PMID- 30604696 OWN - NLM STAT- MEDLINE DCOM- 20200518 LR - 20200518 IS - 1745-7262 (Electronic) IS - 1008-682X (Print) IS - 1008-682X (Linking) VI - 21 IP - 4 DP - 2019 Jul-Aug TI - MicroRNA expression profile in chronic nonbacterial prostatitis revealed by next-generation small RNA sequencing. PG - 351-359 LID - 10.4103/aja.aja_97_18 [doi] AB - MicroRNAs (miRNAs) are considered to be involved in the pathogenic initiation and progression of chronic nonbacterial prostatitis (CNP); however, the comprehensive expression profile of dysregulated miRNAs, relevant signaling pathways, and core machineries in CNP have not been fully elucidated. In the current research, CNP rat models were established through the intraprostatic injection of carrageenan into the prostate. Then, next-generation sequencing was performed to explore the miRNA expression profile in CNP. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) bioinformatical analyses were conducted to reveal the enriched biological processes, molecular functions, and cellular components and signaling pathways. As a result, 1224, 1039, and 1029 known miRNAs were annotated in prostate tissues from the blank control (BC), normal saline injection (NS), and carrageenan injection (CAR) groups (n = 3 for each group), respectively. Among them, 84 miRNAs (CAR vs BC) and 70 miRNAs (CAR vs NS) with significantly different expression levels were identified. Compared with previously reported miRNAs with altered expression in various inflammatory diseases, the majority of deregulated miRNAs in CNP, such as miR-146b-5p, miR-155-5p, miR-150-5p, and miR-139-5p, showed similar expression patterns. Moreover, bioinformatics analyses have enriched mitogen-activated protein kinase (MAPK), cyclic adenosine monophosphate (cAMP), endocytosis, mammalian target of rapamycin (mTOR), and forkhead box O (FoxO) signaling pathways. These pathways were all involved in immune response, which indicates the critical regulatory role of the immune system in CNP initiation and progression. Our investigation has presented a global view of the differentially expressed miRNAs and potential regulatory networks containing their target genes, which may be helpful for identifying the novel mechanisms of miRNAs in immune regulation and effective target-specific theragnosis for CNP. FAU - Zhang, Li AU - Zhang L AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. AD - Institute of Urology, Anhui Medical University, Hefei 230022, China. AD - Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022, China. FAU - Liu, Yi AU - Liu Y AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. AD - Institute of Urology, Anhui Medical University, Hefei 230022, China. AD - Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022, China. FAU - Chen, Xian-Guo AU - Chen XG AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. AD - Institute of Urology, Anhui Medical University, Hefei 230022, China. AD - Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022, China. FAU - Zhang, Yong AU - Zhang Y AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. FAU - Chen, Jing AU - Chen J AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. FAU - Hao, Zong-Yao AU - Hao ZY AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. AD - Institute of Urology, Anhui Medical University, Hefei 230022, China. AD - Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022, China. FAU - Fan, Song AU - Fan S AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. AD - Institute of Urology, Anhui Medical University, Hefei 230022, China. AD - Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022, China. FAU - Zhang, Li-Gang AU - Zhang LG AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. FAU - Du, He-Xi AU - Du HX AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. FAU - Liang, Chao-Zhao AU - Liang CZ AD - Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China. AD - Institute of Urology, Anhui Medical University, Hefei 230022, China. AD - Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Asian J Androl JT - Asian journal of andrology JID - 100942132 RN - 0 (MicroRNAs) SB - IM MH - Animals MH - Computational Biology MH - Databases, Genetic MH - Gene Expression Profiling MH - High-Throughput Nucleotide Sequencing MH - Male MH - MicroRNAs/*genetics/metabolism MH - Prostate/*metabolism MH - Prostatitis/*genetics/metabolism MH - Rats MH - Rats, Wistar PMC - PMC6628738 OTO - NOTNLM OT - chronic nonbacterial prostatitis OT - immune OT - inflammation OT - miRNA OT - next-generation sequencing EDAT- 2019/01/04 06:00 MHDA- 2020/05/19 06:00 PMCR- 2019/07/01 CRDT- 2019/01/04 06:00 PHST- 2019/01/04 06:00 [pubmed] PHST- 2020/05/19 06:00 [medline] PHST- 2019/01/04 06:00 [entrez] PHST- 2019/07/01 00:00 [pmc-release] AID - 248810 [pii] AID - AJA-21-351 [pii] AID - 10.4103/aja.aja_97_18 [doi] PST - ppublish SO - Asian J Androl. 2019 Jul-Aug;21(4):351-359. doi: 10.4103/aja.aja_97_18.