PMID- 30587512
OWN - NLM
STAT- MEDLINE
DCOM- 20191210
LR  - 20211204
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 218
IP  - 2
DP  - 2019 Feb 4
TI  - Autophagic degradation of lamins facilitates the nuclear egress of herpes simplex 
      virus type 1.
PG  - 508-523
LID - 10.1083/jcb.201801151 [doi]
AB  - Dendritic cells (DCs) are crucial for the induction of potent antiviral immune 
      responses. In contrast to immature DCs (iDCs), mature DCs (mDCs) are not 
      permissive for infection with herpes simplex virus type 1 (HSV-1). Here, we 
      demonstrate that HSV-1 infection of iDCs and mDCs induces autophagy, which 
      promotes the degradation of lamin A/C, B1, and B2 in iDCs only. This in turn 
      facilitates the nuclear egress of progeny viral capsids and thus the formation of 
      new infectious particles. In contrast, lamin protein levels remain stable in 
      HSV-1-infected mDCs due to an inefficient autophagic flux. Elevated protein 
      levels of KIF1B and KIF2A in mDCs inhibited lamin degradation, likely by 
      hampering autophagosome-lysosome fusion. Therefore, in mDCs, fewer progeny 
      capsids were released from the nuclei into the cytosol, and fewer infectious 
      virions were assembled. We hypothesize that inhibition of autophagic lamin 
      degradation in mDCs represents a very powerful cellular counterstrike to inhibit 
      the production of progeny virus and thus viral spread.
CI  - (c) 2019 Turan et al.
FAU - Turan, Aykut
AU  - Turan A
AUID- ORCID: 0000-0003-4259-8955
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Grosche, Linda
AU  - Grosche L
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Krawczyk, Adalbert
AU  - Krawczyk A
AUID- ORCID: 0000-0001-9502-9903
AD  - Institute for Virology, University Hospital Essen, University of Duisburg-Essen, 
      Essen, Germany.
FAU - Muhl-Zurbes, Petra
AU  - Muhl-Zurbes P
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Drassner, Christina
AU  - Drassner C
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Duthorn, Alexandra
AU  - Duthorn A
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Kummer, Mirko
AU  - Kummer M
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, 
      Germany.
FAU - Hasenberg, Mike
AU  - Hasenberg M
AUID- ORCID: 0000-0001-7466-1846
AD  - Imaging Center Essen, Electron Microscopy Unit, University Hospital of Essen, 
      Essen, Germany.
FAU - Voortmann, Sylvia
AU  - Voortmann S
AD  - Imaging Center Essen, Electron Microscopy Unit, University Hospital of Essen, 
      Essen, Germany.
FAU - Jastrow, Holger
AU  - Jastrow H
AD  - Imaging Center Essen, Electron Microscopy Unit, University Hospital of Essen, 
      Essen, Germany.
AD  - Institute of Anatomy, University of Duisburg-Essen, Essen, Germany.
FAU - Dorrie, Jan
AU  - Dorrie J
AUID- ORCID: 0000-0002-3478-0741
AD  - Department of Dermatology, Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Schaft, Niels
AU  - Schaft N
AUID- ORCID: 0000-0001-8236-9298
AD  - Department of Dermatology, Universitatsklinikum Erlangen, Erlangen, Germany.
FAU - Kraner, Max
AU  - Kraner M
AUID- ORCID: 0000-0003-1733-1909
AD  - Division of Biochemistry, Department of Biology, Friedrich-Alexander Universitat 
      Erlangen-Nurnberg, Erlangen, Germany.
FAU - Dohner, Katinka
AU  - Dohner K
AUID- ORCID: 0000-0002-8222-3085
AD  - Institute of Virology, Hannover Medical School, Hannover, Germany.
FAU - Sodeik, Beate
AU  - Sodeik B
AUID- ORCID: 0000-0003-4650-3036
AD  - Institute of Virology, Hannover Medical School, Hannover, Germany.
FAU - Steinkasserer, Alexander
AU  - Steinkasserer A
AUID- ORCID: 0000-0001-7187-6729
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, Germany 
      alexander.steinkasserer@uk-erlangen.de.
FAU - Heilingloh, Christiane Silke
AU  - Heilingloh CS
AD  - Department of Immune Modulation, Universitatsklinikum Erlangen, Erlangen, Germany 
      christiane.heilingloh@gmx.de.
LA  - eng
SI  - RefSeq/NC_001806
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181226
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (KIF1B protein, human)
RN  - 0 (KIF2A protein, human)
RN  - 0 (Lamins)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
CIN - J Cell Biol. 2019 Feb 4;218(2):387-388. PMID: 30647098
MH  - Capsid/*metabolism
MH  - *Cell Nucleus/metabolism/virology
MH  - *Cytosol/metabolism/virology
MH  - *Dendritic Cells/metabolism/virology
MH  - Herpesvirus 1, Human/genetics/*metabolism
MH  - Humans
MH  - Kinesins/genetics/metabolism
MH  - Lamins/genetics/metabolism
MH  - Proteolysis
MH  - Virus Release/*physiology
PMC - PMC6363456
EDAT- 2018/12/28 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/08/04
CRDT- 2018/12/28 06:00
PHST- 2018/01/22 00:00 [received]
PHST- 2018/10/02 00:00 [revised]
PHST- 2018/11/08 00:00 [accepted]
PHST- 2018/12/28 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2018/12/28 06:00 [entrez]
PHST- 2019/08/04 00:00 [pmc-release]
AID - jcb.201801151 [pii]
AID - 201801151 [pii]
AID - 10.1083/jcb.201801151 [doi]
PST - ppublish
SO  - J Cell Biol. 2019 Feb 4;218(2):508-523. doi: 10.1083/jcb.201801151. Epub 2018 Dec 
      26.