PMID- 30619352
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20200309
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - Shrimp miR-1000 Functions in Antiviral Immunity by Simultaneously Triggering the 
      Degradation of Two Viral mRNAs.
PG  - 2999
LID - 10.3389/fimmu.2018.02999 [doi]
LID - 2999
AB  - MicroRNAs (miRNAs) function as crucial suppressors of gene expression via 
      translational repression or direct mRNA degradation. However, the mechanism of 
      multi-gene regulation by a host miRNA in antiviral immunity has not been 
      extensively explored. In this study, the regulation of two white spot syndrome 
      virus (WSSV) genes by its host (Marsupenaeus japonicus shrimp) miRNA (shrimp 
      miR-1000) was characterized. The miRNA target gene prediction showed that only 
      two virus genes (wsv191 and wsv407) might be the targets of miR-1000. The results 
      of insect cell transfection assays revealed that shrimp miR-1000 could target 
      multiple virus genes (wsv191 and wsv407). The mRNA degradation analysis and RNA 
      FISH (fluorescence in situ hybridization) analysis indicated that miR-1000 
      triggered the mRNA degradation of target genes through 5'-3' exonucleolytic 
      digestion in vivo and thereby inhibited the virus infection in shrimp. The 
      miRNA-mediated 5'-3' exonucleolytic digestion of target mRNAs stopped near the 
      3'UTR (3'untranslated region) sequence complementary to the seed sequence of 
      miR-1000. Therefore, our study provided novel insights into how a host miRNA 
      targeted multiple viral genes and prevented host from virus infection.
FAU - Gong, Yi
AU  - Gong Y
AD  - Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory 
      for Marine Science and Technology, College of Life Sciences, Zhejiang University, 
      Hangzhou, China.
FAU - Ju, Chenyu
AU  - Ju C
AD  - Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory 
      for Marine Science and Technology, College of Life Sciences, Zhejiang University, 
      Hangzhou, China.
FAU - Zhang, Xiaobo
AU  - Zhang X
AD  - Laboratory for Marine Biology and Biotechnology of Qingdao National Laboratory 
      for Marine Science and Technology, College of Life Sciences, Zhejiang University, 
      Hangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181218
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Genes, Viral
MH  - Host-Pathogen Interactions/*genetics/immunology
MH  - MicroRNAs/*immunology/metabolism
MH  - Penaeidae/genetics/*immunology/virology
MH  - RNA, Messenger/metabolism
MH  - RNA, Viral/metabolism
MH  - Up-Regulation
MH  - Virus Diseases/genetics/*immunology/veterinary/virology
MH  - White spot syndrome virus 1/genetics/*immunology
PMC - PMC6305465
OTO - NOTNLM
OT  - mRNA degradation
OT  - shrimp miR-1000
OT  - virus-host interactions
OT  - white spot syndrome virus gene
OT  - wsv191 and wsv407
EDAT- 2019/01/09 06:00
MHDA- 2019/11/26 06:00
PMCR- 2018/01/01
CRDT- 2019/01/09 06:00
PHST- 2018/06/01 00:00 [received]
PHST- 2018/12/04 00:00 [accepted]
PHST- 2019/01/09 06:00 [entrez]
PHST- 2019/01/09 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.02999 [doi]
PST - epublish
SO  - Front Immunol. 2018 Dec 18;9:2999. doi: 10.3389/fimmu.2018.02999. eCollection 
      2018.