PMID- 30587686
OWN - NLM
STAT- MEDLINE
DCOM- 20190514
LR  - 20190514
IS  - 1880-4926 (Electronic)
IS  - 1343-4934 (Linking)
VI  - 60
IP  - 4
DP  - 2018
TI  - Hypoxia-induced upregulation of angiogenic factors in immortalized human 
      periodontal ligament fibroblasts.
PG  - 519-525
LID - 10.2334/josnusd.17-0441 [doi]
AB  - Hypoxia induces complex cellular responses that are mediated by a key 
      transcription factor, hypoxia-inducible factor-1 (HIF-1). HIF-1 promotes 
      production of cytokines and angiogenic factors and contributes to recovery of 
      injured tissues. In the present study, expressions of angiogenin (ANG) and 
      vascular endothelial growth factor (VEGF), which are potent angiogenic factors in 
      mammalian tissues, were examined in immortalized fibroblasts exposed to hypoxia. 
      After 24 h of exposure to hypoxia, ANG and VEGF mRNAs expressions were 
      significantly elevated in periodontal ligament (PDL) fibroblasts but not in 
      embryonic fibroblasts. Hypoxia also increased productions of ANG and VEGF 
      proteins in PDL fibroblasts. HIF-1alpha mRNA expression was not affected by hypoxia 
      in either fibroblast, although HIF-1alpha protein expression was enhanced after 
      exposure to hypoxia. Treatment of PDL fibroblasts with dimethyloxaloylglycine, a 
      prolyl hydroxylase inhibitor that stabilizes the HIF-1alpha protein, significantly 
      increased expressions of ANG and VEGF mRNAs under normoxia. This suggests that 
      stabilization of HIF-1alpha is crucial for upregulation of ANG and VEGF in PDL 
      fibroblasts. These results indicate that, under hypoxic conditions, HIF-1alpha 
      upregulates synthesis of ANG and VEGF in PDL fibroblasts and promotes 
      angiogenesis.
FAU - Kifune, Takashi
AU  - Kifune T
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Ito, Hisanori
AU  - Ito H
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Ishiyama, Misa
AU  - Ishiyama M
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Iwasa, Satoko
AU  - Iwasa S
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Takei, Hiroki
AU  - Takei H
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Hasegawa, Tomokazu
AU  - Hasegawa T
AD  - Department of Pediatric Dentistry, Tokushima University Hospital.
FAU - Asano, Masatake
AU  - Asano M
AD  - Department of Pathology, Nihon University School of Dentistry.
FAU - Shirakawa, Tetsuo
AU  - Shirakawa T
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
AD  - Division of Oral and Craniomaxillofacial Research, Dental Research Center, Nihon 
      University School of Dentistry.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Oral Sci
JT  - Journal of oral science
JID - 9808942
RN  - 0 (Cytokines)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 3.1.27.- (angiogenin)
RN  - EC 3.1.27.5 (Ribonuclease, Pancreatic)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Fibroblasts/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - *Hypoxia
MH  - Hypoxia-Inducible Factor 1/*metabolism
MH  - Periodontal Ligament/*cytology
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Ribonuclease, Pancreatic/*metabolism
MH  - Up-Regulation
MH  - Vascular Endothelial Growth Factor A/*metabolism
OTO - NOTNLM
OT  - HIF-1
OT  - angiogenin
OT  - hypoxia
OT  - periodontal ligament fibroblasts
OT  - vascular endothelial growth factor
EDAT- 2018/12/28 06:00
MHDA- 2019/05/15 06:00
CRDT- 2018/12/28 06:00
PHST- 2018/12/28 06:00 [entrez]
PHST- 2018/12/28 06:00 [pubmed]
PHST- 2019/05/15 06:00 [medline]
AID - 10.2334/josnusd.17-0441 [doi]
PST - ppublish
SO  - J Oral Sci. 2018;60(4):519-525. doi: 10.2334/josnusd.17-0441.