PMID- 30618630
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240105
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 12
DP  - 2018
TI  - 1, 25-D(3) Protects From Cerebral Ischemia by Maintaining BBB Permeability via 
      PPAR-gamma Activation.
PG  - 480
LID - 10.3389/fncel.2018.00480 [doi]
LID - 480
AB  - The blood-brain barrier (BBB) is a physical and biochemical barrier that 
      maintains cerebral homeostasis. BBB dysfunction in an ischemic stroke, results in 
      brain injury and subsequent neurological impairment. The aim of this study was to 
      determine the possible protective effects of 1, 25-dihydroxyvitamin D(3) [1, 
      25(OH)(2)D(3), 1, 25-D(3), vit D] on BBB dysfunction, at the early stages of an 
      acute ischemic brain injury. We analyzed the effects of 1, 25-D(3) on BBB 
      integrity in terms of histopathological changes, the neurological deficit, 
      infarct size and the expression of brain derived neurotrophic factor (BDNF), in a 
      middle cerebral artery occlusion/reperfusion (MCAO/R) rat model. BBB permeability 
      and the expression of permeability-related proteins in the brain were also 
      evaluated by Evans blue (EB) staining and Western blotting respectively. To 
      determine the possible mechanism underlying the role of 1, 25-D(3) in BBB 
      maintenance, after MCAO/R, the rats were treated with the specific peroxisome 
      proliferator-activated receptor gamma (PPARgamma) inhibitor GW9662. Supplementation 
      with 1, 25-D(3) markedly improved the neurological scores of the rats, decreased 
      the infarct volume, prevented neuronal deformation and upregulated the expression 
      of the tight junction (TJ) and BDNF proteins in their brains. Furthermore, it 
      activated PPARgamma but downregulated neuro-inflammatory cytokines such as nuclear 
      factor kappa-B (NF-kappaB) and tumor necrosis factor-alpha (TNF-alpha), after MCAO/R. Taken 
      together, 1, 25-D(3) protects against cerebral ischemia by maintaining BBB 
      permeability, upregulating the level of BDNF and inhibiting PPARgamma-mediated 
      neuro-inflammation.
FAU - Guo, Ting
AU  - Guo T
AD  - Department of Neurology, Xuzhou Medical University, Xuzhou, China.
FAU - Wang, Yanqiang
AU  - Wang Y
AD  - Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, 
      Xuzhou, China.
AD  - Department of Neurology, The Affiliated Hospital of Weifang Medical University, 
      Weifang, China.
FAU - Guo, Yuanfang
AU  - Guo Y
AD  - Department of Respiratory Medicine, Ganyu District People's Hospital, 
      Lianyungang, China.
FAU - Wu, Shuguang
AU  - Wu S
AD  - Department of Anesthesiology, The Second Affiliated Hospital of Zhejiang 
      University School of Medicine, Hangzhou, China.
FAU - Chen, Weiwei
AU  - Chen W
AD  - Department of Neurology, The Central Hospital of Xuzhou, Xuzhou, China.
FAU - Liu, Na
AU  - Liu N
AD  - Department of Neurology, Xuzhou Medical University, Xuzhou, China.
FAU - Wang, Yu
AU  - Wang Y
AD  - Department of Neurology, Xuzhou Medical University, Xuzhou, China.
FAU - Geng, Deqin
AU  - Geng D
AD  - Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, 
      Xuzhou, China.
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20181217
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
RIN - Front Cell Neurosci. 2023 Dec 21;17:1355800. PMID: 38179206
PMC - PMC6304345
OTO - NOTNLM
OT  - 1
OT  - 25-D3
OT  - BBB permeability
OT  - BDNF
OT  - MCAO/R
OT  - NF-kappaB
OT  - PPARgamma
OT  - inflammation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2019/01/09 06:00
MHDA- 2019/01/09 06:01
PMCR- 2018/01/01
CRDT- 2019/01/09 06:00
PHST- 2018/09/26 00:00 [received]
PHST- 2018/11/23 00:00 [accepted]
PHST- 2019/01/09 06:00 [entrez]
PHST- 2019/01/09 06:00 [pubmed]
PHST- 2019/01/09 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2018.00480 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2018 Dec 17;12:480. doi: 10.3389/fncel.2018.00480. 
      eCollection 2018.