PMID- 30608700
OWN - NLM
STAT- MEDLINE
DCOM- 20200528
LR  - 20210218
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Print)
IS  - 1535-3893 (Linking)
VI  - 18
IP  - 3
DP  - 2019 Mar 1
TI  - Quantitative Proteomic Analysis of Small and Large Extracellular Vesicles (EVs) 
      Reveals Enrichment of Adhesion Proteins in Small EVs.
PG  - 947-959
LID - 10.1021/acs.jproteome.8b00647 [doi]
AB  - Extracellular vesicles (EVs) are important mediators of cell-cell communication 
      due to their cargo content of proteins, lipids, and RNAs. We previously reported 
      that small EVs (SEVs) called exosomes promote directed and random cell motility, 
      invasion, and serum-independent growth. In contrast, larger EVs (LEVs) were not 
      active in those assays, but might have unique functional properties. In order to 
      identify protein cargos that may contribute to different functions of SEVs and 
      LEVs, we used isobaric tags for relative and absolute quantitation (iTRAQ)-liquid 
      chromatography (LC) tandem mass spectrometry (MS) on EVs isolated from a colon 
      cancer cell line. Bioinformatics analyses revealed that SEVs are enriched in 
      proteins associated with cell-cell junctions, cell-matrix adhesion, exosome 
      biogenesis machinery, and various signaling pathways. In contrast, LEVs are 
      enriched in proteins associated with ribosome and RNA biogenesis, processing, and 
      metabolism. Western blot analysis of EVs purified from two different cancer cell 
      types confirmed the enrichment of cell-matrix and cell-cell adhesion proteins in 
      SEVs. Consistent with those data, we found that cells exhibit enhanced adhesion 
      to surfaces coated with SEVs compared to an equal protein concentration of LEVs. 
      These data suggest that a major function of SEVs is to promote cellular adhesion.
FAU - Jimenez, Lizandra
AU  - Jimenez L
AUID- ORCID: 0000-0002-5024-4383
AD  - Department of Cell and Developmental Biology , Vanderbilt University School of 
      Medicine , Nashville , Tennessee 37232 , United States.
FAU - Yu, Hui
AU  - Yu H
AUID- ORCID: 0000-0002-4491-8855
AD  - Department of Internal Medicine , University of New Mexico , Albuquerque , New 
      Mexico 87131 , United States.
FAU - McKenzie, Andrew J
AU  - McKenzie AJ
AUID- ORCID: 0000-0002-5088-1876
AD  - Sarah Cannon Research Institute , Nashville , Tennessee 37203 , United States.
FAU - Franklin, Jeffrey L
AU  - Franklin JL
AUID- ORCID: 0000-0002-7111-4449
AD  - Department of Cell and Developmental Biology , Vanderbilt University School of 
      Medicine , Nashville , Tennessee 37232 , United States.
AD  - Department of Medicine , Vanderbilt University Medical Center , Nashville , 
      Tennessee 37212 , United States.
FAU - Patton, James G
AU  - Patton JG
AD  - Department of Biological Sciences , Vanderbilt University School of Medicine , 
      Nashville , Tennessee 37212 , United States.
FAU - Liu, Qi
AU  - Liu Q
AD  - Department of Biostatistics , Vanderbilt University Medical Center , Nashville , 
      Tennessee 37232 , United States.
FAU - Weaver, Alissa M
AU  - Weaver AM
AUID- ORCID: 0000-0002-4096-8636
AD  - Department of Cell and Developmental Biology , Vanderbilt University School of 
      Medicine , Nashville , Tennessee 37232 , United States.
AD  - Department of Pathology, Microbiology and Immunology , Vanderbilt University 
      Medical Center , Nashville , Tennessee 37212 , United States.
LA  - eng
GR  - R01 GM117916/GM/NIGMS NIH HHS/United States
GR  - T32 CA119925/CA/NCI NIH HHS/United States
GR  - P30 DK058404/DK/NIDDK NIH HHS/United States
GR  - F32 CA217064/CA/NCI NIH HHS/United States
GR  - U19 CA179514/CA/NCI NIH HHS/United States
GR  - UL1 TR002243/TR/NCATS NIH HHS/United States
GR  - U54 CA217450/CA/NCI NIH HHS/United States
GR  - R01 CA206458/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20190123
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Cell Adhesion Molecules)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules/*analysis
MH  - Cell Line, Tumor
MH  - Chromatography, Liquid
MH  - Exosomes/chemistry/physiology
MH  - Extracellular Vesicles/*chemistry/physiology
MH  - Humans
MH  - Particle Size
MH  - Proteomics/*methods
MH  - Tandem Mass Spectrometry
PMC - PMC6411421
MID - NIHMS1009179
OTO - NOTNLM
OT  - adhesion
OT  - exosomes
OT  - extracellular vesicles
OT  - iTRAQ
OT  - microvesicles
OT  - proteomics
COIS- Conflict of interest statement No potential conflicts of interest were disclosed.
EDAT- 2019/01/05 06:00
MHDA- 2020/05/29 06:00
PMCR- 2020/03/01
CRDT- 2019/01/05 06:00
PHST- 2019/01/05 06:00 [pubmed]
PHST- 2020/05/29 06:00 [medline]
PHST- 2019/01/05 06:00 [entrez]
PHST- 2020/03/01 00:00 [pmc-release]
AID - 10.1021/acs.jproteome.8b00647 [doi]
PST - ppublish
SO  - J Proteome Res. 2019 Mar 1;18(3):947-959. doi: 10.1021/acs.jproteome.8b00647. 
      Epub 2019 Jan 23.