PMID- 30592550
OWN - NLM
STAT- MEDLINE
DCOM- 20191129
LR  - 20191210
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
VI  - 11
IP  - 6
DP  - 2019 Jun
TI  - Development and validation of a liquid chromatography-tandem mass spectrometry 
      method for simultaneous detection of 10 illicit drugs in oral fluid collected 
      with FLOQSwabs and application to real samples.
PG  - 824-832
LID - 10.1002/dta.2563 [doi]
AB  - According to French law, the roadside testing for drugs of abuse (DOA) should be 
      performed in oral fluid (OF) using an immunological screening kit. If the 
      screening is positive, confirmation has to be done in OF collected by a special 
      swab, called the FLOQSwab (FS). Unlike other sampling kits, this device was not 
      designed to collect OF since it does not contain an elution buffer. An analytical 
      method was developed for the simultaneous detection of 10 DOA under control in 
      France: tetrahydrocannabinol (THC) at 1 ng/mL, and cocaine, benzoylecgonine 
      (BZE), morphine, 6-monoacetylmorphine (6-MAM), amphetamine, methamphetamine, 
      3,4-methylenedioxy-N-ethylamphetamine (MDEA), 3,4-methylenedioxyamphetamine 
      (MDA), and 3,4-methylenedioxy-N-methylamphetamine (MDMA) at 10 ng/mL. Samples 
      were eluted using the Quantisal((R)) buffer and extracted by liquid-liquid 
      extraction for THC and by solid-phase extraction for the remaining analytes. 
      Analyses were performed by ultra-high performance liquid chromatography coupled 
      with tandem mass spectrometry (UHPLC-MS/MS). The validated method made it 
      possible to detect the concentrations required by law and was successfully 
      applied to samples from drivers who screened positive. The main limitations of 
      this kit are the large variability of the collected OF volume and the poor 
      stability of DOA in OF, requiring the use of a conservation buffer.
CI  - (c) 2018 John Wiley & Sons, Ltd.
FAU - Fabresse, Nicolas
AU  - Fabresse N
AUID- ORCID: 0000-0001-9131-2306
AD  - Plateforme MasSpecLab, U-INSERM 1173, UFR Simone Veil, Universite de Versailles 
      Saint-Quentin, 2 avenue de la source de la Bievre, 78180, Montigny le Bretonneux, 
      France.
AD  - Laboratoire de Pharmacologie et de Toxicologie, Hopital Raymond Poincare, AP-HP, 
      104 Boulevard Raymond Poincare, 92380, Garches, France.
FAU - Aouad, Hassan
AU  - Aouad H
AD  - Plateforme MasSpecLab, U-INSERM 1173, UFR Simone Veil, Universite de Versailles 
      Saint-Quentin, 2 avenue de la source de la Bievre, 78180, Montigny le Bretonneux, 
      France.
FAU - Knapp, Adeline
AU  - Knapp A
AD  - Laboratoire de Pharmacologie et de Toxicologie, Hopital Raymond Poincare, AP-HP, 
      104 Boulevard Raymond Poincare, 92380, Garches, France.
FAU - Mayer, Charlotte
AU  - Mayer C
AD  - Laboratoire de Pharmacologie et de Toxicologie, Hopital Raymond Poincare, AP-HP, 
      104 Boulevard Raymond Poincare, 92380, Garches, France.
FAU - Etting, Isabelle
AU  - Etting I
AD  - Laboratoire de Pharmacologie et de Toxicologie, Hopital Raymond Poincare, AP-HP, 
      104 Boulevard Raymond Poincare, 92380, Garches, France.
FAU - Larabi, Islam Amine
AU  - Larabi IA
AUID- ORCID: 0000-0002-9015-5342
AD  - Plateforme MasSpecLab, U-INSERM 1173, UFR Simone Veil, Universite de Versailles 
      Saint-Quentin, 2 avenue de la source de la Bievre, 78180, Montigny le Bretonneux, 
      France.
AD  - Laboratoire de Pharmacologie et de Toxicologie, Hopital Raymond Poincare, AP-HP, 
      104 Boulevard Raymond Poincare, 92380, Garches, France.
FAU - Alvarez, Jean-Claude
AU  - Alvarez JC
AD  - Plateforme MasSpecLab, U-INSERM 1173, UFR Simone Veil, Universite de Versailles 
      Saint-Quentin, 2 avenue de la source de la Bievre, 78180, Montigny le Bretonneux, 
      France.
AD  - Laboratoire de Pharmacologie et de Toxicologie, Hopital Raymond Poincare, AP-HP, 
      104 Boulevard Raymond Poincare, 92380, Garches, France.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20190117
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
RN  - 0 (Illicit Drugs)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods
MH  - Humans
MH  - Illicit Drugs/*analysis
MH  - Limit of Detection
MH  - Saliva/*chemistry
MH  - Substance Abuse Detection/*methods
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - FLOQSwab
OT  - drugs of abuse
OT  - oral fluid
OT  - tandem mass spectrometry
EDAT- 2018/12/29 06:00
MHDA- 2019/11/30 06:00
CRDT- 2018/12/29 06:00
PHST- 2018/08/10 00:00 [received]
PHST- 2018/12/19 00:00 [revised]
PHST- 2018/12/20 00:00 [accepted]
PHST- 2018/12/29 06:00 [pubmed]
PHST- 2019/11/30 06:00 [medline]
PHST- 2018/12/29 06:00 [entrez]
AID - 10.1002/dta.2563 [doi]
PST - ppublish
SO  - Drug Test Anal. 2019 Jun;11(6):824-832. doi: 10.1002/dta.2563. Epub 2019 Jan 17.