PMID- 30598727 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 1993-0771 (Print) IS - 1993-078X (Electronic) IS - 1993-0771 (Linking) VI - 12 IP - 4 DP - 2018 TI - New insights into the karyotype evolution of the genus Gampsocleis (Orthoptera, Tettigoniinae, Gampsocleidini). PG - 529-538 LID - 10.3897/CompCytogen.v12i4.29574 [doi] AB - Five species belonging to the genus Gampsocleis Fieber, 1852 were analyzed using fluorescence in situ hybridization (FISH) with 18S rDNA and telomeric probes, as well as C-banding, DAPI/CMA(3) staining and silver impregnation. The studied species showed two distinct karyotypes, with 2n = 31 (male) and 2n = 23 (male) chromosomes. The drastic reduction in chromosome number observed in the latter case suggests multiple translocations and fusions as the main responsible that occurred during chromosome evolution. Two groups of rDNA distribution were found in Gampsocleis representatives analyzed. Group 1, with a single large rDNA cluster on the medium-sized autosome found in four species, carried in the haploid karyotype. Group 2, represented only by G.abbreviata, was characterized by the presence of two rDNA signals. TTAGG telomeric repeats were found at the ends of chromosome arms as expected. The rDNA clusters coincided with active NORs and GC-rich segments. FAU - Kocinski, Maciej AU - Kocinski M AUID- ORCID: 0000-0002-6601-489X AD - Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska 17, 31-016 Krakow, Poland Institute of Systematics and Evolution of Animals, Polish Academy of Sciences Krakow Poland. FAU - Grzywacz, Beata AU - Grzywacz B AD - Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska 17, 31-016 Krakow, Poland Institute of Systematics and Evolution of Animals, Polish Academy of Sciences Krakow Poland. FAU - Chobanov, Dragan AU - Chobanov D AD - Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 1 Tsar Osvoboditel Boul., 1000 Sofia, Bulgaria Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences Sofia Bulgaria. FAU - Archalowska-Sliwa, Elzbieta AU - Archalowska-Sliwa E AD - Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Slawkowska 17, 31-016 Krakow, Poland Institute of Systematics and Evolution of Animals, Polish Academy of Sciences Krakow Poland. LA - eng PT - Journal Article DEP - 20181219 PL - Bulgaria TA - Comp Cytogenet JT - Comparative cytogenetics JID - 101503807 PMC - PMC6306472 OTO - NOTNLM OT - Gampsocleis OT - Orthoptera OT - 18S rDNA OT - Ag-NOR OT - FISH OT - chromosome evolution OT - fluorochrome staining OT - telomeric repeats EDAT- 2019/01/02 06:00 MHDA- 2019/01/02 06:01 PMCR- 2018/12/19 CRDT- 2019/01/02 06:00 PHST- 2018/09/06 00:00 [received] PHST- 2018/12/06 00:00 [accepted] PHST- 2019/01/02 06:00 [entrez] PHST- 2019/01/02 06:00 [pubmed] PHST- 2019/01/02 06:01 [medline] PHST- 2018/12/19 00:00 [pmc-release] AID - 10.3897/CompCytogen.v12i4.29574 [doi] PST - epublish SO - Comp Cytogenet. 2018 Dec 19;12(4):529-538. doi: 10.3897/CompCytogen.v12i4.29574. eCollection 2018.