PMID- 30613435
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220331
IS  - 2155-9570 (Print)
IS  - 2155-9570 (Electronic)
VI  - 9
IP  - 5
DP  - 2018
TI  - Disintegrin-Integrin Binding for Attachment of Polymer Substrate to the Retina.
LID - 752 [pii]
LID - 10.4172/2155-9570.1000752 [doi]
AB  - OBJECTIVE: We propose a novel attachment method for retinal tissue that utilizes 
      silicone modified with bioactive molecules. DESIGN: This is an experimental study 
      divided into an in vitro section performed in cadaveric pig eyes and an in vivo 
      section performed in rabbits. SUBJECTS: During in vitro experiments 36 cadaveric 
      pig eyes were used. During in vivo experiments 4 rabbits were used. METHODS: 
      Different types of silicone went through a laser irradiation process to determine 
      if binding sites for disintegrins could be created. Laser treated silicones that 
      showed disintegrin binding were evaluated with in vitro testing for 
      retina-silicone attachment. The best silicone binding in vitro was implanted into 
      a rabbit's eye after a full vitrectomy was performed. Post-operative exams were 
      done every two weeks to evaluate placement, attachment and sterilization method. 
      After three months animals were euthanized and eye was enucleated for histology 
      analysis. MAIN OUTCOME MEASURES: Attachment strength between 
      silicone-disintegrin-retina, and signs of endophthalmitis during in vivo studies 
      for biocompatibility purposes. RESULTS: A technique to successfully lase and 
      produce an active area on the silicone surface was described. Scanning electron 
      microscope (SEM) images were evaluated to assess physical ablation/debris field 
      area on the surface, definition of edges, evenness, and symmetry of the lased 
      area allowing us to select MED 4800 silicone family for further testing. Cell 
      culture experiments showed disintegrin binding to the silicone active area. In 
      vitro experiments with cadaveric eyes were performed to test retina-silicone 
      attachment. MED 4860 showed strongest attachment to the retina and it was used 
      during in vivo experiments. A sterilization protocol was tested and proved to be 
      reliable for bioactive materials. Disintegrin coated silicone showed attachment 
      in 2 of 4 rabbits during the 3-month implant period. The adhesion was persistent 
      until reversed with plasmin. All rabbits were implanted for 3 months regardless 
      of attachment, to test the feasibility of the sterilization method. None of the 
      rabbits developed any type of eye infection during the implant period. 
      CONCLUSION: We successfully lased and produced an active area on the silicone 
      surface to allow disintegrin-silicone binding. Different silicones interact 
      differently with the laser energy, and this is reflected in the strength of the 
      silicone-disintegrin-retina attachment.
FAU - Gonzalez-Calle, Alejandra
AU  - Gonzalez-Calle A
AD  - Dr Allen and Charlotte Ginsburg Institute for Biomedical Therapeutics, USC Roski 
      Eye Institute, University of Southern California, United States.
FAU - Brant, Rodrigo
AU  - Brant R
AD  - Dr Allen and Charlotte Ginsburg Institute for Biomedical Therapeutics, USC Roski 
      Eye Institute, University of Southern California, United States.
AD  - Department of Ophthalmology and Visual Sciences, Federal University of Sao Paulo, 
      Brazil.
FAU - Diniz, Bruno
AU  - Diniz B
AD  - Dr Allen and Charlotte Ginsburg Institute for Biomedical Therapeutics, USC Roski 
      Eye Institute, University of Southern California, United States.
FAU - Swenson, Steven
AU  - Swenson S
AD  - Department of Biochemistry and Molecular Medicine, Keck School of Medicine, 
      University of Southern California, United States of America.
AD  - Department of Neurological Surgery, Keck School of Medicine, University of 
      Southern California, United States of America.
FAU - Markland, Frank
AU  - Markland F
AD  - Department of Biochemistry and Molecular Medicine, Keck School of Medicine, 
      University of Southern California, United States of America.
FAU - Humayun, Mark S
AU  - Humayun MS
AD  - Dr Allen and Charlotte Ginsburg Institute for Biomedical Therapeutics, USC Roski 
      Eye Institute, University of Southern California, United States.
FAU - Weiland, James D
AU  - Weiland JD
AD  - Department of Biomedical Engineering and Ophthalmology, University of Michigan, 
      United States of America.
LA  - eng
GR  - RC3 EY020790/EY/NEI NIH HHS/United States
PT  - Journal Article
DEP - 20181008
PL  - United States
TA  - J Clin Exp Ophthalmol
JT  - Journal of clinical & experimental ophthalmology
JID - 101558412
PMC - PMC6319904
MID - NIHMS996133
OTO - NOTNLM
OT  - Disintegrin integrin
OT  - Method vicrostatin Retina
OT  - Novel attachment
OT  - Retinal prostheses
OT  - Visual impairment
EDAT- 2019/01/08 06:00
MHDA- 2019/01/08 06:01
PMCR- 2019/01/04
CRDT- 2019/01/08 06:00
PHST- 2019/01/08 06:00 [entrez]
PHST- 2019/01/08 06:00 [pubmed]
PHST- 2019/01/08 06:01 [medline]
PHST- 2019/01/04 00:00 [pmc-release]
AID - 752 [pii]
AID - 10.4172/2155-9570.1000752 [doi]
PST - ppublish
SO  - J Clin Exp Ophthalmol. 2018;9(5):752. doi: 10.4172/2155-9570.1000752. Epub 2018 
      Oct 8.