PMID- 30590524
OWN - NLM
STAT- MEDLINE
DCOM- 20190321
LR  - 20190321
IS  - 1945-239X (Electronic)
IS  - 0021-9665 (Linking)
VI  - 57
IP  - 3
DP  - 2019 Mar 1
TI  - Fingerprint Analysis and Multi-Component Determination of Ribonucleic Acid for 
      Injection II Recipe by HPLC-DAD and LC-ESI-MS Methods.
PG  - 238-242
LID - 10.1093/chromsci/bmy105 [doi]
AB  - Using high-performance liquid chromatography (HPLC), a quantitative method was 
      developed to control the quality of ribonucleic acid for injection II (RA-II), 
      which is used as an anti-cancer drug clinically in China. Using nuclease enzyme 
      and under optimal hydrolysis conditions, the unstable RNA was hydrolyzed into 
      stable nucleosides and nucleotides, which were easily detected by HPLC with diode 
      array detection. Furthermore, by analyzing HPLC chromatograms of 10 batches of 
      samples, six common peaks, namely, cytosine nucleotide, uracil nucleotide, 
      adenine nucleotide, guanine nucleotide, guanosine and adenine, were identified by 
      a HLPC coupled with electrospray ionization mass spectrometry. The similarity, 
      the relative retention time and the relative peak area of each common peak, 
      relative to the reference peak, were calculated to obtain the HPLC fingerprints, 
      and their values were within the scope of the provisions. More importantly, all 
      six components were simultaneously determined. Overall, the developed method in 
      this investigation proved to be a useful tool for monitoring the quality of RA-II 
      in terms of their batch variations and the raw material sources.
FAU - Zheng, Wei
AU  - Zheng W
AD  - Departments of Ophthalmology, The Affiliated Hospital, Changchun University of 
      Chinese Medicine, No 1478, Gongnong Road, Changchun, Jilin, China.
FAU - Xu, Duoduo
AU  - Xu D
AD  - Center of Research & Development, Changchun University of Chinese Medicine, No 
      1035, Boshuo Road Changchun, Jilin, China.
AD  - Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin, 
      China.
FAU - Wang, Mingxing
AU  - Wang M
AD  - Preparation Center, The Affiliated Hospital, Changchun University of Chinese 
      Medicine, Changchun, Jilin, China.
FAU - Zhang, Yanqiu
AU  - Zhang Y
AD  - Preparation Center, The Affiliated Hospital, Changchun University of Chinese 
      Medicine, Changchun, Jilin, China.
FAU - Gao, Qipin
AU  - Gao Q
AD  - Center of Research & Development, Changchun University of Chinese Medicine, No 
      1035, Boshuo Road Changchun, Jilin, China.
AD  - Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin, 
      China.
FAU - Gao, Yang
AU  - Gao Y
AD  - Center of Research & Development, Changchun University of Chinese Medicine, No 
      1035, Boshuo Road Changchun, Jilin, China.
AD  - Macromolecule of Chinese Medicine Key Lab of Jilin Province, Changchun, Jilin, 
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Chromatogr Sci
JT  - Journal of chromatographic science
JID - 0173225
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - China
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Limit of Detection
MH  - Linear Models
MH  - RNA/analysis/*chemistry
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2018/12/28 06:00
MHDA- 2019/03/22 06:00
CRDT- 2018/12/28 06:00
PHST- 2017/10/10 00:00 [received]
PHST- 2018/12/28 06:00 [pubmed]
PHST- 2019/03/22 06:00 [medline]
PHST- 2018/12/28 06:00 [entrez]
AID - 5257451 [pii]
AID - 10.1093/chromsci/bmy105 [doi]
PST - ppublish
SO  - J Chromatogr Sci. 2019 Mar 1;57(3):238-242. doi: 10.1093/chromsci/bmy105.