PMID- 30583149 OWN - NLM STAT- MEDLINE DCOM- 20200518 LR - 20200518 IS - 1878-5905 (Electronic) IS - 0142-9612 (Linking) VI - 194 DP - 2019 Feb TI - Mussel-inspired injectable hydrogel and its counterpart for actuating proliferation and neuronal differentiation of retinal progenitor cells. PG - 57-72 LID - S0142-9612(18)30843-3 [pii] LID - 10.1016/j.biomaterials.2018.12.015 [doi] AB - Biomaterials-mediated retinal progenitor cell (RPC)-based transplantation therapy has shown substantial potential for retinal degeneration (RD), but it is limited by the poor RPC survival, proliferation and differentiation. Herein, the gelatin-hyaluronic acid (Gel-HA)-based hydrogels formed via moderate Michael-type addition reaction with or without the introduction of mussel-inspired polydopamine (PDA), i.e. Gel-HA-PDA and its counterpart Gel-HA hydrogels are developed, and their effects on the biological behaviour of RPCs, including adhesion, survival, proliferation, differentiation, delivery and migration are investigated. The hybrid hydrogels can adopt the intricate structure of the retina with suitable mechanical strength, degradation rate and biological activity to support cellular adhesion, survival and delivery. Meanwhile, Gel-HA hydrogel can remarkably promote RPC proliferation with much larger cell clusters, while Gel-HA-PDA hydrogel significantly enhances RPC adhesion and migration, and directs RPCs to preferentially differentiate toward retinal neurons such as photoreceptors (the most crucial cell-type for RD treatment), which is mainly induced by the activation of integrin alpha5beta1-phosphatidylinositol-3-kinase (PI3K) pathway. This study demonstrates that Gel-HA hydrogel possesses great potential for RPC proliferation, while mussel-inspired PDA-modified Gel-HA hydrogel with superior biocompatibility can significantly promote RPC neuronal differentiation, providing new insights for developing biomedical materials applied for RPC-based transplantation therapy. CI - Copyright (c) 2018 Elsevier Ltd. All rights reserved. FAU - Tang, Zhimin AU - Tang Z AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. FAU - Jiang, Fang AU - Jiang F AD - Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237, PR China. FAU - Zhang, Yuanhao AU - Zhang Y AD - Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237, PR China. FAU - Zhang, Yi AU - Zhang Y AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. FAU - YuanYang AU - YuanYang AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. FAU - Huang, Xiaolin AU - Huang X AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. FAU - Wang, Yuyao AU - Wang Y AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. FAU - Zhang, Dandan AU - Zhang D AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. FAU - Ni, Ni AU - Ni N AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. FAU - Liu, Feng AU - Liu F AD - Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237, PR China. FAU - Luo, Min AU - Luo M AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. FAU - Fan, Xianqun AU - Fan X AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. Electronic address: drfanxianqun@126.com. FAU - Zhang, Weian AU - Zhang W AD - Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, Shanghai, 200237, PR China. Electronic address: wazhang@ecust.edu.cn. FAU - Gu, Ping AU - Gu P AD - Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, PR China. Electronic address: guping2009@126.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20181215 PL - Netherlands TA - Biomaterials JT - Biomaterials JID - 8100316 RN - 0 (Biocompatible Materials) RN - 0 (Hydrogels) RN - 0 (Indoles) RN - 0 (Polymers) RN - 0 (polydopamine) RN - 9000-70-8 (Gelatin) RN - 9004-61-9 (Hyaluronic Acid) SB - IM MH - Animals MH - Biocompatible Materials/administration & dosage/*chemistry MH - Biomimetic Materials/administration & dosage/chemistry MH - Bivalvia/chemistry MH - Cell Adhesion MH - Cell Proliferation MH - Cell Survival MH - Cells, Cultured MH - Gelatin/administration & dosage/chemistry MH - Hyaluronic Acid/administration & dosage/chemistry MH - Hydrogels/administration & dosage/*chemistry MH - Indoles/administration & dosage/*chemistry MH - Injections MH - Mice, Inbred C57BL MH - Neurogenesis MH - Polymers/administration & dosage/*chemistry MH - Retina/*cytology MH - Stem Cells/*cytology OTO - NOTNLM OT - Differentiation OT - Hydrogel OT - Polydopamine OT - Proliferation OT - Retinal progenitor cells EDAT- 2018/12/26 06:00 MHDA- 2020/05/19 06:00 CRDT- 2018/12/25 06:00 PHST- 2018/07/28 00:00 [received] PHST- 2018/12/11 00:00 [revised] PHST- 2018/12/14 00:00 [accepted] PHST- 2018/12/26 06:00 [pubmed] PHST- 2020/05/19 06:00 [medline] PHST- 2018/12/25 06:00 [entrez] AID - S0142-9612(18)30843-3 [pii] AID - 10.1016/j.biomaterials.2018.12.015 [doi] PST - ppublish SO - Biomaterials. 2019 Feb;194:57-72. doi: 10.1016/j.biomaterials.2018.12.015. Epub 2018 Dec 15.