PMID- 30619364
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20200309
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - Dual Regulation of Host TRAIP Post-translation and Nuclear/Plasma Distribution by 
      Porcine Reproductive and Respiratory Syndrome Virus Non-structural Protein 1alpha 
      Promotes Viral Proliferation.
PG  - 3023
LID - 10.3389/fimmu.2018.03023 [doi]
LID - 3023
AB  - In this study, we show that porcine reproductive and respiratory syndrome virus 
      (PRRSV) non-structural protein 1alpha (nsp1alpha) facilitates PRRSV escape from innate 
      immune by modulating nuclear to cytoplasmic translocation and distribution ratio 
      of TRAIP to promote virus proliferation. Mechanistically, TRAIP interacts with 
      PRRSV nsp1alpha via its K205 site, while NSP1alpha decreases the SUMOylation and K48 
      ubiquitination independent of the TRAIP interaction K205 site. Modulation of the 
      dual modification of TRAIP by PRRSV nsp1alpha results in over-enrichment of TRAIP in 
      the cytoplasm. Enrichment of nsp1alpha-induced cytoplasmic TRAIP in turn leads to 
      excessive K48 ubiquitination and degradation of serine/threonine-protein kinase 
      (TBK1), thereby antagonizing TBK1-IRF3-IFN signaling. This study proposes a novel 
      mechanism by which PRRSV utilizes host proteins to regulate innate immunity. 
      Findings from this study provides novel perspective to advance our understanding 
      in the pathogenesis of PRRSV.
FAU - Shi, Peidian
AU  - Shi P
AD  - School of Life Sciences, Tianjin University, Tianjin, China.
FAU - Su, Yanxin
AU  - Su Y
AD  - School of Life Sciences, Tianjin University, Tianjin, China.
FAU - Li, Ruiqiao
AU  - Li R
AD  - School of Life Sciences, Tianjin University, Tianjin, China.
FAU - Zhang, Lei
AU  - Zhang L
AD  - School of Life Sciences, Tianjin University, Tianjin, China.
FAU - Chen, Chen
AU  - Chen C
AD  - School of Life Sciences, Tianjin University, Tianjin, China.
FAU - Zhang, Lilin
AU  - Zhang L
AD  - School of Life Sciences, Tianjin University, Tianjin, China.
FAU - Faaberg, Kay
AU  - Faaberg K
AD  - Agricultural Research Service, USDA, National Animal Disease Center, Ames, IA, 
      United States.
FAU - Huang, Jinhai
AU  - Huang J
AD  - School of Life Sciences, Tianjin University, Tianjin, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181218
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Interferon Type I)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/metabolism
MH  - Cytoplasm/metabolism
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Host-Pathogen Interactions/*immunology
MH  - Humans
MH  - Immunity, Innate
MH  - Interferon Type I/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/pathogenicity
MH  - RNA, Small Interfering/metabolism
MH  - Sus scrofa
MH  - Swine
MH  - Ubiquitin-Protein Ligases/genetics/*metabolism
MH  - Ubiquitination/immunology
MH  - Viral Nonstructural Proteins/*immunology
MH  - Virus Replication/immunology
PMC - PMC6305329
OTO - NOTNLM
OT  - IFN
OT  - PRRSV
OT  - SUMOylation
OT  - TBK1
OT  - TRAIP
OT  - nsp1alpha
EDAT- 2019/01/09 06:00
MHDA- 2019/11/26 06:00
PMCR- 2018/01/01
CRDT- 2019/01/09 06:00
PHST- 2018/09/21 00:00 [received]
PHST- 2018/12/06 00:00 [accepted]
PHST- 2019/01/09 06:00 [entrez]
PHST- 2019/01/09 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.03023 [doi]
PST - epublish
SO  - Front Immunol. 2018 Dec 18;9:3023. doi: 10.3389/fimmu.2018.03023. eCollection 
      2018.