PMID- 30597721
OWN - NLM
STAT- MEDLINE
DCOM- 20200714
LR  - 20200714
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 107
IP  - 6
DP  - 2019 Jun
TI  - TEGDMA releasing in resin composites with different filler contents and its 
      correlation with mitochondrial mediated cytotoxicity in human gingival 
      fibroblasts.
PG  - 1132-1142
LID - 10.1002/jbm.a.36600 [doi]
AB  - Compositions of resin composite exhibit cytotoxicity, especially 
      Triethylene-glycol-dimethacrylate (TEGDMA), yet the underlying mechanisms and its 
      relationship with filler content are poorly understood. Here, specimens of five 
      composites (VITA LC, VITA ZETA, Z350, Filtek P60, and AP-X), containing different 
      filler size and weight, were immersed into culture medium for 72 h. After TEGDMA 
      quantification, the resin composite eluates were used to incubate HGFs. Cellular 
      viability was evaluated. Total reactive oxygen species (ROS) and mitochondrial 
      ROS were detected to assess oxidative stress. Adenosine triphosphate and 
      cytochrome c oxidase (CcO) activity, mitochondrial membrane potential and 
      morphology, mitochondrial biogenesis regulators were analyzed to evaluate 
      mitochondrial functions. Results showed that TEGDMA release negatively correlated 
      to filler size and weight of tested composites. Although cell viability reduction 
      was not significant, total and mitochondrial ROS production showed a positive 
      relationship with the amount of TEGDMA in composite eluates. Furthermore, the 
      expression of mitochondrial biogenesis markers and mitochondrial fusion protein, 
      were markedly elevated in TEGDMA rich eluates, especially in VITA-LC group, shown 
      as elongated mitochondrial morphology and aberrant mitochondrial functions. 
      Overall, TEGDMA could elute easier from those resin composites with less filler 
      content and cause oxidative stress in HGFs via mitochondria dysregulation. These 
      data can be instructive to optimize the synthesis of resin composites from the 
      perspective of biocompatibility. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater 
      Res Part A: 107A: 1132-1142, 2019.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Liu, Beilei
AU  - Liu B
AD  - State Key Laboratory of Oral Disease, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, 
      PR China.
FAU - Gan, Xueqi
AU  - Gan X
AD  - State Key Laboratory of Oral Disease, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, 
      PR China.
FAU - Zhao, Yuwei
AU  - Zhao Y
AD  - State Key Laboratory of Oral Disease, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, 
      PR China.
FAU - Chen, Junsheng
AU  - Chen J
AD  - State Key Laboratory of Oral Disease, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, 
      PR China.
FAU - Yu, Hongdou
AU  - Yu H
AD  - State Key Laboratory of Oral Disease, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, 
      PR China.
FAU - Gao, Jing
AU  - Gao J
AD  - State Key Laboratory of Oral Disease, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, 
      PR China.
FAU - Yu, Haiyang
AU  - Yu H
AD  - State Key Laboratory of Oral Disease, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, 
      PR China.
LA  - eng
GR  - 2016YFC1102704/National Key Research and Development Program of 
      China/International
GR  - 81870802/National Natural Science Foundation of China/International
GR  - CSA-R2018-05 (G. XQ)/Research Fund for Resin Materials of Chinese Stomatological 
      Association/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190305
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Composite Resins)
RN  - 0 (Polymethacrylic Acids)
RN  - 14I47YJ5EY (triethylene glycol dimethacrylate)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - *Composite Resins/adverse effects/chemistry/pharmacology
MH  - Fibroblasts/*metabolism/pathology
MH  - Gingiva/*metabolism/pathology
MH  - Humans
MH  - Materials Testing
MH  - Mitochondria/*metabolism/pathology
MH  - *Polyethylene Glycols/adverse effects/chemistry/pharmacology
MH  - *Polymethacrylic Acids/adverse effects/chemistry/pharmacology
OTO - NOTNLM
OT  - TEGDMA
OT  - cytotoxicity
OT  - mitochondria
OT  - oxidative stress
OT  - resin composite
EDAT- 2019/01/01 06:00
MHDA- 2020/07/15 06:00
CRDT- 2019/01/01 06:00
PHST- 2018/09/17 00:00 [received]
PHST- 2018/11/27 00:00 [revised]
PHST- 2018/12/26 00:00 [accepted]
PHST- 2019/01/01 06:00 [pubmed]
PHST- 2020/07/15 06:00 [medline]
PHST- 2019/01/01 06:00 [entrez]
AID - 10.1002/jbm.a.36600 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2019 Jun;107(6):1132-1142. doi: 10.1002/jbm.a.36600. Epub 
      2019 Mar 5.