PMID- 30612176
OWN - NLM
STAT- MEDLINE
DCOM- 20190221
LR  - 20190221
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 411
IP  - 5
DP  - 2019 Feb
TI  - A novel magnetic fluorescent biosensor based on graphene quantum dots for rapid, 
      efficient, and sensitive separation and detection of circulating tumor cells.
PG  - 985-995
LID - 10.1007/s00216-018-1501-0 [doi]
AB  - We describe a "turn-on" magnetic fluorescent biosensor based on graphene quantum 
      dots (GQDs), Fe(3)O(4), and molybdenum disulfide (MoS(2)) nanosheets. It is used 
      for rapid, efficient, and sensitive separation and detection of circulating tumor 
      cells (CTCs). A facile approach (electrochemical synthesis method) for the 
      preparation of photoluminescent GQDs functionalized with an aptamer [epithelial 
      cell adhesion molecule (EpCAM) receptors] and a magnetic agent for one-step 
      bioimaging and enrichment of CTCs is described. MoS(2) nanosheets, as a 
      fluorescence quencher, and the aforementioned aptamer@Fe(3)O(4)@GQD complex were 
      assembled to construct "turn-on" biosensing magnetic fluorescent nanocomposites 
      (MFNs). This system exhibits low cytotoxicity and an average capture efficiency 
      of 90%, which is higher than that of other magnetic nanoparticles on account of 
      the one-step CTC separation method. In addition, the MFNs could quickly identify 
      and label CTCs within 15 min, surpassing other one-step and two-step marker 
      detection methods. Furthermore, because of the presence of aptamers, the MFNs 
      have specific capability to capture CTCs (both low- and high-EpCAM-expressing 
      cells). In addition, high-sensitivity detection of up to ten tumor cells in whole 
      blood was achieved. Therefore, the MFNs have great potential to be used as 
      universal biosensing nanocomposites for fluorescence-guided tumor cell enrichment 
      and bioimaging. Graphical abstract ᅟ.
FAU - Cui, Fangchao
AU  - Cui F
AD  - School of Food Science, State Key Laboratory of Food Science and Technology, 
      National Engineering Research Center for Functional Foods, School of Food Science 
      Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 
      Wuxi, 214122, Jiangsu, China.
FAU - Ji, Jian
AU  - Ji J
AD  - School of Food Science, State Key Laboratory of Food Science and Technology, 
      National Engineering Research Center for Functional Foods, School of Food Science 
      Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 
      Wuxi, 214122, Jiangsu, China.
FAU - Sun, Jiadi
AU  - Sun J
AD  - School of Food Science, State Key Laboratory of Food Science and Technology, 
      National Engineering Research Center for Functional Foods, School of Food Science 
      Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 
      Wuxi, 214122, Jiangsu, China.
FAU - Wang, Jun
AU  - Wang J
AD  - Department of Toxicology, Key Laboratory of Modern Toxicology (NJMU), Ministry of 
      Education, School of Public Health, Nanjing Medical University, Nanjing, 211166, 
      Jiangsu, China.
FAU - Wang, Haiming
AU  - Wang H
AD  - Guangzhou GRG Metrology & Test Co. Ltd, Guangzhou, 510000, Guangdong, China.
FAU - Zhang, Yinzhi
AU  - Zhang Y
AD  - School of Food Science, State Key Laboratory of Food Science and Technology, 
      National Engineering Research Center for Functional Foods, School of Food Science 
      Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 
      Wuxi, 214122, Jiangsu, China.
FAU - Ding, Hong
AU  - Ding H
AD  - National Institutes for Food and Drug Control, Beijing, 100050, China.
FAU - Lu, Yong
AU  - Lu Y
AD  - National Institutes for Food and Drug Control, Beijing, 100050, China.
FAU - Xu, Dan
AU  - Xu D
AD  - School of Food and Biological Engineering, Shaanxi University of Science and 
      Technology, Xi'an, 710021, Shaanxi, China.
FAU - Sun, Xiulan
AU  - Sun X
AD  - School of Food Science, State Key Laboratory of Food Science and Technology, 
      National Engineering Research Center for Functional Foods, School of Food Science 
      Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, 
      Wuxi, 214122, Jiangsu, China. sxlzyz@jiangnan.edu.cn.
LA  - eng
GR  - No. 201513006, No. 31371768, 2016YFD0401204/the National Research Program of 
      China/
PT  - Journal Article
DEP - 20190105
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Disulfides)
RN  - 0 (Epithelial Cell Adhesion Molecule)
RN  - 0 (Fluorescent Dyes)
RN  - 7782-42-5 (Graphite)
RN  - 81AH48963U (Molybdenum)
RN  - XM0M87F357 (Ferrosoferric Oxide)
RN  - ZC8B4P503V (molybdenum disulfide)
SB  - IM
MH  - A549 Cells
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Cell Separation/*instrumentation/methods
MH  - Disulfides/chemistry
MH  - Epithelial Cell Adhesion Molecule/analysis
MH  - Equipment Design
MH  - Ferrosoferric Oxide/chemistry
MH  - Fluorescent Dyes/*chemistry
MH  - Graphite/*chemistry
MH  - HEK293 Cells
MH  - Hep G2 Cells
MH  - Humans
MH  - Molybdenum/chemistry
MH  - Nanostructures/chemistry/ultrastructure
MH  - Neoplasms/blood/pathology
MH  - Neoplastic Cells, Circulating/*pathology
MH  - Quantum Dots/*chemistry
OTO - NOTNLM
OT  - Aptamer
OT  - Circulating tumor cells
OT  - Graphene quantum dots
OT  - Magnetic fluorescence biosensor
OT  - Magnetic separation
EDAT- 2019/01/07 06:00
MHDA- 2019/02/23 06:00
CRDT- 2019/01/07 06:00
PHST- 2018/08/15 00:00 [received]
PHST- 2018/11/16 00:00 [accepted]
PHST- 2018/11/08 00:00 [revised]
PHST- 2019/01/07 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
PHST- 2019/01/07 06:00 [entrez]
AID - 10.1007/s00216-018-1501-0 [pii]
AID - 10.1007/s00216-018-1501-0 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2019 Feb;411(5):985-995. doi: 10.1007/s00216-018-1501-0. Epub 
      2019 Jan 5.