PMID- 30611612
OWN - NLM
STAT- MEDLINE
DCOM- 20190705
LR  - 20190705
IS  - 1097-4180 (Electronic)
IS  - 1074-7613 (Linking)
VI  - 50
IP  - 1
DP  - 2019 Jan 15
TI  - Transcription Factor PU.1 Promotes Conventional Dendritic Cell Identity and 
      Function via Induction of Transcriptional Regulator DC-SCRIPT.
PG  - 77-90.e5
LID - S1074-7613(18)30492-8 [pii]
LID - 10.1016/j.immuni.2018.11.010 [doi]
AB  - Dendritic cells (DCs) are can be broadly divided into conventional (cDC) and 
      plasmacytoid (pDC) subsets. Despite the importance of this lineage diversity, its 
      genetic basis is not fully understood. We found that conditional ablation of the 
      Ets-family transcription factor PU.1 in DC-restricted progenitors led to 
      increased pDC production at the expense of cDCs. PU.1 controlled many of the 
      cardinal functions of DCs, such as antigen presentation by cDCs and type I 
      interferon production by pDCs. Conditional ablation of PU.1 de-repressed the pDC 
      transcriptional signature in cDCs. The combination of genome-wide mapping of PU.1 
      binding and gene expression analysis revealed a key role for PU.1 in maintaining 
      cDC identity through the induction of the transcriptional regulator DC-SCRIPT. 
      PU.1 activated DC-SCRIPT expression, which in turn promoted cDC formation, 
      particularly of cDC1s, and repressed pDC development. Thus, cDC identity is 
      regulated by a transcriptional node requiring PU.1 and DC-SCRIPT.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Chopin, Michael
AU  - Chopin M
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia. Electronic address: chopin@wehi.edu.au.
FAU - Lun, Aaron T
AU  - Lun AT
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Zhan, Yifan
AU  - Zhan Y
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Schreuder, Jaring
AU  - Schreuder J
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Coughlan, Hannah
AU  - Coughlan H
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - D'Amico, Angela
AU  - D'Amico A
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Mielke, Lisa A
AU  - Mielke LA
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia; Olivia Newton-John Cancer Research Institute and La Trobe 
      University School of Cancer Medicine, Heidelberg, VIC 3084, Australia.
FAU - Almeida, Francisca F
AU  - Almeida FF
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Kueh, Andrew J
AU  - Kueh AJ
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Dickins, Ross A
AU  - Dickins RA
AD  - Australian Centre for Blood Diseases, Monash University, Melbourne, VIC 3004, 
      Australia.
FAU - Belz, Gabrielle T
AU  - Belz GT
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Naik, Shalin H
AU  - Naik SH
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Lew, Andrew M
AU  - Lew AM
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia; Department of Microbiology and Immunology, University of 
      Melbourne, Parkville, VIC 3010, Australia.
FAU - Bouillet, Phillipe
AU  - Bouillet P
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Herold, Marco J
AU  - Herold MJ
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Smyth, Gordon K
AU  - Smyth GK
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; School of Mathematics and Statistics, University of Melbourne, 
      Parkville, VIC 3010, Australia.
FAU - Corcoran, Lynn M
AU  - Corcoran LM
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia.
FAU - Nutt, Stephen L
AU  - Nutt SL
AD  - Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3052, 
      Australia; Department of Medical Biology, University of Melbourne, Parkville, VIC 
      3010, Australia. Electronic address: nutt@wehi.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190102
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (DC-SCRIPT protein, mouse)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interferon Type I)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (proto-oncogene protein Spi-1)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Dendritic Cells/*physiology
MH  - Gene Expression Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - Interferon Type I/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Signal Transduction
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription Factors/genetics/*metabolism
MH  - Transcriptome
OTO - NOTNLM
OT  - DC-SCRIPT
OT  - PU.1
OT  - cell differentiation
OT  - dendritic cell
OT  - plasmacytoid dendritic cell
OT  - transcription factor
EDAT- 2019/01/07 06:00
MHDA- 2019/07/06 06:00
CRDT- 2019/01/07 06:00
PHST- 2017/07/24 00:00 [received]
PHST- 2018/09/20 00:00 [revised]
PHST- 2018/11/02 00:00 [accepted]
PHST- 2019/01/07 06:00 [pubmed]
PHST- 2019/07/06 06:00 [medline]
PHST- 2019/01/07 06:00 [entrez]
AID - S1074-7613(18)30492-8 [pii]
AID - 10.1016/j.immuni.2018.11.010 [doi]
PST - ppublish
SO  - Immunity. 2019 Jan 15;50(1):77-90.e5. doi: 10.1016/j.immuni.2018.11.010. Epub 
      2019 Jan 2.