PMID- 30585624
OWN - NLM
STAT- MEDLINE
DCOM- 20200218
LR  - 20200501
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 196
IP  - 2
DP  - 2019 May
TI  - Bronchoalveolar lavage characteristics correlate with HLA tag SNPs in patients 
      with Lofgren's syndrome and other sarcoidosis.
PG  - 249-258
LID - 10.1111/cei.13257 [doi]
AB  - Genetic susceptibility for sarcoidosis and Lofgren's syndrome (LS) has been 
      associated with prognosis. Human leukocyte antigen (HLA)-DRB1*03 is 
      over-represented in LS, and is associated with a good prognosis, whereas 
      HLA-DRB1*15-positive patients have a more chronic course of sarcoidosis. These 
      HLA-DRB1 types can be easily tagged by single nucleotide polymorphisms (SNPs). 
      Our aim was to evaluate the association between these tag SNPs and 
      bronchoalveolar lavage (BAL) characteristics. In 29 patients, both complete 
      HLA-DRB1* locus genotyping and SNP tagging was performed in parallel. HLA-DRB1 
      type was inferred from the presence of *03 tag rs2040410 allele A and referred to 
      as *03. HLA-DRB1*15 was inferred from the presence of tag SNP rs3135388 allele A 
      and referred to as *15. For BAL analysis, 122 patients with LS and 165 patients 
      with non-LS sarcoidosis were included. BAL lymphocyte subsets were analyzed by 
      flow cytometry. The presence of tag SNPs completely corresponded with 
      HLA-DRB1*03/*15 genotypes in all 29 patients in whom both HLA-DRB1* genotyping 
      and SNP tagging was performed. In all patients together, *03(+) /*15(-) patients 
      showed a higher CD4(+) /CD8(+) ratio than *03(-) /*15(+) (P = 0.004) and *03(-) 
      /*15(-) (P = 0.001). LS patients with *03(+) /*15(-) had a lower BAL lymphocyte 
      count compared to *03(-) /*15(+)  patients (P = 0.011). Non-LS sarcoidosis 
      patients with *03(+) /*15(-) patients showed a decreased CD103(+) CD4(+) /CD4(+) 
      ratio compared to *03(-) /*15(+)  patients (P = 0.045) and *03(-) /*15(-) 
      patients (P = 0.018). We found that HLA-DRB1*03 and HLA-DRB1*15 can be 
      approximated by genotyping of tag SNPs and corresponds with the degree of 
      lymphocytosis and cell phenotypes in BAL in both LS and non-LS sarcoidosis 
      patients.
CI  - (c) 2018 British Society for Immunology.
FAU - Karakaya, B
AU  - Karakaya B
AD  - Department of Pulmonology, ILD Center of Excellence, St Antonius Hospital, 
      Nieuwegein, the Netherlands.
FAU - Schimmelpennink, M C
AU  - Schimmelpennink MC
AUID- ORCID: 0000-0003-3222-1644
AD  - Department of Pulmonology, ILD Center of Excellence, St Antonius Hospital, 
      Nieuwegein, the Netherlands.
FAU - Kocourkova, L
AU  - Kocourkova L
AD  - Department of Pathological Physiology, Faculty of Medicine and Dentistry, Palacky 
      University Olomouc, Czech Republic.
FAU - van der Vis, J J
AU  - van der Vis JJ
AD  - Department of Pulmonology, ILD Center of Excellence, St Antonius Hospital, 
      Nieuwegein, the Netherlands.
AD  - Department of Clinical Chemistry, ILD Center of Excellence, St Antonius Hospital, 
      Nieuwegein, the Netherlands.
FAU - Meek, B
AU  - Meek B
AD  - Medical Immunology and Microbiology Department, St Antonius Hospital, Nieuwegein, 
      the Netherlands.
FAU - Grutters, J C
AU  - Grutters JC
AD  - Department of Pulmonology, ILD Center of Excellence, St Antonius Hospital, 
      Nieuwegein, the Netherlands.
AD  - Division of Heart and Lungs, University Medical Center, Utrecht, the Netherlands.
FAU - Petrek, M
AU  - Petrek M
AD  - Department of Pathological Physiology, Faculty of Medicine and Dentistry, Palacky 
      University Olomouc, Czech Republic.
AD  - University Hospital Olomouc, Olomouc, the Czech Republic.
FAU - van Moorsel, C H M
AU  - van Moorsel CHM
AD  - Department of Pulmonology, ILD Center of Excellence, St Antonius Hospital, 
      Nieuwegein, the Netherlands.
AD  - Division of Heart and Lungs, University Medical Center, Utrecht, the Netherlands.
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20190124
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*15 antigen)
SB  - IM
MH  - Adult
MH  - *Alleles
MH  - Bronchoalveolar Lavage
MH  - *CD4-Positive T-Lymphocytes/immunology/pathology
MH  - *CD8-Positive T-Lymphocytes/immunology/pathology
MH  - Genotyping Techniques
MH  - *HLA-DRB1 Chains/genetics/immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
MH  - *Sarcoidosis, Pulmonary/genetics/immunology/pathology
PMC - PMC6468184
OTO - NOTNLM
OT  - MHC
OT  - T cells
OT  - lung
COIS- The authors declare no potential conflicts of interest.
EDAT- 2018/12/27 06:00
MHDA- 2020/02/19 06:00
PMCR- 2020/05/01
CRDT- 2018/12/27 06:00
PHST- 2018/12/08 00:00 [accepted]
PHST- 2018/12/27 06:00 [pubmed]
PHST- 2020/02/19 06:00 [medline]
PHST- 2018/12/27 06:00 [entrez]
PHST- 2020/05/01 00:00 [pmc-release]
AID - CEI13257 [pii]
AID - 10.1111/cei.13257 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2019 May;196(2):249-258. doi: 10.1111/cei.13257. Epub 2019 Jan 
      24.