PMID- 30604794
OWN - NLM
STAT- MEDLINE
DCOM- 20190614
LR  - 20190614
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 7
IP  - 3
DP  - 2019 Feb 26
TI  - Heparin-reduced graphene oxide nanocomposites for curcumin delivery: in vitro, in 
      vivo and molecular dynamics simulation study.
PG  - 1011-1027
LID - 10.1039/c8bm00907d [doi]
AB  - Graphene-based nanomaterials (GBNMs) have great potential in drug delivery and 
      photothermal therapy owing to their unique physicochemical properties. However, 
      inferior water solubility and biocompatibility related issues greatly restricted 
      their further applications. Herein, to rectify the obstructive problems, we 
      prepared uniform and smaller sized graphene oxide (GO) nanosheets ( approximately 85 nm) via a 
      modified Hummers' method, which exhibited significantly improved 
      hemocompatibility compared to random large sized GO nanosheets prepared by a 
      common method. Then, we grafted unfractionated heparin (UFH) onto reduced 
      graphene oxide (rGO) covalently using adipic acid dihydrazide (ADH) as a linker 
      to fabricate biocompatible nanocomposites for the cellular delivery of curcumin 
      (Cur). The novel nanocomposites showed quite a small size of 42 nm in average 
      lateral dimension and exhibited a significantly stronger photothermal effect than 
      GO nanosheets. Besides, in vitro cell experiments verified that the potential 
      anticancer efficacy of Cur-loaded vehicles and cytotoxicity of rGO-UFH/Cur 
      against MCF-7 and A549 cells could be further enhanced under 808 nm irradiation, 
      suggesting the possibility of combinational chemotherapy and photothermal 
      therapy. Moreover, consistent with the in vitro sustained drug release 
      performance, an in vivo pharmacokinetics study also indicated that the retention 
      time of Cur could be significantly prolonged when loaded on rGO-UFH 
      nanocomposites than in free Cur solution. Notably, we firstly discussed the 
      interaction between rGO and Cur, and demonstrated the meliorative 
      biocompatibility of uniform rGO compared to GRO via a molecular dynamics 
      simulation (MD) study. Thus, the in vitro, in vivo and computational study 
      demonstrated that the small sized rGO-UFH nanocomposites had wide application 
      prospects as drug delivery vehicles.
FAU - Shi, Xiaoqun
AU  - Shi X
AD  - Department of Pharmaceutics, College of Pharmacy, Shandong University, Jinan 
      250012, China. professorgxzhai@126.com.
FAU - Wang, Yang
AU  - Wang Y
FAU - Sun, Haiyan
AU  - Sun H
FAU - Chen, Yujuan
AU  - Chen Y
FAU - Zhang, Xingzhen
AU  - Zhang X
FAU - Xu, Jiangkang
AU  - Xu J
FAU - Zhai, Guangxi
AU  - Zhai G
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 0 (Biocompatible Materials)
RN  - 0 (Proteins)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
RN  - 9005-49-6 (Heparin)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - A549 Cells
MH  - Animals
MH  - Apoptosis/drug effects/radiation effects
MH  - Biocompatible Materials/chemistry/pharmacology
MH  - Curcumin/*chemistry/metabolism/pharmacology
MH  - Drug Liberation
MH  - Graphite/*chemistry
MH  - Half-Life
MH  - Hemolysis/drug effects
MH  - Heparin/*chemistry
MH  - Humans
MH  - Infrared Rays
MH  - MCF-7 Cells
MH  - Male
MH  - Molecular Dynamics Simulation
MH  - Nanocomposites/*chemistry
MH  - Proteins/chemistry/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Distribution
EDAT- 2019/01/04 06:00
MHDA- 2019/06/15 06:00
CRDT- 2019/01/04 06:00
PHST- 2019/01/04 06:00 [pubmed]
PHST- 2019/06/15 06:00 [medline]
PHST- 2019/01/04 06:00 [entrez]
AID - 10.1039/c8bm00907d [doi]
PST - ppublish
SO  - Biomater Sci. 2019 Feb 26;7(3):1011-1027. doi: 10.1039/c8bm00907d.