PMID- 30613011
OWN - NLM
STAT- MEDLINE
DCOM- 20190221
LR  - 20200226
IS  - 1673-4254 (Print)
IS  - 2663-0842 (Electronic)
IS  - 1673-4254 (Linking)
VI  - 38
IP  - 12
DP  - 2018 Dec 30
TI  - [Cancer stem-like cell-derived exosomes promotes the proliferation and invasion 
      of human umbilical cord blood-derived mesenchymal stem cells].
PG  - 1440-1447
LID - 10.12122/j.issn.1673-4254.2018.12.07 [doi]
AB  - OBJECTIVE: To investigate the effect of Piwil2-induced cancer stem-like cell 
      (Piwil2-iCSC)-derived exosomes on the proliferation,migration and invasion of 
      human umbilical cord blood-derived mesenchymal stem cells (hucMSCs). METHODS: 
      Piwil2-iCSC-derived exosomes were isolated by ultracentrifugation and identified 
      using transmission electron microscopy,nanoparticle tracking analysis and Western 
      blotting.Exosome uptake assay was used to identify the pathway that 
      Piwil2-iCSCderived exosomes utilized.HucMSCs were divided into control group,PBS 
      intervention group and exosome intervention group,and CCK-8 assay,wound healing 
      assay,Transwell assay,Western blotting and cell karyotype analysis were used to 
      observe the proliferation,migration,invasion,expression levels of MMP2 and MMP9 
      proteins,and chromosome structure of hucMSCs. RESULTS: The diameter of 
      Piwil2-iCSC-derived exosomes ranged from 50 nm to 100 nm,and most of them were 
      oval or spherical capsules rich in CD9,CD63 and Piwil2 proteins.Exosomal uptake 
      assay showed that the exosomes executed theirs functions after entering the 
      cells.Compared with the control cells and PBS-treated cells,hucMSCs treated with 
      the exosomes showed significantly increased number of proliferating cells 
      (P<0.05) with accelerated healing rate (P<0.05 at 24 h;P<0.01 at 48 
      h),increased invasive cells (P<0.01),enhanced protein expressions of 
      MMP2(P<0.05 vs PBS group;P<0.01 vs control group) and MMP9(P<0.05),but 
      their karyotype still remained 46XY without any abnormalities. CONCLUSIONS: 
      Piwil2-iCSC-derived exosomes can promote the proliferation,migration and invasion 
      but does not cause cancer-like heterogeneity changes in hucMSCs.
FAU - Zhang, Dan
AU  - Zhang D
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
FAU - He, Dawei
AU  - He D
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
FAU - Li, Dian
AU  - Li D
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
FAU - Tang, Bo
AU  - Tang B
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
FAU - Hu, Dong
AU  - Hu D
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
FAU - Guo, Wenhao
AU  - Guo W
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
FAU - Wang, Zhang
AU  - Wang Z
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
FAU - Shen, Lianju
AU  - Shen L
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
FAU - Wei, Guanghui
AU  - Wei G
AD  - ZHANG Dan, HE Dawei, LI Dian, TANG Bo, HU Dong, GUO Wenhao, WANG Zhang, SHEN 
      Lianju, WEI Guanghui Department of Urology, Children's Hospital of Chongqing 
      Medical University, Chongqing Key Laboratory of Children Urogenital Development 
      and Tissue Engineering, Ministry of Education Key Laboratory of Child Development 
      and Disorders, China International Science and Technology Cooperation base of 
      Child development and Critical Disorders; Chongqing Key Laboratory of Pediatrics 
      Chongqing, 400014, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Argonaute Proteins)
RN  - 0 (PIWIL2 protein, human)
SB  - IM
MH  - Argonaute Proteins
MH  - Cell Movement/*physiology
MH  - Cell Proliferation/*physiology
MH  - Exosomes/*physiology
MH  - Fetal Blood/*cytology
MH  - Humans
MH  - Karyotyping
MH  - Mesenchymal Stem Cells/*pathology
MH  - Neoplasm Invasiveness
MH  - *Neoplastic Stem Cells
MH  - Umbilical Cord
MH  - Wound Healing
PMC - PMC6744204
OTO - NOTNLM
OT  - cancer stem cells
OT  - exosomes
OT  - human umbilical cord blood-derived mesenchymal stem cells
OT  - invasion
OT  - migration
OT  - proliferation
EDAT- 2019/01/08 06:00
MHDA- 2019/02/23 06:00
PMCR- 2018/12/20
CRDT- 2019/01/08 06:00
PHST- 2019/01/08 06:00 [entrez]
PHST- 2019/01/08 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
PHST- 2018/12/20 00:00 [pmc-release]
AID - nfykdxxb-38-12-1440 [pii]
AID - 10.12122/j.issn.1673-4254.2018.12.07 [doi]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2018 Dec 30;38(12):1440-1447. doi: 
      10.12122/j.issn.1673-4254.2018.12.07.