PMID- 30623524
OWN - NLM
STAT- MEDLINE
DCOM- 20190417
LR  - 20190417
IS  - 1365-2443 (Electronic)
IS  - 1356-9597 (Linking)
VI  - 24
IP  - 3
DP  - 2019 Mar
TI  - Cep128 associates with Odf2 to form the subdistal appendage of the centriole.
PG  - 231-243
LID - 10.1111/gtc.12668 [doi]
AB  - The mother centriole in a cell has two appendages, the distal appendage (DA) and 
      subdistal appendage (SDA), which have roles in generating cilia and organizing 
      the cellular microtubular network, respectively. In the knockout (KO) cells of 
      Odf2, the component of the DA and SDA, both appendages simultaneously disappear. 
      However, the molecular mechanisms by which the DA and SDA form independently but 
      close to each other downstream of Odf2 are unknown. Here, using super-resolution 
      structured illumination microscopy (SR-SIM), we found that the signal for 
      GFP-tagged Odf2 overlapped considerably with that of immunofluorescently labeled 
      Cep128. We further found that Cep128 knockdown (KD) caused the dissociation of 
      other SDA components from the centriole, including centriolin, Ndel1, ninein and 
      Cep170, whereas Odf2 was still associated with the centriole. In contrast, the DA 
      components remained associated with the centriole in Cep128 KD cells. Consistent 
      with this observation, we identified Cep128 as an Odf2-interacting protein by 
      immunoprecipitation. Taken with the finding that Cep128 deletion decreased the 
      stability of centriolar microtubules, our results indicate that Cep128 associates 
      with Odf2 in the hierarchical assembly of SDA components to elicit the 
      microtubule-organizing function.
CI  - (c) 2019 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
FAU - Kashihara, Hiroka
AU  - Kashihara H
AUID- ORCID: 0000-0002-6204-1348
AD  - Graduate School of Medicine, Osaka University, Osaka, Japan.
FAU - Chiba, Shuhei
AU  - Chiba S
AD  - Graduate School of Medicine, Osaka University, Osaka, Japan.
AD  - Graduate School of Medicine, Osaka City University, Osaka, Japan.
FAU - Kanno, Shin-Ichiro
AU  - Kanno SI
AD  - Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
FAU - Suzuki, Koya
AU  - Suzuki K
AD  - Graduate School of Medicine, Osaka University, Osaka, Japan.
FAU - Yano, Tomoki
AU  - Yano T
AD  - Graduate School of Medicine, Osaka University, Osaka, Japan.
FAU - Tsukita, Sachiko
AU  - Tsukita S
AD  - Graduate School of Medicine, Osaka University, Osaka, Japan.
LA  - eng
GR  - Joint Research Program of the Joint Usage/Research Center at the Institute of 
      Development, Aging and Cancer, Tohoku University/
GR  - 15H05596/Japan Society for the Promotion of Science/
GR  - 18H03999/Japan Society for the Promotion of Science/
GR  - 18J12466/Japan Society for the Promotion of Science/
GR  - 24247037/Japan Society for the Promotion of Science/
GR  - 11581/Core Research for Evolutional Science and Technology/
PT  - Journal Article
DEP - 20190218
PL  - England
TA  - Genes Cells
JT  - Genes to cells : devoted to molecular & cellular mechanisms
JID - 9607379
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (ODF2 protein, human)
SB  - IM
MH  - Centrioles/*metabolism
MH  - HEK293 Cells
MH  - Heat-Shock Proteins/genetics/*metabolism
MH  - Humans
MH  - Protein Binding
OTO - NOTNLM
OT  - Odf2
OT  - centrosome
OT  - microtubules
OT  - subdistal appendage
OT  - super-resolution structured illumination microscopy
EDAT- 2019/01/10 06:00
MHDA- 2019/04/18 06:00
CRDT- 2019/01/10 06:00
PHST- 2018/12/27 00:00 [received]
PHST- 2018/12/29 00:00 [accepted]
PHST- 2019/01/10 06:00 [pubmed]
PHST- 2019/04/18 06:00 [medline]
PHST- 2019/01/10 06:00 [entrez]
AID - 10.1111/gtc.12668 [doi]
PST - ppublish
SO  - Genes Cells. 2019 Mar;24(3):231-243. doi: 10.1111/gtc.12668. Epub 2019 Feb 18.