PMID- 30609512
OWN - NLM
STAT- MEDLINE
DCOM- 20190304
LR  - 20190304
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 194
DP  - 2019 Mar 1
TI  - In situ formation of gold nanoparticles in polymer inclusion membrane: 
      Application as platform in a label-free potentiometric immunosensor for 
      Salmonella typhimurium detection.
PG  - 134-142
LID - S0039-9140(18)31060-9 [pii]
LID - 10.1016/j.talanta.2018.10.024 [doi]
AB  - Polymeric ion selective electrodes are highly sensitive to changes in zero 
      current ion flow and this offers a route to signal amplification in label-free 
      potentiometric immunosensors. In this work, a label-free potentiometric 
      immunosensor toward Salmonella typhimurium (ST) assembled in a home-made 
      pipette-tip electrode is described. The signal-output amplification was 
      implemented on a gold nanoparticle polymer inclusion membrane (AuNPs-PIM) which 
      was used as sensing platform and for antibody immobilization. Additionally, a 
      marker ion was used to detect the antibody-antigen binding event at the electrode 
      surface. The immunosensor construction was performed in several steps: i) gold 
      salt ions extraction in PVC membrane; ii) AuNPs formation using Na(2)EDTA as 
      reduction agent; iii) antibody anti-Salmonella conjugation on AuNPs-PIM in 
      pipette-tip electrodes. The potential shift observed in potentiometric 
      measurements was derived simply from the blocking effect in the ionic flux caused 
      by antigen-antibody conjugation, without no extra steps, mimetizing the 
      ion-channel sensors. A detection limit of 6 cells mL(-1) was attained. As 
      proof-of-concept, recovery studies were performed in spiked commercial apple 
      juice samples with success. Due to the simplicity of use, the appealing cost of 
      equipment and sensor production and being able to provide a quick analytical 
      response (less than 1 h for a complete assay, including sample preparation for 
      analysis), this scheme represents a good prototype device for the detection of 
      foodborne pathogens like ST or other immune-responsive bacteria.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Silva, Nadia F D
AU  - Silva NFD
AD  - REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politecnico 
      do Porto, 4200-072 Porto, Portugal; REQUIMTE/LAQV, Departamento de Quimica e 
      Bioquimica, Faculdade de Ciencias, Universidade do Porto, 4169-007 Porto, 
      Portugal.
FAU - Magalhaes, Julia M C S
AU  - Magalhaes JMCS
AD  - REQUIMTE/LAQV, Departamento de Engenharia Quimica, Faculdade de Engenharia, 
      Universidade do Porto, 4200-465 Porto, Portugal. Electronic address: 
      jmagalhaes@reit.up.pt.
FAU - Barroso, M Fatima
AU  - Barroso MF
AD  - REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politecnico 
      do Porto, 4200-072 Porto, Portugal.
FAU - Oliva-Teles, Teresa
AU  - Oliva-Teles T
AD  - REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politecnico 
      do Porto, 4200-072 Porto, Portugal.
FAU - Freire, Cristina
AU  - Freire C
AD  - REQUIMTE/LAQV, Departamento de Quimica e Bioquimica, Faculdade de Ciencias, 
      Universidade do Porto, 4169-007 Porto, Portugal.
FAU - Delerue-Matos, Cristina
AU  - Delerue-Matos C
AD  - REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politecnico 
      do Porto, 4200-072 Porto, Portugal.
LA  - eng
PT  - Journal Article
DEP - 20181012
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Membranes, Artificial)
RN  - 0 (Polymers)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Electrochemistry
MH  - Fruit and Vegetable Juices/microbiology
MH  - Gold/*chemistry
MH  - Immunoassay/*methods
MH  - Malus/chemistry
MH  - *Membranes, Artificial
MH  - Metal Nanoparticles/*chemistry
MH  - Polymers/*chemistry
MH  - Salmonella typhimurium/*isolation & purification
OTO - NOTNLM
OT  - Gold nanoparticles
OT  - Immunosensor
OT  - Polymer inclusion membranes
OT  - Potentiometry
OT  - Salmonella typhimurium
EDAT- 2019/01/06 06:00
MHDA- 2019/03/05 06:00
CRDT- 2019/01/06 06:00
PHST- 2018/07/05 00:00 [received]
PHST- 2018/10/04 00:00 [revised]
PHST- 2018/10/08 00:00 [accepted]
PHST- 2019/01/06 06:00 [entrez]
PHST- 2019/01/06 06:00 [pubmed]
PHST- 2019/03/05 06:00 [medline]
AID - S0039-9140(18)31060-9 [pii]
AID - 10.1016/j.talanta.2018.10.024 [doi]
PST - ppublish
SO  - Talanta. 2019 Mar 1;194:134-142. doi: 10.1016/j.talanta.2018.10.024. Epub 2018 
      Oct 12.