PMID- 30587688
OWN - NLM
STAT- MEDLINE
DCOM- 20190514
LR  - 20190514
IS  - 1880-4926 (Electronic)
IS  - 1343-4934 (Linking)
VI  - 60
IP  - 4
DP  - 2018
TI  - Effect of hypoxia on the expression of CCAAT/enhancer-binding protein beta and 
      receptor activator of nuclear factor kappa-B ligand in periodontal ligament 
      cells.
PG  - 544-551
LID - 10.2334/josnusd.17-0436 [doi]
AB  - Hypoxia after traumatic injuries to a tooth is one of the causes of subsequent 
      root resorption. Inflammatory cytokines produced under hypoxic conditions are 
      associated with root resorption, but the mechanism has not been fully understood. 
      In this study, the role of hypoxia-inducible factor-1 (HIF-1) signaling in the 
      regulation of CCAAT 
      (cytosine-cytosine-adenosine-adenosine-thymidine)/enhancer-binding protein-beta 
      (C/EBPbeta) and the receptor activator of nuclear factor kappa-B ligand (RANKL) 
      expressions in immortalized human periodontal ligament (PDL) cells was 
      investigated. PDL cells cultured under a hypoxic condition showed an increase in 
      the expression of C/EBPbeta and RANKL messenger RNAs (mRNAs), whereas the expression 
      of osteoprotegerin and HIF-1alpha mRNAs was unaffected. Hypoxia had no effects on the 
      secretion of interleukin (IL)-1beta, IL-6, IL-8, IL-17A, tumor necrosis 
      factor-alpha, macrophage migration inhibitory factor, monocyte chemoattractant 
      protein-1, and macrophage colony-stimulating factor in the culture media. 
      Treatment of the cells with dimethyloxaloylglycine, a competitive HIF prolyl 
      hydroxylase inhibitor, significantly increased the expression of C/EBPbeta and RANKL 
      mRNAs. This suggested that the hypoxia-induced elevation of C/EBPbeta and RANKL 
      mRNAs was dependent on the HIF-1 activity. PDL cells transfected with a specific 
      small interfering RNA designed to target the C/EBPbeta gene showed a significant 
      suppression of the RANKL mRNA. These findings indicated that C/EBPbeta may play an 
      important role in tooth root resorption via RANKL activation in hypoxia-exposed 
      PDL cells.
FAU - Ito, Hisanori
AU  - Ito H
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Kifune, Takashi
AU  - Kifune T
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Ishiyama, Misa
AU  - Ishiyama M
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Iwasa, Satoko
AU  - Iwasa S
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Takei, Hiroki
AU  - Takei H
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
FAU - Hasegawa, Tomokazu
AU  - Hasegawa T
AD  - Department of Pediatric Dentistry, Tokushima University Hospital.
FAU - Asano, Masatake
AU  - Asano M
AD  - Department of Pathology, Nihon University School of Dentistry.
FAU - Shirakawa, Tetsuo
AU  - Shirakawa T
AD  - Department of Pediatric Dentistry, Nihon University School of Dentistry.
AD  - Division of Oral and Craniomaxillofacial Research, Dental Research Center, Nihon 
      University School of Dentistry.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - J Oral Sci
JT  - Journal of oral science
JID - 9808942
RN  - 0 (Amino Acids, Dicarboxylic)
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - 0 (Cytokines)
RN  - 0 (RANK Ligand)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - VVW38EB8YS (oxalylglycine)
SB  - IM
MH  - Amino Acids, Dicarboxylic/pharmacology
MH  - Blotting, Western
MH  - CCAAT-Enhancer-Binding Protein-beta/*metabolism
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - *Hypoxia
MH  - Periodontal Ligament/*cytology
MH  - RANK Ligand/*metabolism
MH  - RNA, Messenger/metabolism
MH  - RNA, Small Interfering/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Transfection
OTO - NOTNLM
OT  - C/EBPbeta
OT  - HIF-1
OT  - RANKL
OT  - hypoxia
OT  - periodontal ligament cells
EDAT- 2018/12/28 06:00
MHDA- 2019/05/15 06:00
CRDT- 2018/12/28 06:00
PHST- 2018/12/28 06:00 [entrez]
PHST- 2018/12/28 06:00 [pubmed]
PHST- 2019/05/15 06:00 [medline]
AID - 10.2334/josnusd.17-0436 [doi]
PST - ppublish
SO  - J Oral Sci. 2018;60(4):544-551. doi: 10.2334/josnusd.17-0436.