PMID- 30585263
OWN - NLM
STAT- MEDLINE
DCOM- 20190909
LR  - 20200309
IS  - 1449-2288 (Electronic)
IS  - 1449-2288 (Linking)
VI  - 14
IP  - 14
DP  - 2018
TI  - SAK-HV Promotes RAW264.7 cells Migration Mediated by MCP-1 via JNK and NF-kappaB 
      Pathways.
PG  - 1993-2002
LID - 10.7150/ijbs.27459 [doi]
AB  - Macrophage migration plays an essential role in immune system and is also 
      involved in many pathological situations. However, the regulatory mechanism of 
      macrophage migration remains to be elucidated due to its diverse responses to 
      various stimuli. SAK-HV, a multifunctional protein possessing thrombolytic and 
      lipid-lowering activity, can selectively induce the macrophage proliferation. 
      Here, we reported SAK-HV significantly triggered RAW264.7 cells migration through 
      its functional domain of SAK-mutant by activating both c-jun N-terminal kinases 
      (JNK) and nuclear factor-kappaB (NF-kappaB) pathways. Meanwhile, SAK-HV upregulated the 
      expression of some effector proteins, among which only the expression of Monocyte 
      chemoattractant protein-1 (MCP-1) was inhibited by the blockade of JNK and NF-kappaB 
      pathways. Further research showed that MCP-1 promoted migration ultimately by 
      interacting with Chemokine (C-C motif) Receptor 2 (CCR2) in an autocrine manner. 
      In summary, SAK-HV induced RAW264.7 cells migration through its SAK-mutant 
      domain, during which MCP-1 chemokine mediated by JNK and NF-kappaB pathways played a 
      key role. These results revealed a novel effect of SAK-HV on modulating 
      macrophage migration and also deepened the understanding of its pharmacodynamics.
FAU - Chen, Yao
AU  - Chen Y
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
FAU - Fu, Wen-Liang
AU  - Fu WL
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
FAU - Gan, Xiang-Dong
AU  - Gan XD
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
FAU - Xing, Wei-Wei
AU  - Xing WW
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
FAU - Xia, Wen-Rong
AU  - Xia WR
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
FAU - Zou, Min-Ji
AU  - Zou MJ
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
FAU - Liu, Qing
AU  - Liu Q
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
FAU - Wang, Yuan-Yuan
AU  - Wang YY
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
FAU - Zhang, Chao
AU  - Zhang C
AD  - Division of Oncology Research, Mayo Clinic, Rochester, MN 55905, USA.
FAU - Xu, Dong-Gang
AU  - Xu DG
AD  - Institute of Military Cognitive and Brain Sciences, Beijing, 100850, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181102
PL  - Australia
TA  - Int J Biol Sci
JT  - International journal of biological sciences
JID - 101235568
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, CCR2)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Movement/genetics/*physiology
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - JNK Mitogen-Activated Protein Kinases/genetics/metabolism
MH  - Macrophages/metabolism
MH  - Male
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/genetics/metabolism
MH  - NF-kappa B/genetics/metabolism
MH  - Phosphorylation/genetics/physiology
MH  - RAW 264.7 Cells
MH  - RNA, Small Interfering/genetics
MH  - Receptors, CCR2/genetics
MH  - Signal Transduction/genetics/physiology
MH  - Transfection
MH  - Wound Healing/genetics/physiology
PMC - PMC6299369
OTO - NOTNLM
OT  - JNK
OT  - MCP-1
OT  - NF-kappaB
OT  - SAK-HV
OT  - macrophage migration
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2018/12/27 06:00
MHDA- 2019/09/10 06:00
PMCR- 2018/01/01
CRDT- 2018/12/27 06:00
PHST- 2018/05/24 00:00 [received]
PHST- 2018/09/25 00:00 [accepted]
PHST- 2018/12/27 06:00 [entrez]
PHST- 2018/12/27 06:00 [pubmed]
PHST- 2019/09/10 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - ijbsv14p1993 [pii]
AID - 10.7150/ijbs.27459 [doi]
PST - epublish
SO  - Int J Biol Sci. 2018 Nov 2;14(14):1993-2002. doi: 10.7150/ijbs.27459. eCollection 
      2018.