PMID- 30609499
OWN - NLM
STAT- MEDLINE
DCOM- 20190221
LR  - 20210816
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 218
DP  - 2019 Mar
TI  - The endocrine-disrupting potential of four chlorophenols by in vitro and in 
      silico assay.
PG  - 941-947
LID - S0045-6535(18)32306-3 [pii]
LID - 10.1016/j.chemosphere.2018.11.199 [doi]
AB  - Chlorophenols (CPs) have mainly been used as a biocide, wood treatment agent and 
      a byproduct of bleaching in paper mills. They have been a topic of concern due to 
      their wide spread and potential effects on human and wildlife. However, data on 
      the thresholds and effects of the number of chlorine atoms on the 
      endocrine-disrupting potential of CPs remain scarce. In this study, we adopted 
      two in vitro models (reporter gene assays and H295R cell line) to investigate the 
      endocrine-disrupting effects of four CPs (pentachlorophenol (PCP), 
      2,4,6-trichlorophenol (2,4,6-TCP), 2,4-dichlorophenol (2,4-DCP) and 
      2-chlorophenol (2-CP)). The molecular docking platform was adopted to further 
      confirm the results of the in vitro assessment. Our results revealed that PCP 
      exhibited oestrogen receptor alpha (ERalpha) agonistic activity at the concentration 
      of 10(-5) M and the value of REC(20) was 1.9 x 10(-6) M. PCP and 2, 4, 6-TCP 
      showed anti-oestrogenic activities with a RIC(20) value of 2.8 x 10(-7)and 
      2.9 x 10(-6) M, respectively. Notably, only PCP exhibited thyroid hormone 
      receptor beta (TRbeta) antagonistic activity occurred at the concentration of 
      10(-5) M, with a RIC(20) value of 1.3 x 10(-6) M. The oestrogenic and thyroid 
      hormone effects of CPs may be dependent on the number of chlorine atoms. A higher 
      number of chlorine atoms indicated the higher effect of four CPs. The results of 
      molecular docking were consistent with the reporter gene assay. For H295R cell 
      line assay, PCP induced the StAR upregulation, while CYP17 was downregulated in a 
      concentration-dependent manner by PCP and 2, 4, 6-TCP.
CI  - Copyright (c) 2018 Elsevier Ltd. All rights reserved.
FAU - Yu, Chang
AU  - Yu C
AD  - Key Laboratory of Microbial Technology for Industrial Pollution Control of 
      Zhejiang Province, College of Environment, Zhejiang University of Technology, 
      Hangzhou, Zhejiang, 310032, China.
FAU - Wang, Chi
AU  - Wang C
AD  - Longyou Huashui Drinking Water Industry Company, Quzhou, Zhejiang, 324400, China.
FAU - Lu, Zhengbiao
AU  - Lu Z
AD  - Key Laboratory of Microbial Technology for Industrial Pollution Control of 
      Zhejiang Province, College of Environment, Zhejiang University of Technology, 
      Hangzhou, Zhejiang, 310032, China.
FAU - Zhang, Chen
AU  - Zhang C
AD  - School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, Guangdong, 
      510006, China.
FAU - Dai, Wei
AU  - Dai W
AD  - Key Laboratory of Microbial Technology for Industrial Pollution Control of 
      Zhejiang Province, College of Environment, Zhejiang University of Technology, 
      Hangzhou, Zhejiang, 310032, China.
FAU - Yu, Shuqing
AU  - Yu S
AD  - Key Laboratory of Microbial Technology for Industrial Pollution Control of 
      Zhejiang Province, College of Environment, Zhejiang University of Technology, 
      Hangzhou, Zhejiang, 310032, China.
FAU - Lin, Shu
AU  - Lin S
AD  - Key Laboratory of Microbial Technology for Industrial Pollution Control of 
      Zhejiang Province, College of Environment, Zhejiang University of Technology, 
      Hangzhou, Zhejiang, 310032, China.
FAU - Zhang, Quan
AU  - Zhang Q
AD  - Key Laboratory of Microbial Technology for Industrial Pollution Control of 
      Zhejiang Province, College of Environment, Zhejiang University of Technology, 
      Hangzhou, Zhejiang, 310032, China. Electronic address: quanzhang@zjut.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20181129
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Chlorophenols)
RN  - 0 (ESR1 protein, human)
RN  - 0 (Endocrine Disruptors)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Thyroid Hormone Receptors beta)
SB  - IM
MH  - Cell Line
MH  - Chlorophenols/*pharmacology
MH  - Endocrine Disruptors/*pharmacology
MH  - Endocrine System
MH  - Estrogen Receptor alpha/agonists
MH  - Humans
MH  - Molecular Docking Simulation
MH  - Thyroid Hormone Receptors beta/antagonists & inhibitors
OTO - NOTNLM
OT  - Chlorophenols
OT  - Endocrine-disrupting effects
OT  - H295R
OT  - Molecular docking
EDAT- 2019/01/06 06:00
MHDA- 2019/02/23 06:00
CRDT- 2019/01/06 06:00
PHST- 2018/10/08 00:00 [received]
PHST- 2018/11/27 00:00 [revised]
PHST- 2018/11/28 00:00 [accepted]
PHST- 2019/01/06 06:00 [entrez]
PHST- 2019/01/06 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
AID - S0045-6535(18)32306-3 [pii]
AID - 10.1016/j.chemosphere.2018.11.199 [doi]
PST - ppublish
SO  - Chemosphere. 2019 Mar;218:941-947. doi: 10.1016/j.chemosphere.2018.11.199. Epub 
      2018 Nov 29.