PMID- 30596395
OWN - NLM
STAT- MEDLINE
DCOM- 20190806
LR  - 20190806
IS  - 1309-5730 (Electronic)
IS  - 1018-5615 (Linking)
VI  - 35
IP  - 1
DP  - 2019
TI  - Detection of Anaplastic Lymphoma Kinase Gene Re-Arrangement in Non-Small Cell 
      Lung Carcinoma in the Indian Population: Comparison of Techniques and 
      Immunohistochemistry Clones.
PG  - 36-45
LID - 10.5146/tjpath.2018.01438 [doi]
AB  - OBJECTIVE: Predictive and prognostic markers have revolutionized personalized 
      therapy in non-small cell lung carcinoma (NSCLC). Crizotinib is now approved for 
      locally advanced or metastatic NSCLC that is anaplastic lymphoma kinase (ALK) 
      positive by either Fluorescence in situ hybridization (FISH) or 
      immunohistochemistry (IHC). The current study aimed to detect the incidence of 
      ALK gene re-arrangement in the Indian population, to compare the various IHC 
      antibodies with FISH as a gold standard, and to analyze the morphology of cases 
      with ALK phenotype. MATERIAL AND METHOD: A case series of 614 cases of NSCLC were 
      included. IHC for detection of ALK phenotype was compared with FISH using 5A4 
      clone (Labvision, USA), ALK-1(Dako, Denmark) and D5F3 clone (Ventana, USA). 
      RESULTS: ALK gene rearrangement was evident in 4.07% of the cases. Cases with ALK 
      phenotype had unique histomorphology with presence of mucin or signet ring cells 
      in association with necrosis, high tumour grade and poor differentiation. 
      Comparison of various antibody clones used in IHC revealed that the sensitivity 
      and specificity using the D5F3 clone (100%, 100%) and 5A4 clone (87.5%, 100%) 
      were similar while the ALK-1 clone had the lowest sensitivity and specificity 
      (50%, 95.5%). CONCLUSION: The incidence of ALK gene rearrangement in NSCLC in the 
      current Indian study is within the worldwide reported range of 3-5%. This is the 
      first study from the Indian subcontinent to compare various IHC antibodies used 
      for detection of ALK phenotype. IHC using D5F3 clone and 5A4 clone may be 
      considered as a rapid reliable and inexpensive method for detection of ALK gene 
      rearrangement.
FAU - Shukla, Saumya
AU  - Shukla S
AD  - Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, 
      LUCKNOW, UP-INDIA.
FAU - Pandey, Rahul Kumar
AU  - Pandey RK
FAU - Kant, Surya
AU  - Kant S
FAU - Garg, Rajiv
AU  - Garg R
FAU - Anand, Nidhi
AU  - Anand N
FAU - Kushwaha, Ram
AU  - Kushwaha R
FAU - Malhotra, Kiran Preet
AU  - Malhotra KP
FAU - Srivastava, Anand
AU  - Srivastava A
FAU - Bhatt, Madan
AU  - Bhatt M
FAU - Husain, Nuzhat
AU  - Husain N
LA  - eng
PT  - Comparative Study
PT  - Journal Article
TT  - Detection of Anaplastic Lymphoma Kinase Gene Re-Arrangement in Non-Small Cell 
      Lung Carcinoma in the Indian Population: Comparison of Techniques and 
      Immunohistochemistry Clones.
PL  - Turkey
TA  - Turk Patoloji Derg
JT  - Turk patoloji dergisi
JID - 9440471
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
MH  - Adult
MH  - Age Distribution
MH  - Aged
MH  - Aged, 80 and over
MH  - Anaplastic Lymphoma Kinase/*genetics/metabolism
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Female
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - India
MH  - Lung Neoplasms/*genetics/pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Necrosis
MH  - Sensitivity and Specificity
MH  - Sex Distribution
MH  - Smoking/genetics
MH  - Tertiary Care Centers
MH  - Young Adult
EDAT- 2019/01/01 06:00
MHDA- 2019/08/07 06:00
CRDT- 2019/01/01 06:00
PHST- 2019/01/01 06:00 [pubmed]
PHST- 2019/08/07 06:00 [medline]
PHST- 2019/01/01 06:00 [entrez]
AID - 10.5146/tjpath.2018.01438 [doi]
PST - ppublish
SO  - Turk Patoloji Derg. 2019;35(1):36-45. doi: 10.5146/tjpath.2018.01438.