PMID- 30608062
OWN - NLM
STAT- MEDLINE
DCOM- 20190304
LR  - 20200225
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 25
DP  - 2019 Jan 4
TI  - Expression of Tripartite Motif-Containing Proteactiin 11 (TRIM11) is Associated 
      with the Progression of Human Prostate Cancer and is Downregulated by 
      MicroRNA-5193.
PG  - 98-106
LID - 10.12659/MSM.911818 [doi]
AB  - BACKGROUND Tripartite motif-containing protein 11 (TRIM11), encoded by the TRIM11 
      gene, has been studied in some human malignant tumors. MicroRNA-5193 (miRNA-5193) 
      was predicted to target TRIM11, according to bioinformatics data from TargetScan. 
      However, the roles of TRIM11 and miRNA-5193 in prostate cancer remain unknown. 
      This study aimed to investigate the regulatory effects of miRNA-5193 on the 
      expression of TRIM11 in prostate cancer tissues compared with adjacent normal 
      prostate, and in human prostate cancer cell lines, PC3 and DU145 in vitro. 
      MATERIAL AND METHODS Prostate tumor tissue and adjacent normal tissue from 137 
      patients with stage T1c (n=66), stage T2 (n=48), and stage T3 (n=23) prostate 
      cancer were studied. Expression levels of the TRIM 11 protein and the TRIM11 gene 
      in prostate cancer, normal prostate tissue, and human prostate cancer cell lines, 
      PC3 and DU145, were measured by Western blot and quantitative real-time 
      polymerase chain reaction (qRT-PCR), respectively. Transfection with TRIM11 small 
      interfering RNA (siRNA) resulted in gene knockdown. Transfection with a miR-5193 
      mimic resulted in overexpression of miR-5193. Proliferation and invasion assays 
      were performed for PC3 and DU145 cells in vitro. RESULTS TRIM11 expression was 
      upregulated in prostate cancer specimens compared with normal prostate tissue and 
      was significantly correlated with reduced outcome. In human prostate cancer cell 
      lines, PC3 and DU145, TRIM11 overexpression promoted cell proliferation. 
      Upregulation of miR-5193 downregulated the expression of TRIM11. CONCLUSIONS 
      TRIM11 was upregulated in prostate cancer tissue and was associated with reduced 
      prognosis. TRIM11 expression increased cell proliferation in vitro and was 
      downregulated by miR-5193.
FAU - Pan, Yue
AU  - Pan Y
AD  - Department of Urology, Shandong Provincial Hospital Affiliated to Shandong 
      University, Jinan, Shandong, China (mainland).
AD  - Department of Urology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang, China (mainland).
FAU - Zhang, Riyan
AU  - Zhang R
AD  - School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, 
      Zhejiang, China (mainland).
FAU - Chen, Hongde
AU  - Chen H
AD  - Department of Urology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang, China (mainland).
FAU - Chen, Wei
AU  - Chen W
AD  - Department of Urology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang, China (mainland).
FAU - Wu, Keming
AU  - Wu K
AD  - Department of Urology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang, China (mainland).
FAU - Lv, Jiaju
AU  - Lv J
AD  - Department of Urology, Shandong Provincial Hospital Affiliated to Shandong 
      University, Jinan, Shandong, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20190104
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (MIRN5193 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tripartite Motif Proteins)
RN  - EC 2.3.2.27 (TRIM11 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Disease Progression
MH  - Epithelial-Mesenchymal Transition/genetics
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics/metabolism
MH  - Middle Aged
MH  - Prognosis
MH  - Prostatic Neoplasms/*genetics/metabolism/pathology
MH  - RNA, Small Interfering/metabolism
MH  - Tripartite Motif Proteins/*genetics/metabolism
MH  - Ubiquitin-Protein Ligases/*genetics/metabolism
PMC - PMC6329255
EDAT- 2019/01/05 06:00
MHDA- 2019/03/05 06:00
PMCR- 2019/01/04
CRDT- 2019/01/05 06:00
PHST- 2019/01/05 06:00 [entrez]
PHST- 2019/01/05 06:00 [pubmed]
PHST- 2019/03/05 06:00 [medline]
PHST- 2019/01/04 00:00 [pmc-release]
AID - 911818 [pii]
AID - 10.12659/MSM.911818 [doi]
PST - epublish
SO  - Med Sci Monit. 2019 Jan 4;25:98-106. doi: 10.12659/MSM.911818.