PMID- 30603595 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200225 IS - 2212-5469 (Electronic) IS - 1738-2696 (Print) IS - 1738-2696 (Linking) VI - 15 IP - 6 DP - 2018 Dec TI - The Expression of Immunomodulation-Related Cytokines and Genes of Adipose- and Bone Marrow-Derived Human Mesenchymal Stromal Cells from Early to Late Passages. PG - 771-779 LID - 10.1007/s13770-018-0147-5 [doi] AB - BACKGROUND: Mesenchymal stromal cells (MSCs) are multipotent stem cells that can differentiate into several cell types. In addition, many studies have shown that MSCs modulate the immune response. However, little information is currently available regarding the maintenance of immunomodulatory characteristics of MSCs through passages. Therefore, we investigated and compared cytokine and gene expression levels from adipose (AD) and bone marrow (BM)-derived MSCs relevant to immune modulation from early to late passages. METHODS: MSC immunophenotype, growth characteristics, cytokine expressions, and gene expressions were analyzed. RESULTS: AD-MSCs and BM-MSCs had similar cell morphologies and surface marker expressions from passage 4 to passage 10. Cytokines secreted by AD-MSCs and BM-MSCs were similar from early to late passages. AD-MSCs and BM-MSCs showed similar immunomodulatory properties in terms of cytokine secretion levels. However, the gene expressions of tumor necrosis factor-stimulated gene (TSG)-6 and human leukocyte antigen (HLA)-G were decreased and gene expressions of galectin-1 and -3 were increased in both AD- and BM-MSCs with repeated passages. CONCLUSION: Our study showed that the immunophenotype and expression of immunomodulation-related cytokines of AD-MSCs and BM-MSCs immunomodulation through the passages were not significantly different, even though the gene expressions of both MSCs were different. FAU - Mun, Chin Hee AU - Mun CH AD - 1Division of Rheumatology, Department of Internal Medicine, and Department of Medical Sciences, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 AD - 2BK21 Plus Project, Department of Medical Sciences, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 AD - 3Severance Biomedical Science Institute, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 FAU - Kang, Mi-Il AU - Kang MI AD - 4Division of Rheumatology, Department of Internal Medicine, Dankook University College of Medicine, 201 Manghyang-ro, Dongnam-gu, Cheonan, Chung Nam Republic of Korea. ISNI: 0000 0001 0705 4288. GRID: grid.411982.7 FAU - Shin, Yong Dae AU - Shin YD AD - 1Division of Rheumatology, Department of Internal Medicine, and Department of Medical Sciences, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 AD - 2BK21 Plus Project, Department of Medical Sciences, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 FAU - Kim, Yeseul AU - Kim Y AD - 1Division of Rheumatology, Department of Internal Medicine, and Department of Medical Sciences, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 FAU - Park, Yong-Beom AU - Park YB AD - 1Division of Rheumatology, Department of Internal Medicine, and Department of Medical Sciences, Institute for Immunology and Immunological Disease, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 AD - 2BK21 Plus Project, Department of Medical Sciences, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 AD - 3Severance Biomedical Science Institute, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722 Republic of Korea. ISNI: 0000 0004 0470 5454. GRID: grid.15444.30 LA - eng PT - Journal Article DEP - 20180828 PL - Korea (South) TA - Tissue Eng Regen Med JT - Tissue engineering and regenerative medicine JID - 101699923 EIN - Tissue Eng Regen Med. 2019 Jan 12;16(1):103-105. PMID: 30815355 PMC - PMC6250650 OTO - NOTNLM OT - Cytokine OT - Gene expression OT - Immune modulation OT - Mesenchymal stromal cells OT - Passage COIS- The authors declare that they have no conflict of interest.BM-MSCs were provided at passage 2 by the Cell Therapy Center, Severance Hospital (Yonsei University College of Medicine, Seoul, Korea) from three donors after taking their consent. AD-MSCs were obtained from two donors (32- and 41-year old females) undergoing plastic surgery, after written informed consent, in accordance with the Institutional Review Boards (4-2010-0236) of Severance Hospital and one donor from ThermoFisher Scientific (R7788115). EDAT- 2019/01/04 06:00 MHDA- 2019/01/04 06:01 PMCR- 2019/08/28 CRDT- 2019/01/04 06:00 PHST- 2018/03/08 00:00 [received] PHST- 2018/07/16 00:00 [revised] PHST- 2018/07/20 00:00 [accepted] PHST- 2019/01/04 06:00 [entrez] PHST- 2019/01/04 06:00 [pubmed] PHST- 2019/01/04 06:01 [medline] PHST- 2019/08/28 00:00 [pmc-release] AID - 147 [pii] AID - 10.1007/s13770-018-0147-5 [doi] PST - epublish SO - Tissue Eng Regen Med. 2018 Aug 28;15(6):771-779. doi: 10.1007/s13770-018-0147-5. eCollection 2018 Dec.