PMID- 30586464
OWN - NLM
STAT- MEDLINE
DCOM- 20190603
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 12
DP  - 2018
TI  - Bacterial diversity in the water column of meromictic Lake Cadagno and evidence 
      for seasonal dynamics.
PG  - e0209743
LID - 10.1371/journal.pone.0209743 [doi]
LID - e0209743
AB  - The meromictic Lake Cadagno is characterized by a compact chemocline with high 
      concentrations of anoxygenic phototrophic purple and green sulfur bacteria. 
      However, a complete picture of the bacterial diversity, and in particular of 
      effects of seasonality and compartmentalization is missing. To characterize 
      bacterial communities and elucidate relationships between them and their 
      surrounding environment high-throughput 16S rRNA gene pyrosequencing was 
      conducted. Proteobacteria, Chlorobi, Verrucomicrobia, and Actinobacteria were the 
      dominant groups in Lake Cadagno water column. Moreover, bacterial interaction 
      within the chemocline and between oxic and anoxic lake compartments were 
      investigated through fluorescence in situ hybridization (FISH) and flow cytometry 
      (FCM). The different populations of purple sulfur bacteria (PSB) and green sulfur 
      bacteria (GSB) in the chemocline indicate seasonal dynamics of phototrophic 
      sulfur bacteria composition. Interestingly, an exceptional bloom of a 
      cyanobacteria population in the oxic-anoxic transition zone affected the common 
      spatial distribution of phototrophic sulfur bacteria with consequence on 
      chemocline location and water column stability. Our study suggests that both 
      bacterial interactions between different lake compartments and within the 
      chemocline can be a dynamic process influencing the stratification structure of 
      Lake Cadagno water column.
FAU - Danza, Francesco
AU  - Danza F
AD  - Laboratory of Applied Microbiology (LMA), Department for Environmental 
      Constructions and Design (DACD), University of Applied Sciences and Arts of 
      Southern Switzerland (SUPSI), Bellinzona, Switzerland.
AD  - Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, 
      Geneva, Switzerland.
FAU - Ravasi, Damiana
AU  - Ravasi D
AD  - Laboratory of Applied Microbiology (LMA), Department for Environmental 
      Constructions and Design (DACD), University of Applied Sciences and Arts of 
      Southern Switzerland (SUPSI), Bellinzona, Switzerland.
FAU - Storelli, Nicola
AU  - Storelli N
AD  - Laboratory of Applied Microbiology (LMA), Department for Environmental 
      Constructions and Design (DACD), University of Applied Sciences and Arts of 
      Southern Switzerland (SUPSI), Bellinzona, Switzerland.
FAU - Roman, Samuele
AU  - Roman S
AD  - Laboratory of Applied Microbiology (LMA), Department for Environmental 
      Constructions and Design (DACD), University of Applied Sciences and Arts of 
      Southern Switzerland (SUPSI), Bellinzona, Switzerland.
AD  - Alpine Biology Center Foundation, Bellinzona, Switzerland.
FAU - Ludin, Samuel
AU  - Ludin S
AUID- ORCID: 0000-0002-2803-2536
AD  - Laboratory of Applied Microbiology (LMA), Department for Environmental 
      Constructions and Design (DACD), University of Applied Sciences and Arts of 
      Southern Switzerland (SUPSI), Bellinzona, Switzerland.
AD  - Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, 
      Geneva, Switzerland.
AD  - Federal Office for Civil Protection, Spiez Laboratory, Biology Division, Spiez, 
      Switzerland.
FAU - Bueche, Matthieu
AU  - Bueche M
AD  - Institute of Chemistry, University of Neuchatel, Neuchatel, Switzerland.
AD  - Bueche mulab, Le Paquier, Switzerland.
FAU - Tonolla, Mauro
AU  - Tonolla M
AD  - Laboratory of Applied Microbiology (LMA), Department for Environmental 
      Constructions and Design (DACD), University of Applied Sciences and Arts of 
      Southern Switzerland (SUPSI), Bellinzona, Switzerland.
AD  - Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, 
      Geneva, Switzerland.
AD  - Alpine Biology Center Foundation, Bellinzona, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181226
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Chlorobi/classification/genetics
MH  - Chromatiaceae/classification/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Lakes/*microbiology
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Seasons
MH  - Water Microbiology
PMC - PMC6306205
COIS- We have the following interests. Matthieu Bueche is affiliated to Bueche mulab. 
      There are no patents, products in development or marketed products to declare. 
      This does not alter our adherence to all the PLOS ONE policies on sharing data 
      and materials, as detailed online in the guide for authors.
EDAT- 2018/12/27 06:00
MHDA- 2019/06/04 06:00
PMCR- 2018/12/26
CRDT- 2018/12/27 06:00
PHST- 2018/02/27 00:00 [received]
PHST- 2018/12/11 00:00 [accepted]
PHST- 2018/12/27 06:00 [entrez]
PHST- 2018/12/27 06:00 [pubmed]
PHST- 2019/06/04 06:00 [medline]
PHST- 2018/12/26 00:00 [pmc-release]
AID - PONE-D-18-06240 [pii]
AID - 10.1371/journal.pone.0209743 [doi]
PST - epublish
SO  - PLoS One. 2018 Dec 26;13(12):e0209743. doi: 10.1371/journal.pone.0209743. 
      eCollection 2018.