PMID- 30586858
OWN - NLM
STAT- MEDLINE
DCOM- 20190403
LR  - 20200225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 1
DP  - 2018 Dec 24
TI  - Catechol 1,2-Dioxygenase is an Analogue of Homogentisate 1,2-Dioxygenase in 
      Pseudomonas chlororaphis Strain UFB2.
LID - 10.3390/ijms20010061 [doi]
LID - 61
AB  - Catechol dioxygenases in microorganisms cleave catechol into cis-cis-muconic acid 
      or 2-hydroxymuconic semialdehyde via the ortho- or meta-pathways, respectively. 
      The aim of this study was to purify, characterize, and predict the template-based 
      three-dimensional structure of catechol 1,2-dioxygenase (C12O) from indigenous 
      Pseudomonas chlororaphis strain UFB2 (PcUFB2). Preliminary studies showed that 
      PcUFB2 could degrade 40 ppm of 2,4-dichlorophenol (2,4-DCP). The crude cell 
      extract showed 10.34 U/mL of C12O activity with a specific activity of 2.23 U/mg 
      of protein. A 35 kDa protein was purified to 1.5-fold with total yield of 13.02% 
      by applying anion exchange and gel filtration chromatography. The enzyme was 
      optimally active at pH 7.5 and a temperature of 30  degrees C. The Lineweaver(-)Burk plot 
      showed the v(max) and K(m) values of 16.67 microM/min and 35.76 microM, respectively. 
      ES-MS spectra of tryptic digested SDS-PAGE band and bioinformatics studies 
      revealed that C12O shared 81% homology with homogentisate 1,2-dioxygenase 
      reported in other Pseudomonas chlororaphis strains. The characterization and 
      optimization of C12O activity can assist in understanding the 2,4-DCP metabolic 
      pathway in PcUFB2 and its possible application in bioremediation strategies.
FAU - Setlhare, Boitumelo
AU  - Setlhare B
AUID- ORCID: 0000-0002-9502-5344
AD  - Discipline of Microbiology, School of Life Sciences, College of Agriculture, 
      Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private 
      Bag X54001, Durban 4000, South Africa. boitumellow5@gmail.com.
FAU - Kumar, Ajit
AU  - Kumar A
AD  - Discipline of Microbiology, School of Life Sciences, College of Agriculture, 
      Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private 
      Bag X54001, Durban 4000, South Africa. ajitkanwal@yahoo.com.
FAU - Mokoena, Mduduzi P
AU  - Mokoena MP
AD  - Discipline of Microbiology, School of Life Sciences, College of Agriculture, 
      Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private 
      Bag X54001, Durban 4000, South Africa. Mokoenap@ukzn.ac.za.
FAU - Olaniran, Ademola O
AU  - Olaniran AO
AD  - Discipline of Microbiology, School of Life Sciences, College of Agriculture, 
      Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private 
      Bag X54001, Durban 4000, South Africa. olanirana@ukzn.ac.za.
LA  - eng
GR  - 94036 and 92803/National Research Foundation/
PT  - Journal Article
DEP - 20181224
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Bacterial Proteins)
RN  - 0 (Catechols)
RN  - 0 (Chlorophenols)
RN  - 0 (Metals)
RN  - EC 1.13.11.1 (Catechol 1,2-Dioxygenase)
RN  - EC 1.13.11.5 (Homogentisate 1,2-Dioxygenase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/chemistry/classification/*metabolism
MH  - Catechol 1,2-Dioxygenase/chemistry/classification/*metabolism
MH  - Catechols/metabolism
MH  - Chlorophenols/chemistry/metabolism
MH  - Chromatography, Gel
MH  - Chromatography, Ion Exchange
MH  - Homogentisate 1,2-Dioxygenase/chemistry/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Metals/chemistry/metabolism
MH  - Phylogeny
MH  - Protein Stability
MH  - Protein Structure, Quaternary
MH  - Pseudomonas chlororaphis/*enzymology
MH  - Sequence Alignment
MH  - Substrate Specificity
MH  - Temperature
PMC - PMC6337169
OTO - NOTNLM
OT  - Pseudomonas chlororaphis
OT  - Pseudomonas chlororaphis strain UFB2
OT  - catechol 1,2-dioxygenase
OT  - homogentisate 1,2-dioxygenase
COIS- The authors declare no conflict of interest.
EDAT- 2018/12/28 06:00
MHDA- 2019/04/04 06:00
PMCR- 2019/01/01
CRDT- 2018/12/28 06:00
PHST- 2018/10/11 00:00 [received]
PHST- 2018/11/27 00:00 [revised]
PHST- 2018/11/29 00:00 [accepted]
PHST- 2018/12/28 06:00 [entrez]
PHST- 2018/12/28 06:00 [pubmed]
PHST- 2019/04/04 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - ijms20010061 [pii]
AID - ijms-20-00061 [pii]
AID - 10.3390/ijms20010061 [doi]
PST - epublish
SO  - Int J Mol Sci. 2018 Dec 24;20(1):61. doi: 10.3390/ijms20010061.