PMID- 30603552
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200225
IS  - 2212-5469 (Electronic)
IS  - 1738-2696 (Print)
IS  - 1738-2696 (Linking)
VI  - 15
IP  - 3
DP  - 2018 Jun
TI  - In Vitro Anti-Inflammation and Chondrogenic Differentiation Effects of Inclusion 
      Nanocomplexes of Hyaluronic Acid-Beta Cyclodextrin and Simvastatin.
PG  - 263-274
LID - 10.1007/s13770-018-0119-9 [doi]
AB  - The aim of this study was to prepare inclusion nanocomplexes of hyaluronic 
      acid-beta-cyclodextrin and simvastatin (HA-beta-CD/SIM) and evaluate in vitro 
      anti-inflammation effects on lipopolysaccharide (LPS)-activated synoviocytes and 
      chondrogenic differentiation effects on rat adipose-derived stem cells (rADSCs). 
      The beta-CD moieties in HA-beta-CD could incorporate SIM to form HA-beta-CD/SIM 
      nanocomplexes with diameters of 297-350 nm. HA-beta-CD/SIM resulted in long-term 
      release of SIM from the nanocomplexes for up to 63 days in a sustained manner. In 
      vitro studies revealed that HA-beta-CD/SIM nanocomplexes were able to effectively 
      and dose-dependently suppress the mRNA expression levels of pro-inflammatory 
      markers such as matrix metallopeptidase-3 (MMP-3), MMP-13, cyclooxygenase-2 
      (COX-2), a disintegrin and metalloproteinase with thrombospondin motifs-5 
      (ADAMTS-5), interleukin-6 (IL-6), and tumor necrosis factor (TNF-alpha) in 
      LPS-stimulated synoviocytes. HA-beta-CD/SIM-treated rADSCs significantly and 
      dose-dependently enhanced mRNA expressions of aggrecan, collagen type II 
      (COL2A1), and collagen type X (COL10A1), implying that HA-beta-CD/SIM greatly 
      induced the chondrogenic differentiation of rADSCs. Conclusively, HA-beta-CD/SIM 
      nanocomplexes will be a promising therapeutic material to alleviate inflammation 
      as well as promote chondrogenesis.
FAU - Kim, Tae-Hoon
AU  - Kim TH
AD  - 1Department of Orthopedic Surgery, Konkuk University School of Medicine, 120-1 
      Neungdong-ro, Hwayang-dong, Gwangjin-gu, Seoul, 05029 Korea. ISNI: 0000 0004 0532 
      8339. GRID: grid.258676.8
FAU - Yun, Young-Pil
AU  - Yun YP
AD  - 2Department of Orthopedic Surgery and Rare Diseases Institute, Korea University 
      Guro Hospital, Korea University College of Medicine, #148, Guro-dong, Guro-gu, 
      Seoul, 08308 Korea. ISNI: 0000 0001 0840 2678. GRID: grid.222754.4
FAU - Shim, Kyu-Sik
AU  - Shim KS
AD  - 2Department of Orthopedic Surgery and Rare Diseases Institute, Korea University 
      Guro Hospital, Korea University College of Medicine, #148, Guro-dong, Guro-gu, 
      Seoul, 08308 Korea. ISNI: 0000 0001 0840 2678. GRID: grid.222754.4
AD  - 3Department of Biomedical Science, Korea University College of Medicine, Korea 
      University, Anam-dong, Seongbuk-gu, Seoul, 02841 Korea. ISNI: 0000 0001 0840 
      2678. GRID: grid.222754.4
FAU - Kim, Hak-Jun
AU  - Kim HJ
AD  - 2Department of Orthopedic Surgery and Rare Diseases Institute, Korea University 
      Guro Hospital, Korea University College of Medicine, #148, Guro-dong, Guro-gu, 
      Seoul, 08308 Korea. ISNI: 0000 0001 0840 2678. GRID: grid.222754.4
FAU - Kim, Sung Eun
AU  - Kim SE
AD  - 2Department of Orthopedic Surgery and Rare Diseases Institute, Korea University 
      Guro Hospital, Korea University College of Medicine, #148, Guro-dong, Guro-gu, 
      Seoul, 08308 Korea. ISNI: 0000 0001 0840 2678. GRID: grid.222754.4
FAU - Park, Kyeongsoon
AU  - Park K
AD  - 4Department of Systems Biotechnology, College of Biotechnology and Natural 
      Resources, Chung-Ang University, 4726 Seodong-daero, Daedeok-myeon, Anseong-si, 
      Gyeonggi-do 17546 Korea. ISNI: 0000 0001 0789 9563. GRID: grid.254224.7
FAU - Song, Hae-Ryong
AU  - Song HR
AD  - 2Department of Orthopedic Surgery and Rare Diseases Institute, Korea University 
      Guro Hospital, Korea University College of Medicine, #148, Guro-dong, Guro-gu, 
      Seoul, 08308 Korea. ISNI: 0000 0001 0840 2678. GRID: grid.222754.4
LA  - eng
PT  - Journal Article
DEP - 20180503
PL  - Korea (South)
TA  - Tissue Eng Regen Med
JT  - Tissue engineering and regenerative medicine
JID - 101699923
PMC - PMC6171684
OTO - NOTNLM
OT  - Adipose-derived stem cells
OT  - Chondrogenesis
OT  - Hyaluronic acid-beta-cyclodextrin
OT  - Simvastatin
COIS- The authors declare no conflict of interest.Animal experimental procedures were 
      approved by the Institutional Animal Care and Use Committee of the Korea 
      University Medical Center (KUIACUC-2014-268 and KUIACUC-2014-5).
EDAT- 2019/01/04 06:00
MHDA- 2019/01/04 06:01
PMCR- 2019/05/03
CRDT- 2019/01/04 06:00
PHST- 2017/12/11 00:00 [received]
PHST- 2018/02/26 00:00 [revised]
PHST- 2018/04/06 00:00 [accepted]
PHST- 2019/01/04 06:00 [entrez]
PHST- 2019/01/04 06:00 [pubmed]
PHST- 2019/01/04 06:01 [medline]
PHST- 2019/05/03 00:00 [pmc-release]
AID - 119 [pii]
AID - 10.1007/s13770-018-0119-9 [doi]
PST - epublish
SO  - Tissue Eng Regen Med. 2018 May 3;15(3):263-274. doi: 10.1007/s13770-018-0119-9. 
      eCollection 2018 Jun.