PMID- 30593359
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20190114
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 228
DP  - 2019 Jan
TI  - Cellular protein profiles altered by PRRSV infection of porcine monocytes-derived 
      dendritic cells.
PG  - 134-142
LID - S0378-1135(18)31001-0 [pii]
LID - 10.1016/j.vetmic.2018.11.016 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells (APCs) that play an 
      important role in inducing primary antigen-specific immune responses. Some 
      viruses have evolved to specifically target DCs to circumvent the host immune 
      responses for their persistence in the host. One example is porcine reproductive 
      and respiratory syndrome virus (PRRSV) that causes a persistent infection in pigs 
      through modulating DC-mediated antiviral response. To study the cellular protein 
      responses in PRRSV-infected monocyte-derived dendritic cells (MoDCs), 
      two-dimensional liquid chromatography-tandem mass spectrometry coupled with 
      isobaric tags for relative and absolute quantification (iTRAQ) labeling was 
      employed to quantitatively identify the differentially expressed proteins in 
      PRRSV-infected MoDCs and the control cells. A total of 252 cellular proteins in 
      MoDCs that were significantly altered at different time periods post-infection 
      were identified. Differentially expressed proteins that are involved in the 
      endocytosis pathway, actin cytoskeleton network, antigen processing and 
      presentation, JAK-STAT signaling pathway and PRRSV receptors were identified and 
      further analyzed. Among them, the expression changes of STAT1, Mx1, PICALM and 
      SLA-DR were further verified by Western blotting. The protein profiles associated 
      with PRRSV infection of MoDCs should offer novel insights to further 
      investigation of PRRSV-mediated antiviral evasion mechanism and its pathobiology 
      in swine.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Hu, Yue
AU  - Hu Y
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Sangyuan Road No. 8, Jinan 250100, China; Biotechnology Research 
      Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
FAU - Wu, Xiangju
AU  - Wu X
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Sangyuan Road No. 8, Jinan 250100, China.
FAU - Feng, Wenhai
AU  - Feng W
AD  - State Key Laboratory of Agrobiotechnology, Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Li, Feng
AU  - Li F
AD  - Department of Biology and Microbiology, Department of Veterinary and Biomedical 
      Sciences, South Dakota State University, Brookings, SD 57007, USA.
FAU - Wang, Zhao
AU  - Wang Z
AD  - Department of Biology and Microbiology, Department of Veterinary and Biomedical 
      Sciences, South Dakota State University, Brookings, SD 57007, USA; China 
      Institute of Veterinary Drug Control, 8 Nandajie, Zhongguancun, Haidian, Beijing 
      100081, China. Electronic address: zhaowang2007@outlook.com.
FAU - Qi, Jing
AU  - Qi J
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Sangyuan Road No. 8, Jinan 250100, China; College of Life Science, 
      Shandong Normal University, Jinan 250014, China. Electronic address: 
      qj-happy2008@163.com.
FAU - Du, Yijun
AU  - Du Y
AD  - Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of 
      Animal Science and Veterinary Medicine, Shandong Academy of Agricultural 
      Sciences, Sangyuan Road No. 8, Jinan 250100, China; College of Veterinary 
      Medicine, Qingdao Agricultural University, Qingdao 266109, China; College of Life 
      Science, Shandong Normal University, Jinan 250014, China. Electronic address: 
      duyijun0916@163.com.
LA  - eng
PT  - Journal Article
DEP - 20181120
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/veterinary
MH  - *Computational Biology
MH  - Dendritic Cells/metabolism/virology
MH  - Monocytes/metabolism/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - *Proteome
MH  - Proteomics
MH  - Swine
MH  - Tandem Mass Spectrometry/veterinary
MH  - Virus Replication
OTO - NOTNLM
OT  - Cellular network
OT  - MoDCs
OT  - PRRSV
OT  - iTRAQ
EDAT- 2018/12/30 06:00
MHDA- 2019/01/15 06:00
CRDT- 2018/12/30 06:00
PHST- 2018/08/27 00:00 [received]
PHST- 2018/11/14 00:00 [revised]
PHST- 2018/11/16 00:00 [accepted]
PHST- 2018/12/30 06:00 [entrez]
PHST- 2018/12/30 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
AID - S0378-1135(18)31001-0 [pii]
AID - 10.1016/j.vetmic.2018.11.016 [doi]
PST - ppublish
SO  - Vet Microbiol. 2019 Jan;228:134-142. doi: 10.1016/j.vetmic.2018.11.016. Epub 2018 
      Nov 20.