PMID- 30597574
OWN - NLM
STAT- MEDLINE
DCOM- 20191023
LR  - 20200108
IS  - 1531-4995 (Electronic)
IS  - 0023-852X (Linking)
VI  - 129
IP  - 9
DP  - 2019 Sep
TI  - Tra2beta silencing suppresses cell proliferation in laryngeal squamous cell 
      carcinoma via inhibiting PI3K/AKT signaling.
PG  - E318-E328
LID - 10.1002/lary.27716 [doi]
AB  - OBJECTIVES/HYPOTHESIS: Transformer-2 protein homolog beta (Tra2beta) generally plays 
      an important role in various human cancers, but its role and the underlying 
      mechanisms in laryngeal squamous cell carcinoma (LSCC) remained unknown. So this 
      study aimed to assess the clinical significance and regulatory mechanisms of 
      Tra2beta in LSCC. STUDY DESIGN: Laboratory analysis. METHODS: Expression of Tra2beta 
      was compared in human LSCC tissue samples and paired adjacent normal tissue 
      samples. The in vitro effects of Tra2beta expression in Hep-2 cells on their 
      proliferation, invasion, and migration were assessed by CCK-8 assays, Matrigel 
      invasion, and transwell migration assays. In addition, the effects of 
      downregulation of Tra2beta on the activation of PI3K/AKT signaling pathway were 
      measured using Western blot analysis. The effect of Tra2beta on the growth of tumors 
      was detected in the Hep-2-injected xenograft models in vivo. RESULTS: 
      Reverse-transcription quantitative polymerase chain reaction analysis and 
      immunochemistry analysis indicated that the increased expression of Tra2beta in LSCC 
      was significantly associated with poor differentiation, lymph node metastasis, 
      and advanced clinical stage. In vitro knockdown of Tra2beta caused a significant 
      decrease in the proliferation, invasion, and migration of Hep-2 cells. Tra2beta 
      silencing decreased the expression of Bcl-2 but increased Bax and Caspase-3 both 
      in mRNA and protein levels. Furthermore, knockdown of Tra2beta eliminated the 
      suppressive effects of activation of PI3K/AKT signaling. In vivo knockdown of 
      Tra2beta significantly inhibited the tumor growth of Hep-2-injected xenograft mice. 
      CONCLUSIONS: The results of the present study demonstrated that knockdown of 
      Tra2beta inhibits the proliferation and invasion of LSCC cells, at least partly via 
      inhibiting PI3K/AKT signaling. LEVEL OF EVIDENCE: NA Laryngoscope, 129:E318-E328, 
      2019.
CI  - (c) 2018 The American Laryngological, Rhinological and Otological Society, Inc.
FAU - Ni, Hao-Sheng
AU  - Ni HS
AUID- ORCID: 0000-0002-8491-6707
AD  - Department of Otorhinolaryngology, First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
AD  - Department of Otorhinolaryngology, Affiliated Hospital of Nantong University, 
      Nantong, China.
FAU - Hu, Song-Qun
AU  - Hu SQ
AD  - Department of Otorhinolaryngology, First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
AD  - Department of Otorhinolaryngology, Affiliated Hospital of Nantong University, 
      Nantong, China.
FAU - Chen, Xi
AU  - Chen X
AD  - Department of Otorhinolaryngology, First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
FAU - Liu, Yi-Fei
AU  - Liu YF
AD  - Department of Pathology, Affiliated Hospital of Nantong University, Nantong, 
      China.
FAU - Ni, Ting-Ting
AU  - Ni TT
AD  - Department of Oncology, Nantong Tumor Hospital, Nantong, China.
FAU - Cheng, Lei
AU  - Cheng L
AD  - Department of Otorhinolaryngology, First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181231
PL  - United States
TA  - Laryngoscope
JT  - The Laryngoscope
JID - 8607378
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (TRA2B protein, human)
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Silencing/*physiology
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Nerve Tissue Proteins/*metabolism
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Serine-Arginine Splicing Factors/*metabolism
MH  - Signal Transduction/*genetics
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma
OT  - PI3K/AKT signaling pathway
OT  - cell migration
OT  - cell proliferation
OT  - transformer-2 protein homology beta
EDAT- 2019/01/01 06:00
MHDA- 2019/10/24 06:00
CRDT- 2019/01/01 06:00
PHST- 2018/10/26 00:00 [accepted]
PHST- 2019/01/01 06:00 [pubmed]
PHST- 2019/10/24 06:00 [medline]
PHST- 2019/01/01 06:00 [entrez]
AID - 10.1002/lary.27716 [doi]
PST - ppublish
SO  - Laryngoscope. 2019 Sep;129(9):E318-E328. doi: 10.1002/lary.27716. Epub 2018 Dec 
      31.