PMID- 30615852
OWN - NLM
STAT- MEDLINE
DCOM- 20191213
LR  - 20201019
IS  - 1096-0384 (Electronic)
IS  - 0003-9861 (Linking)
VI  - 663
DP  - 2019 Mar 15
TI  - Generation and characterization of antagonistic anti-human interleukin (IL)-18 
      monoclonal antibodies with high affinity: Two types of monoclonal antibodies 
      against full-length IL-18 and the neoepitope of inflammatory caspase-cleaved 
      active IL-18.
PG  - 71-82
LID - S0003-9861(18)31013-0 [pii]
LID - 10.1016/j.abb.2019.01.001 [doi]
AB  - Interleukin-18 (IL-18) is a pro-inflammatory cytokine that evokes both innate and 
      acquired immune responses. IL-18 is initially synthesized as an inactive 
      precursor and the cleavage for processing into a mature, active molecule is 
      mediated by pro-inflammatory caspases following the activation of inflammasomes. 
      Two types of monoclonal antibodies were raised: anti-IL-18(63-68) antibodies 
      which recognize full-length(1-193) and cleaved IL-18; and anti-IL-18 neoepitope 
      antibodies which specifically recognize the new N-terminal (37)YFGKLESK(44) of 
      IL-18 cleaved by pro-inflammatory caspase-1/4. These mAbs were suitable for 
      Western blotting, capillary Western immunoassay (WES), immunofluorescence, 
      immunoprecipitation, and function-blocking assays. WES analysis of these mAbs 
      allowed visualization of the IL-18 bands and provided a molecular weight 
      corresponding to the pro-inflammatory caspase-1/4 cleaved, active form 
      IL-18(37-193), and not to the inactive precursor IL-18, in the serum of patients 
      with adult-onset Still's disease (6/14, 42%) and hemophagocytic activation 
      syndrome (2/6, 33%). These monoclonal antibodies will be very useful in IL-18 and 
      inflammasome biology and for diagnostic and therapeutic strategies for 
      inflammatory diseases.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Nariai, Yuko
AU  - Nariai Y
AD  - Department of Biochemistry, Shimane University School of Medicine, Izumo, 
      693-8501, Japan.
FAU - Kamino, Hiroki
AU  - Kamino H
AD  - Department of Biochemistry, Shimane University School of Medicine, Izumo, 
      693-8501, Japan.
FAU - Obayashi, Eiji
AU  - Obayashi E
AD  - Department of Biochemistry, Shimane University School of Medicine, Izumo, 
      693-8501, Japan.
FAU - Kato, Hiroaki
AU  - Kato H
AD  - Department of Biochemistry, Shimane University School of Medicine, Izumo, 
      693-8501, Japan.
FAU - Sakashita, Gyosuke
AU  - Sakashita G
AD  - Department of Biochemistry, Shimane University School of Medicine, Izumo, 
      693-8501, Japan.
FAU - Sugiura, Tomoko
AU  - Sugiura T
AD  - Department of Biochemistry, Shimane University School of Medicine, Izumo, 
      693-8501, Japan.
FAU - Migita, Kiyoshi
AU  - Migita K
AD  - Department of Rheumatology, Fukushima Medical University School of Medicine, 
      Fukushima, 960-1247, Japan.
FAU - Koga, Tomohiro
AU  - Koga T
AD  - Department of Rheumatology, Unit of Advanced Preventive Medical Sciences, 
      Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8501, 
      Japan.
FAU - Kawakami, Atsushi
AU  - Kawakami A
AD  - Department of Rheumatology, Unit of Advanced Preventive Medical Sciences, 
      Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, 852-8501, 
      Japan.
FAU - Sakamoto, Kazuma
AU  - Sakamoto K
AD  - Department of Biochemistry, Nagoya University Graduate School of Medicine, 65 
      Tsurumai-Cho, Showa-Ku, Nagoya, 466-8550, Japan.
FAU - Kadomatsu, Kenji
AU  - Kadomatsu K
AD  - Department of Biochemistry, Nagoya University Graduate School of Medicine, 65 
      Tsurumai-Cho, Showa-Ku, Nagoya, 466-8550, Japan.
FAU - Nakakido, Makoto
AU  - Nakakido M
AD  - Department of Bioengineering, School of Engineering, The University of Tokyo, 
      Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.
FAU - Tsumoto, Kouhei
AU  - Tsumoto K
AD  - Department of Bioengineering, School of Engineering, The University of Tokyo, 
      Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan; Institute of Medical Science, The 
      University of Tokyo, Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan.
FAU - Urano, Takeshi
AU  - Urano T
AD  - Department of Biochemistry, Shimane University School of Medicine, Izumo, 
      693-8501, Japan; mAbProtein Co. Ltd, Izumo, 693-8501, Japan. Electronic address: 
      turano@med.shimane-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190104
PL  - United States
TA  - Arch Biochem Biophys
JT  - Archives of biochemistry and biophysics
JID - 0372430
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (IL18 protein, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-18)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Antibodies, Monoclonal/*biosynthesis/*immunology
MH  - Antibody Affinity
MH  - Caspases/*metabolism
MH  - Cell Line, Tumor
MH  - HEK293 Cells
MH  - Humans
MH  - Inflammation Mediators/*immunology/metabolism
MH  - Interferon-gamma/metabolism
MH  - Interleukin-18/*immunology/metabolism
MH  - Proteolysis
EDAT- 2019/01/08 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/01/08 06:00
PHST- 2018/12/11 00:00 [received]
PHST- 2018/12/29 00:00 [revised]
PHST- 2019/01/03 00:00 [accepted]
PHST- 2019/01/08 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/01/08 06:00 [entrez]
AID - S0003-9861(18)31013-0 [pii]
AID - 10.1016/j.abb.2019.01.001 [doi]
PST - ppublish
SO  - Arch Biochem Biophys. 2019 Mar 15;663:71-82. doi: 10.1016/j.abb.2019.01.001. Epub 
      2019 Jan 4.