PMID- 30586652
OWN - NLM
STAT- MEDLINE
DCOM- 20190509
LR  - 20190509
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 123
DP  - 2019 Apr
TI  - Obestatin may affect the GnRH/KNDy gene network in sheep hypothalamus.
PG  - 51-58
LID - S0034-5288(18)30243-1 [pii]
LID - 10.1016/j.rvsc.2018.12.010 [doi]
AB  - The effects of obestatin on gonadotrophic axis activity in ruminants have not yet 
      been determined. The aim of this study was to investigate the effect of 
      intracerebroventricular infusions of obestatin on the gonadotrophin-releasing 
      hormone (GnRH) mRNA and protein expressions as well as on KNDy mRNA and 
      kisspeptin (Kiss) peptide expressions in peripubertal female sheep. Animals were 
      randomly divided into two groups: the control group received 
      intracerebroventricular infusions of the vehicle, and the obestatin group was 
      infused with obestatin (25 mug/120 muL h(-1)). The series of four 1-h infusions per 
      day during three consecutive days were performed. After the end of the experiment 
      parts of sheep brains were fixed in situ for immunohistochemical analysis, while 
      the remaining brains were frozen for Real Time qPCR analysis. Substantial changes 
      in the activity of the GnRH and KNDy gene network were observed in 
      obestatin-infused sheep. In those animals an increase of GnRH mRNA expression in 
      the preoptic area, a decrease of GnRH mRNA expression in the median eminence and 
      an increase of GnRH immunoreactivity in the median eminence were found. Moreover, 
      changes in the KNDy mRNA expression in mediobasal hypothalamus as well as 
      decrease Kiss expression in arcuate nucleus and median eminence were observed. It 
      was revealed that obestatin affects the GnRH and KNDy gene network as well as 
      Kiss at the level of mRNA and protein expression. Thereby, it can be concluded 
      that obestatin participates in the mechanism modulating gonadotrophic axis 
      activity at the central level in peripubertal female sheep.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Wojcik-Gladysz, Anna
AU  - Wojcik-Gladysz A
AD  - Department of Animal Physiology, The Kielanowski Institute of Animal Physiology 
      and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland.
FAU - Szlis, Michal
AU  - Szlis M
AD  - Department of Animal Physiology, The Kielanowski Institute of Animal Physiology 
      and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland. Electronic 
      address: m.szlis@ifzz.pl.
FAU - Przybyl, Bartosz Jaroslaw
AU  - Przybyl BJ
AD  - Department of Animal Physiology, The Kielanowski Institute of Animal Physiology 
      and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland.
FAU - Polkowska, Jolanta
AU  - Polkowska J
AD  - Department of Animal Physiology, The Kielanowski Institute of Animal Physiology 
      and Nutrition, Polish Academy of Sciences, 05-110 Jablonna, Poland.
LA  - eng
PT  - Journal Article
DEP - 20181219
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Ghrelin)
RN  - 0 (RNA, Messenger)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Ghrelin/administration & dosage/metabolism/*pharmacology
MH  - Gonadotropin-Releasing Hormone/drug effects/genetics/*metabolism
MH  - Hypothalamus/diagnostic imaging/*drug effects/metabolism
MH  - Immunohistochemistry
MH  - Infusions, Intraventricular
MH  - RNA, Messenger/metabolism
MH  - Random Allocation
MH  - Real-Time Polymerase Chain Reaction
MH  - Sexual Maturation
MH  - Sheep/*physiology
OTO - NOTNLM
OT  - GnRH
OT  - Hypothalamus
OT  - KNDy genes
OT  - Kisspeptin
OT  - Obestatin
OT  - Sheep
EDAT- 2018/12/27 06:00
MHDA- 2019/05/10 06:00
CRDT- 2018/12/27 06:00
PHST- 2018/03/09 00:00 [received]
PHST- 2018/12/15 00:00 [revised]
PHST- 2018/12/17 00:00 [accepted]
PHST- 2018/12/27 06:00 [pubmed]
PHST- 2019/05/10 06:00 [medline]
PHST- 2018/12/27 06:00 [entrez]
AID - S0034-5288(18)30243-1 [pii]
AID - 10.1016/j.rvsc.2018.12.010 [doi]
PST - ppublish
SO  - Res Vet Sci. 2019 Apr;123:51-58. doi: 10.1016/j.rvsc.2018.12.010. Epub 2018 Dec 
      19.