PMID- 30600669 OWN - NLM STAT- MEDLINE DCOM- 20190121 LR - 20210915 IS - 1002-1892 (Print) IS - 1002-1892 (Linking) VI - 32 IP - 10 DP - 2018 Oct 15 TI - [Study on the protective mechanism of autophagy on cartilage by magnesium sulfate]. PG - 1340-1345 LID - 10.7507/1002-1892.201804015 [doi] AB - OBJECTIVE: To investigate the mechanism of magnesium sulfate in protecting rabbit cartilage by initiating autophagy. METHODS: Twenty-four adult female New Zealand rabbits were used to prepare post-traumatic osteoarthritis (PTOA) models by anterior cruciate ligament transection. Then, the PTOA models were randomly divided into PTOA group, distilled water group, and magnesium sulfate group, with 8 rabbits in each group. Immediately after operation, the distilled water group and the magnesium sulfate group were injected with 0.5 mL distilled water and 20 mmol/L magnesium sulfate solution in the joint cavity 3 times a week for 4 weeks, respectively. The PTOA group was not treated. The general condition of the animals was observed after operation. After 4 weeks, the expressions of tumor necrosis factor alpha (TNF-alpha) and collagen typeⅡ in the joint fluid and the expression of collagen type Ⅱ in venous blood were detected by ELISA assay. The protein expressions of transient receptor potential channel vanilloid 5 (TRPV5) and microtubule associated protein 1 light chain 3 (LC3; LC3-Ⅱ/LC3-Ⅰ) in femoral cartilage were detected by Western blot. The mRNA expressions of interleukin 1beta (IL-1beta), TNF-alpha, matrix metalloproteinases 3 (MMP-3) in synovial tissue and collagen type Ⅱ, Aggrecan (AGN), SOX9 in cartilage tissue were detected by real-time fluorescence quantitative PCR. Cartilage tissue sections were stained with HE staining, Masson staining, and Alcian blue staining and scored according to the modified histological osteoarthritis (OA) score. RESULTS: All animals survived until the experiment was completed. Compared with the other two groups, the expression of TNF-alpha in joint effusion and collagen type Ⅱ in joint effusion and venous blood were decreased in magnesium sulfate group; the protein expression of TRPV5 decreased, and the ratio of LC3-Ⅱ/LC3-Ⅰ increased significantly; the mRNA expressions of IL-1beta, TNF-alpha, and MMP-3 in synovial tissue were decreased, and the mRNA expressions of collagen type Ⅱ, AGN, and SOX9 in cartilage tissue were increased; OA scores also decreased significantly. All differences were statistically significant ( P<0.05). There was no significant difference in the above indicators between the PTOA group and the distilled water group ( P>0.05). CONCLUSION: Intra-articular injection of magnesium sulfate can reduce intra-articular inflammation, reduce the loss of collagen type Ⅱ and AGN, and is beneficial to cartilage regeneration in rabbits. The mechanism may be related to the initiation of chondroautophagy by inhibiting the calcium channel TRPV5. FAU - Chen, Rong AU - Chen R AD - Department of Traumatic Orthopedics, the People's Hospital of Shiyan, Affiliated to Hubei University of Medicine, Shiyan Hubei, 442000, P.R.China. FAU - Zhou, Xue AU - Zhou X AD - The Fourth Hospital of Clinical Medicine, Hubei University of Medicine, Shiyan Hubei, 442000, P.R.China. FAU - Yin, Shaomei AU - Yin S AD - The Fourth Hospital of Clinical Medicine, Hubei University of Medicine, Shiyan Hubei, 442000, P.R.China. FAU - Lu, Zeyu AU - Lu Z AD - The Fourth Hospital of Clinical Medicine, Hubei University of Medicine, Shiyan Hubei, 442000, P.R.China. FAU - Nie, Jinpeng AU - Nie J AD - The Fourth Hospital of Clinical Medicine, Hubei University of Medicine, Shiyan Hubei, 442000, P.R.China. FAU - Zhou, Wencheng AU - Zhou W AD - The Fourth Hospital of Clinical Medicine, Hubei University of Medicine, Shiyan Hubei, 442000, P.R.China. FAU - Liu, Xinghui AU - Liu X AD - Department of Anatomy, Hubei University of Medicine, Shiyan Hubei, 442000, P.R.China.liuxinghui555@163.com. LA - chi PT - Journal Article PL - China TA - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi JT - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JID - 9425194 RN - 0 (Aggrecans) RN - 0 (Collagen Type II) RN - 0 (Interleukin-1beta) RN - 0 (Tumor Necrosis Factor-alpha) RN - 7487-88-9 (Magnesium Sulfate) SB - IM MH - Aggrecans/*drug effects MH - Animals MH - Anterior Cruciate Ligament/*drug effects MH - *Autophagy MH - Cartilage, Articular/*drug effects MH - Collagen Type II/*drug effects MH - Disease Models, Animal MH - Female MH - Injections, Intra-Articular MH - Interleukin-1beta/metabolism MH - Magnesium Sulfate/administration & dosage/*pharmacology MH - Osteoarthritis/*drug therapy MH - Rabbits MH - Synovial Fluid MH - Synovial Membrane MH - Tumor Necrosis Factor-alpha/metabolism PMC - PMC8414159 OTO - NOTNLM OT - Post-traumatic osteoarthritis OT - autophagy OT - cartilage injury OT - magnesium sulfate OT - rabbit EDAT- 2019/01/03 06:00 MHDA- 2019/01/22 06:00 PMCR- 2018/10/01 CRDT- 2019/01/03 06:00 PHST- 2019/01/03 06:00 [entrez] PHST- 2019/01/03 06:00 [pubmed] PHST- 2019/01/22 06:00 [medline] PHST- 2018/10/01 00:00 [pmc-release] AID - zgxfcjwkzz-32-10-1340 [pii] AID - 10.7507/1002-1892.201804015 [doi] PST - ppublish SO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Oct 15;32(10):1340-1345. doi: 10.7507/1002-1892.201804015.