PMID- 30593815
OWN - NLM
STAT- MEDLINE
DCOM- 20190531
LR  - 20190531
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 126
DP  - 2019 Apr 1
TI  - Ultrasonic-assisted enzymatic extraction and characterization of polysaccharides 
      from dandelion (Taraxacum officinale) leaves.
PG  - 846-856
LID - S0141-8130(18)34764-0 [pii]
LID - 10.1016/j.ijbiomac.2018.12.232 [doi]
AB  - Ultrasonic-assisted enzymatic extraction (UAEE) technology was employed to 
      extract the polysaccharides from dandelion leaves (DLP). The extraction 
      conditions were optimized by response surface method (RSM) and Box-Behnken design 
      (BBD). The polysaccharide yield (14.05 +/- 0.95%), which well matched with the 
      predicted value of 14.27%, was obtained under the optimum parameters: ultrasound 
      power 500 W, ultrasound time 42 min, cellulase amount 4.90%, and particle size 
      80 mesh. After purification by AB-8 Macroporous Resin and Sephadex G-100 column, 
      a novel polysaccharide (DLP-I) was isolated and its molecular weight was 
      estimated to be 87,000 g/mol by high performance liquid chromatography (HPLC). 
      Gas chromatography (GC) analysis showed that DLP-I was composed of rhamnose, 
      arabinose, mannose, galactose and glucose in the molar ratio of 
      1.14:1.00:1.05:4.76:1.52. 1D and 2D NMR spectra confirmed that the backbone of 
      DLP-I was mainly consisted of -->4)-alpha-D-Galp-(1-->, -->4)-beta-D-Manp-(1-->, 
      -->4)-alpha-D-Glcp-(1-->, -->2,4)-alpha-L-Rhap-(1-->, and alpha-L-Araf-(1--> with branching at O-2 and 
      O-4 of -->2,4)-alpha-L-Rhap-(1-->. In addition, the digestion results showed DLP-I could 
      keep its stability during the simulated digestive juices.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Wang, Libo
AU  - Wang L
AD  - College of Science, Northeast Agricultural University, Harbin 150030, People's 
      Republic of China.
FAU - Li, Tengfei
AU  - Li T
AD  - College of Science, Northeast Agricultural University, Harbin 150030, People's 
      Republic of China.
FAU - Liu, Fangcheng
AU  - Liu F
AD  - College of Science, Northeast Agricultural University, Harbin 150030, People's 
      Republic of China.
FAU - Liu, Dawei
AU  - Liu D
AD  - College of Agriculture, Northeast Agricultural University, Harbin 150030, 
      People's Republic of China. Electronic address: liudawei@neau.edu.cn.
FAU - Xu, Yaqin
AU  - Xu Y
AD  - College of Science, Northeast Agricultural University, Harbin 150030, People's 
      Republic of China.
FAU - Yang, Yu
AU  - Yang Y
AD  - College of Science, Northeast Agricultural University, Harbin 150030, People's 
      Republic of China.
FAU - Zhao, Yanbo
AU  - Zhao Y
AD  - College of Science, Northeast Agricultural University, Harbin 150030, People's 
      Republic of China.
FAU - Wei, He
AU  - Wei H
AD  - College of Science, Northeast Agricultural University, Harbin 150030, People's 
      Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20181226
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Analysis of Variance
MH  - Magnetic Resonance Spectroscopy
MH  - Particle Size
MH  - Plant Leaves/*chemistry
MH  - Polysaccharides/chemistry/*isolation & purification
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Taraxacum/*chemistry
MH  - Time Factors
MH  - Ultrasonics/*methods
OTO - NOTNLM
OT  - Dandelion
OT  - Polysaccharides
OT  - Simulated digestion
OT  - Structural characterization
OT  - Ultrasonic-assisted enzymatic extraction
EDAT- 2018/12/30 06:00
MHDA- 2019/06/01 06:00
CRDT- 2018/12/30 06:00
PHST- 2018/09/07 00:00 [received]
PHST- 2018/12/18 00:00 [revised]
PHST- 2018/12/23 00:00 [accepted]
PHST- 2018/12/30 06:00 [pubmed]
PHST- 2019/06/01 06:00 [medline]
PHST- 2018/12/30 06:00 [entrez]
AID - S0141-8130(18)34764-0 [pii]
AID - 10.1016/j.ijbiomac.2018.12.232 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2019 Apr 1;126:846-856. doi: 10.1016/j.ijbiomac.2018.12.232. 
      Epub 2018 Dec 26.