PMID- 30592078 OWN - NLM STAT- MEDLINE DCOM- 20191220 LR - 20210430 IS - 1096-8652 (Electronic) IS - 0361-8609 (Print) IS - 0361-8609 (Linking) VI - 94 IP - 4 DP - 2019 Apr TI - Rapid assessment of hyperdiploidy in plasma cell disorders using a novel multi-parametric flow cytometry method. PG - 424-430 LID - 10.1002/ajh.25391 [doi] AB - Trisomies of odd numbered chromosomes are seen in nearly half of patients with multiple myeloma (MM) and typically correlate with a hyperdiploid state and better overall survival (OS). We compared DNA ploidy of monoclonal plasma cells (as a surrogate for the presence of trisomies) assessed simultaneously by PCPRO (plasma cell proliferative index), a novel method that estimates DNA index by multi-parametric flow cytometry to fluorescence in situ hybridization (FISH) in 1703 patients with plasma cell disorders. The distribution of ploidy was hyperdiploid: 759 (45%), diploid 765 (45%), hypodiploid: 71 (4%), tetraploid/near-tetraploid: 108 (6%). FISH identified trisomies in 82% (621/756) of patients with hyperdiploidy by PCPRO and no trisomy by FISH was observed in 88% (730/834) of patients without hyperdiploidy. 95% (795/834) of patients without hyperdiploidy on PCPRO had one or less trisomy by FISH. Sensitivity and specificity of PCPRO for detecting hyperdiploidy was 86% (621/725) and 84% (730/865), respectively. Sensitivity increased to 94% (579/618) for patients with more than one trisomy. Newly diagnosed MM patients with hyperdiploidy on PCPRO (147/275) had better OS compared to nonhyperdiploid patients (median not reached vs 59 months, P = 0.008) and better progression free survival (median: 33 vs 23 months, P = 0.03). Within the hyperdiploidy group, patients with high-hyperdiploidy (DNA index: 1.19-1.50) versus those with low-hyperdiploidy (DNA index: 1.05-1.18) had superior OS (3 year OS of 88% vs 68% P = 0.03). Ploidy assessment by flow cytometry can provide rapid, valuable prognostic information and also reduces the number of copy number FISH probes required and hence the cost of FISH. CI - (c) 2018 Wiley Periodicals, Inc. FAU - Sidana, Surbhi AU - Sidana S AUID- ORCID: 0000-0003-3288-7614 AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Jevremovic, Dragan AU - Jevremovic D AD - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota. FAU - Ketterling, Rhett P AU - Ketterling RP AD - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota. FAU - Tandon, Nidhi AU - Tandon N AUID- ORCID: 0000-0003-4008-9081 AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Dispenzieri, Angela AU - Dispenzieri A AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Gertz, Morie A AU - Gertz MA AUID- ORCID: 0000-0002-3853-5196 AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Greipp, Patricia T AU - Greipp PT AD - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota. FAU - Baughn, Linda B AU - Baughn LB AD - Division of Laboratory Genetics, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota. FAU - Buadi, Francis K AU - Buadi FK AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Lacy, Martha Q AU - Lacy MQ AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Morice, William AU - Morice W AD - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota. FAU - Hanson, Curtis AU - Hanson C AD - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota. FAU - Timm, Michael AU - Timm M AD - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota. FAU - Dingli, David AU - Dingli D AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Hayman, Suzanne R AU - Hayman SR AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Gonsalves, Wilson I AU - Gonsalves WI AUID- ORCID: 0000-0001-6890-969X AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Kapoor, Prashant AU - Kapoor P AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Kyle, Robert A AU - Kyle RA AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Leung, Nelson AU - Leung N AUID- ORCID: 0000-0002-5651-1411 AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. AD - Division of Nephrology, Mayo Clinic, Rochester, Minnesota. FAU - Go, Ronald S AU - Go RS AUID- ORCID: 0000-0002-8284-3495 AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Lust, John A AU - Lust JA AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Rajkumar, S Vincent AU - Rajkumar SV AUID- ORCID: 0000-0002-5862-1833 AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. FAU - Kumar, Shaji K AU - Kumar SK AUID- ORCID: 0000-0001-5392-9284 AD - Division of Hematology, Mayo Clinic, Rochester, Minnesota. LA - eng GR - UL1 TR002377/TR/NCATS NIH HHS/United States GR - NH/NIH HHS/United States PT - Clinical Trial PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20190108 PL - United States TA - Am J Hematol JT - American journal of hematology JID - 7610369 SB - IM MH - Disease-Free Survival MH - Female MH - *Flow Cytometry MH - Humans MH - In Situ Hybridization, Fluorescence MH - *Karyotyping MH - Male MH - Multiple Myeloma/blood/genetics/*mortality/pathology MH - Predictive Value of Tests MH - Survival Rate MH - *Trisomy PMC - PMC8083940 MID - NIHMS1685746 EDAT- 2018/12/29 06:00 MHDA- 2019/12/21 06:00 PMCR- 2021/04/29 CRDT- 2018/12/29 06:00 PHST- 2018/11/26 00:00 [received] PHST- 2018/12/18 00:00 [revised] PHST- 2018/12/26 00:00 [accepted] PHST- 2018/12/29 06:00 [pubmed] PHST- 2019/12/21 06:00 [medline] PHST- 2018/12/29 06:00 [entrez] PHST- 2021/04/29 00:00 [pmc-release] AID - 10.1002/ajh.25391 [doi] PST - ppublish SO - Am J Hematol. 2019 Apr;94(4):424-430. doi: 10.1002/ajh.25391. Epub 2019 Jan 8.