PMID- 30614543
OWN - NLM
STAT- MEDLINE
DCOM- 20200519
LR  - 20200519
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 59
IP  - 4
DP  - 2019 Apr
TI  - Stored red blood cells enhance in vivo migration of dendritic cells by promoting 
      reactive oxygen species-induced cytoskeletal rearrangement.
PG  - 1312-1323
LID - 10.1111/trf.15123 [doi]
AB  - BACKGROUND: A complex array of physicochemical changes occurs in red blood cells 
      (RBCs) during storage, leading to enhanced posttransfusion clearance. Dendritic 
      cells (DCs) play crucial roles in the engulfment of aged RBCs; however, it is 
      unclear how stored RBCs (sRBCs) modulate their responses to inflammatory stimuli 
      and DC migration ability. STUDY DESIGN AND METHODS: In this study, we examined 
      whether sRBCs affect the migration ability of DCs and elucidated the detailed 
      mechanisms mediating this process. Murine RBCs were incubated with marrow DCs 
      after removing the storage supernatant. The effects of sRBCs on cytokine 
      secretion from DCs, surface marker expression, and homing ability were examined. 
      RESULTS: More sRBCs were internalized by DCs than fresh RBCs (fRBCs), and RBC 
      accumulation significantly promoted the expression of allostimulatory molecules 
      and the secretion of Th1-type cytokines in the presence of lipopolysaccharide 
      (LPS). In particular, the lymphoid-tissue homing ability of transfused DCs 
      treated with sRBCs (sRBC-DCs) was also significantly greater than that of fRBCs. 
      Up regulation of CCR7 and improved organization of the cytoskeleton were observed 
      in sRBC-DCs, and blocking Rho/Rho-associated protein kinase (ROCK), PI3K/Akt, and 
      NF-kappaB pathways greatly hindered cytoskeletal rearrangement. Moreover, high levels 
      of reactive oxygen species (ROS) were detected in sRBC-DCs, and treatment with 
      N-acetylcysteine simultaneously decreased the lymph node-homing ability of DCs 
      and phosphorylation of RhoA, ROCK1, and cortactin. CONCLUSIONS: sRBCs initiated 
      differential immune responses compared to fRBCs, and the presence of LPS 
      augmented this phenomenon. Up regulation of CCR7 and ROS production promotes 
      cytoskeletal reorganization and contributes to the increased homing of sRBCs-DCs.
CI  - (c) 2019 AABB.
FAU - Zhao, Man
AU  - Zhao M
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
AD  - Department of Blood Transfusion, Chinese PLA General Hospital, Beijing, China.
FAU - Zhou, Qianqian
AU  - Zhou Q
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
FAU - He, Chulin
AU  - He C
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
FAU - Zhang, Yulong
AU  - Zhang Y
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
FAU - Wang, Zhengjun
AU  - Wang Z
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
FAU - Cai, Ruiying
AU  - Cai R
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
FAU - Ma, Cong
AU  - Ma C
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
FAU - Li, Yuan
AU  - Li Y
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
FAU - Wang, Xiaohui
AU  - Wang X
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
FAU - Zhan, Linsheng
AU  - Zhan L
AD  - Beijing Key Laboratory of Blood Safety and Security, Institute of Health Service 
      and Transfusion Medicine, Beijing, P.R. China.
AD  - Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, 
      Zhengzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190107
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Animals
MH  - *Blood Preservation
MH  - Cell Movement/*immunology
MH  - Cytoskeleton/*immunology
MH  - Dendritic Cells/cytology/*immunology
MH  - Erythrocytes/cytology/*immunology
MH  - Gene Expression Regulation/immunology
MH  - Male
MH  - Mice
MH  - Reactive Oxygen Species/*immunology
MH  - Signal Transduction/immunology
EDAT- 2019/01/08 06:00
MHDA- 2020/05/20 06:00
CRDT- 2019/01/08 06:00
PHST- 2018/04/12 00:00 [received]
PHST- 2018/10/27 00:00 [revised]
PHST- 2018/10/28 00:00 [accepted]
PHST- 2019/01/08 06:00 [pubmed]
PHST- 2020/05/20 06:00 [medline]
PHST- 2019/01/08 06:00 [entrez]
AID - 10.1111/trf.15123 [doi]
PST - ppublish
SO  - Transfusion. 2019 Apr;59(4):1312-1323. doi: 10.1111/trf.15123. Epub 2019 Jan 7.