which included hematoxylin-eosin (H&E) staining and periodic 
      acid-Schiff (PAS) staining, MRS treatment protected renal function and tissues 
      from acute injury. Additionally, MRS treatment significantly ameliorated 
      apoptosis, based on the levels of apoptosis-related protein makers, including 
      cleaved caspase-3 and cleaved PARP, and the levels of Bcl-2/Bax expression and 
      TUNEL staining. In addition, the endoplasmic reticulum (ER) stress-related 
      glucose-regulated protein 78 (GRP78)/activating transcription factor 4 
      (ATF4)/C/EBP homologous protein (CHOP)/caspase-12 apoptosis signaling pathway was 
      significantly suppressed in the CLP + MRS group. The levels of inflammation and 
      oxidative stress were also reduced after MRS treatment. These results showed that 
      MRS has the potential to ameliorate sepsis-induced acute kidney injury through 
      its anti-inflammatory, antioxidative, and antiapoptosis properties.
FAU - Jia, Yifan
AU  - Jia Y
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an Shaanxi 710061, China.
FAU - Li, Zeyu
AU  - Li Z
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an Shaanxi 710061, China.
FAU - Feng, Yang
AU  - Feng Y
AD  - Department of Immunology, Shaanxi University of Chinese Medicine, Xianyang 
      Shaanxi 712046, China.
FAU - Cui, Ruixia
AU  - Cui R
AUID- ORCID: 0000-0002-2700-7531
AD  - Department of ICU, The First Affiliated Hospital of Xi'an Jiaotong University, 
      Xi'an Shaanxi 710061, China.
FAU - Dong, Yanyan
AU  - Dong Y
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an Shaanxi 710061, China.
FAU - Zhang, Xing
AU  - Zhang X
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an Shaanxi 710061, China.
FAU - Xiang, Xiaohong
AU  - Xiang X
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an Shaanxi 710061, China.
FAU - Qu, Kai
AU  - Qu K
AUID- ORCID: 0000-0002-1138-3727
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an Shaanxi 710061, China.
FAU - Liu, Chang
AU  - Liu C
AUID- ORCID: 0000-0002-7318-1004
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an Shaanxi 710061, China.
AD  - Department of SICU, The First Affiliated Hospital of Xi'an Jiaotong University, 
      Xi'an Shaanxi 710061, China.
FAU - Zhang, Jingyao
AU  - Zhang J
AUID- ORCID: 0000-0002-8227-9396
AD  - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an Shaanxi 710061, China.
AD  - Department of SICU, The First Affiliated Hospital of Xi'an Jiaotong University, 
      Xi'an Shaanxi 710061, China.
LA  - eng
PT  - Journal Article
DEP - 20181115
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Hspa5 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Saline Solution)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Acute Kidney Injury/*drug therapy/immunology/*metabolism
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress/drug effects
MH  - Female
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Inflammation/*drug therapy/immunology/metabolism
MH  - Methane/*chemistry/*therapeutic use
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Reactive Oxygen Species/metabolism
MH  - Saline Solution/chemistry/*therapeutic use
MH  - Sepsis/drug therapy/immunology/metabolism
PMC - PMC6276407
EDAT- 2018/12/26 06:00
MHDA- 2019/01/23 06:00
PMCR- 2018/11/15
CRDT- 2018/12/25 06:00
PHST- 2018/07/14 00:00 [received]
PHST- 2018/09/14 00:00 [accepted]
PHST- 2018/12/25 06:00 [entrez]
PHST- 2018/12/26 06:00 [pubmed]
PHST- 2019/01/23 06:00 [medline]
PHST- 2018/11/15 00:00 [pmc-release]
AID - 10.1155/2018/4756846 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2018 Nov 15;2018:4756846. doi: 10.1155/2018/4756846. 
      eCollection 2018.