PMID- 30618730
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 9
DP  - 2018
TI  - MiR-3188 Inhibits Non-small Cell Lung Cancer Cell Proliferation Through 
      FOXO1-Mediated mTOR-p-PI3K/AKT-c-JUN Signaling Pathway.
PG  - 1362
LID - 10.3389/fphar.2018.01362 [doi]
LID - 1362
AB  - This study investigated the role of miR-3188 on the proliferation of non-small 
      cell lung cancer cells and its relationship to FOXO1-modulated feedback loop. Two 
      non-small cell lung cancer (NSCLC) cell lines A549 and H1299 were used. RNA 
      silencing was achieved by lentiviral transfection. Cell proliferation was 
      assessed by immunohistochemical staining of Ki67 and PCNA, Edu incorporation, and 
      colony formation assay. Western blotting was used to examine expression of FOXO1, 
      mTOR, p-mTOR, CCND1, p21, c-JUN, AKT, pAKT, PI3K, p-PI3K, and p27 proteins. It 
      was found that miR-3188 reduced cell proliferation in NSCLC cells. Molecular 
      analyses indicated that the effect of mammalian target of rapamycin (mTOR) was 
      directly mediated by miR-3188, leading to p-PI3K/p-AKT/c-JUN inactivation. The 
      inhibition of this signaling pathway further caused cell-cycle suppression. 
      Moreover, FOXO1 was found to be involved in regulating the interaction of 
      miR-3188 and mTOR through p-PI3K/p-AKT/c-JUN signaling pathway. Taken together, 
      our study demonstrated that miR-3188 interacts with mTOR and FOXO1 to inhibit 
      NSCLC cell proliferation through a mTOR-p-PI3K/AKT-c-JUN signaling pathway. 
      Therefore, miR-3188 might be a potential target for the treatment of NSCLC.
FAU - Wang, Chunyan
AU  - Wang C
AD  - College of Food and Biology Engineering, Xuzhou Institute of Technology, Xuzhou, 
      China.
FAU - Liu, Enqi
AU  - Liu E
AD  - College of Food and Biology Engineering, Xuzhou Institute of Technology, Xuzhou, 
      China.
FAU - Li, Wen
AU  - Li W
AD  - College of Food and Biology Engineering, Xuzhou Institute of Technology, Xuzhou, 
      China.
FAU - Cui, Jue
AU  - Cui J
AD  - College of Food and Biology Engineering, Xuzhou Institute of Technology, Xuzhou, 
      China.
FAU - Li, Tongxiang
AU  - Li T
AD  - College of Food and Biology Engineering, Xuzhou Institute of Technology, Xuzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20181211
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC6297856
OTO - NOTNLM
OT  - NSCLC
OT  - PI3K/AKT
OT  - c-JUN
OT  - mTOR
OT  - miR-3188
OT  - proliferation
EDAT- 2019/01/09 06:00
MHDA- 2019/01/09 06:01
PMCR- 2018/12/11
CRDT- 2019/01/09 06:00
PHST- 2018/09/19 00:00 [received]
PHST- 2018/11/05 00:00 [accepted]
PHST- 2019/01/09 06:00 [entrez]
PHST- 2019/01/09 06:00 [pubmed]
PHST- 2019/01/09 06:01 [medline]
PHST- 2018/12/11 00:00 [pmc-release]
AID - 10.3389/fphar.2018.01362 [doi]
PST - epublish
SO  - Front Pharmacol. 2018 Dec 11;9:1362. doi: 10.3389/fphar.2018.01362. eCollection 
      2018.