PMID- 30599454
OWN - NLM
STAT- MEDLINE
DCOM- 20190326
LR  - 20190326
IS  - 1879-3460 (Electronic)
IS  - 0168-1605 (Linking)
VI  - 292
DP  - 2019 Mar 2
TI  - Persistent viremia and presence of hepatitis E virus RNA in pig muscle meat after 
      experimental co-infection with porcine reproductive and respiratory syndrome 
      virus.
PG  - 144-149
LID - S0168-1605(18)30937-1 [pii]
LID - 10.1016/j.ijfoodmicro.2018.12.023 [doi]
AB  - Although hepatitis E virus (HEV) transmission has been demonstrated after 
      consumption of products containing infected pig liver, human cases can be also 
      associated with other pig meat products, such as sausages. Data on HEV viremia 
      and dissemination in muscle meat of infected animals are still sparse, especially 
      during long-term infection. Previously, we have shown that experimental 
      co-infection of pigs with HEV and porcine reproductive and respiratory syndrome 
      virus (PRRSV) lengthens HEV infection up to 49 days and increases the likelihood 
      of the presence of HEV RNA in the liver of the pig at a later stage of infection. 
      In the present study, we show that during experimental HEV-PRRSV co-infection, 
      prolonged HEV viremia, up to 49 days post-inoculation (dpi), is detected. The 
      long-term viremia observed was statistically associated with the absence of HEV 
      seroconversion. HEV RNA was also frequently detected, at a late stage of 
      infection (49 dpi), in the three different types of muscle tested: femoral 
      biceps, psoas major or diaphragm pillar. The HEV RNA load could reach up to 
      1 . 10(6) genome copies per gram of muscle. Detection of HEV in muscle meat was 
      statistically associated with high HEV loads in corresponding liver and fecal 
      samples. The presence of HEV in pig blood, femoral biceps and major psoas, 
      corresponding to ham and tenderloin muscles respectively, is of concern for the 
      food industry. Hence, these results indicate new potential risks for consumers 
      and public health regarding pork products.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Salines, Morgane
AU  - Salines M
AD  - ANSES, French Agency for Food, Environmental and Occupational Health & Safety, 
      Ploufragan/Plouzane Laboratory, BP53, 22440 Ploufragan, France; Bretagne-Loire 
      University, Rennes, France. Electronic address: morgane.salines@anses.fr.
FAU - Demange, Antonin
AU  - Demange A
AD  - ANSES, Laboratoire de Sante Animale, UMR 1161 Virology, 94706 Maisons-Alfort, 
      France; INRA, UMR 1161 Virology, 94706 Maisons-Alfort, France; Ecole Nationale 
      Veterinaire d'Alfort, UMR 1161 Virology, 94706 Maisons-Alfort, France.
FAU - Stephant, Gael
AU  - Stephant G
AD  - ANSES, Laboratoire de Sante Animale, UMR 1161 Virology, 94706 Maisons-Alfort, 
      France; INRA, UMR 1161 Virology, 94706 Maisons-Alfort, France; Ecole Nationale 
      Veterinaire d'Alfort, UMR 1161 Virology, 94706 Maisons-Alfort, France.
FAU - Renson, Patricia
AU  - Renson P
AD  - ANSES, French Agency for Food, Environmental and Occupational Health & Safety, 
      Ploufragan/Plouzane Laboratory, BP53, 22440 Ploufragan, France; Bretagne-Loire 
      University, Rennes, France; Union des Groupements de Producteurs de Viande de 
      Bretagne (UGPVB), 104 rue Eugene Pottier, 35065 Rennes, cedex, France. Electronic 
      address: patricia.renson@anses.fr.
FAU - Bourry, Olivier
AU  - Bourry O
AD  - ANSES, French Agency for Food, Environmental and Occupational Health & Safety, 
      Ploufragan/Plouzane Laboratory, BP53, 22440 Ploufragan, France; Bretagne-Loire 
      University, Rennes, France. Electronic address: olivier.bourry@anses.fr.
FAU - Andraud, Mathieu
AU  - Andraud M
AD  - ANSES, French Agency for Food, Environmental and Occupational Health & Safety, 
      Ploufragan/Plouzane Laboratory, BP53, 22440 Ploufragan, France; Bretagne-Loire 
      University, Rennes, France. Electronic address: mathieu.andraud@anses.fr.
FAU - Rose, Nicolas
AU  - Rose N
AD  - ANSES, French Agency for Food, Environmental and Occupational Health & Safety, 
      Ploufragan/Plouzane Laboratory, BP53, 22440 Ploufragan, France; Bretagne-Loire 
      University, Rennes, France. Electronic address: nicolas.rose@anses.fr.
FAU - Pavio, Nicole
AU  - Pavio N
AD  - ANSES, Laboratoire de Sante Animale, UMR 1161 Virology, 94706 Maisons-Alfort, 
      France; INRA, UMR 1161 Virology, 94706 Maisons-Alfort, France; Ecole Nationale 
      Veterinaire d'Alfort, UMR 1161 Virology, 94706 Maisons-Alfort, France. Electronic 
      address: nicole.pavio@anses.fr.
LA  - eng
PT  - Journal Article
DEP - 20181226
PL  - Netherlands
TA  - Int J Food Microbiol
JT  - International journal of food microbiology
JID - 8412849
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Coinfection/diagnosis/*virology
MH  - Feces/virology
MH  - *Food Contamination
MH  - Food Microbiology
MH  - Hepatitis E/diagnosis/transmission
MH  - Hepatitis E virus/*isolation & purification
MH  - Meat Products/virology
MH  - Muscle, Skeletal/*virology
MH  - Porcine Reproductive and Respiratory Syndrome/diagnosis/transmission
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - RNA, Viral/isolation & purification
MH  - Red Meat/*virology
MH  - Risk Factors
MH  - Swine/virology
MH  - Swine Diseases/virology
MH  - Viremia/*diagnosis
OTO - NOTNLM
OT  - Hepatitis E virus
OT  - Immunomodulatory co-infection
OT  - Pig meat
OT  - Public health risk
OT  - Viremia
EDAT- 2019/01/02 06:00
MHDA- 2019/03/27 06:00
CRDT- 2019/01/02 06:00
PHST- 2018/06/14 00:00 [received]
PHST- 2018/12/05 00:00 [revised]
PHST- 2018/12/22 00:00 [accepted]
PHST- 2019/01/02 06:00 [pubmed]
PHST- 2019/03/27 06:00 [medline]
PHST- 2019/01/02 06:00 [entrez]
AID - S0168-1605(18)30937-1 [pii]
AID - 10.1016/j.ijfoodmicro.2018.12.023 [doi]
PST - ppublish
SO  - Int J Food Microbiol. 2019 Mar 2;292:144-149. doi: 
      10.1016/j.ijfoodmicro.2018.12.023. Epub 2018 Dec 26.