PMID- 30627118
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 9
DP  - 2018
TI  - Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote 
      Disease Progression and Induce the Production of Different Cytokines in 
      Macrophages and B-1 Cells.
PG  - 3056
LID - 10.3389/fmicb.2018.03056 [doi]
LID - 3056
AB  - The extracellular vesicles (EVs) released by Leishmania can contribute to the 
      establishment of infection and host immunomodulation. In this study, we 
      characterized the shedding of EVs from Leishmania (Leishmania) amazonensis 
      promastigotes. This species is the causative agent of cutaneous leishmaniasis, 
      and its role during interactions with bone marrow-derived macrophages (BMDMs) and 
      peritoneal B-1 cells was evaluated. Leishmania amazonensis promastigotes 
      cultivated in vitro at different times and temperatures spontaneously released 
      EVs. EVs were purified using size-exclusion chromatography (SEC) and quantitated 
      by nanoparticle tracking analysis (NTA). NTA revealed that the average size of 
      the EVs was approximately 180 nm, with concentrations ranging from 1.8 x 10(8) to 
      2.4 x 10(9) vesicles/mL. In addition, the presence of LPG and GP63 were detected 
      in EVs obtained at different temperatures. Naive BMDMs stimulated with EVs 
      exhibited increased IL-10 and IL-6 expression. However, incubating B-1 cells with 
      parasite EVs did not stimulate IL-10 expression but led to an increase in the 
      expression of IL-6 and TNFalpha. After 7 weeks post-infection, animals infected with 
      L. amazonensis promastigotes in the presence of parasite EVs had significant 
      higher parasite load and a polarization to Th2 response, as compared to the group 
      infected with the parasite alone. This work demonstrated that EVs isolated from 
      L. amazonensis promastigotes were able to stimulate macrophages and B-1 cells to 
      express different types of cytokines. Moreover, the immunomodulatory properties 
      of EVs probably contributed to an increase in parasite burden in mice. These 
      findings suggest that the functionality of L. amazonensis EVs on immune system 
      favor of parasite survival and disease progression.
FAU - Barbosa, Fernanda Marins Costa
AU  - Barbosa FMC
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Diadema, Brazil.
FAU - Dupin, Talita Vieira
AU  - Dupin TV
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Diadema, Brazil.
FAU - Toledo, Mayte Dos Santos
AU  - Toledo MDS
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Diadema, Brazil.
FAU - Reis, Natasha Ferraz Dos Campos
AU  - Reis NFDC
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Diadema, Brazil.
FAU - Ribeiro, Kleber
AU  - Ribeiro K
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Diadema, Brazil.
FAU - Cronemberger-Andrade, Andre
AU  - Cronemberger-Andrade A
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Diadema, Brazil.
FAU - Rugani, Jeronimo Nunes
AU  - Rugani JN
AD  - Instituto Rene Rachou/FIOCRUZ, Belo Horizonte, Brazil.
FAU - De Lorenzo, Beatriz Helena Pizarro
AU  - De Lorenzo BHP
AD  - Centro Universitario Sao Camilo, Sao Paulo, Brazil.
FAU - Novaes E Brito, Ronni Romulo
AU  - Novaes E Brito RR
AD  - Centro Universitario Sao Camilo, Sao Paulo, Brazil.
FAU - Soares, Rodrigo Pedro
AU  - Soares RP
AD  - Instituto Rene Rachou/FIOCRUZ, Belo Horizonte, Brazil.
FAU - Torrecilhas, Ana Claudia
AU  - Torrecilhas AC
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Diadema, Brazil.
FAU - Xander, Patricia
AU  - Xander P
AD  - Laboratorio de Imunologia Celular e Bioquimica de Fungos e Protozoarios, 
      Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo - 
      Campus Diadema, Diadema, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20181221
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6309564
OTO - NOTNLM
OT  - B-1 cells
OT  - L. amazonensis
OT  - cytokines
OT  - extracellular vesicles
OT  - macrophages
EDAT- 2019/01/11 06:00
MHDA- 2019/01/11 06:01
PMCR- 2018/12/21
CRDT- 2019/01/11 06:00
PHST- 2018/04/02 00:00 [received]
PHST- 2018/11/27 00:00 [accepted]
PHST- 2019/01/11 06:00 [entrez]
PHST- 2019/01/11 06:00 [pubmed]
PHST- 2019/01/11 06:01 [medline]
PHST- 2018/12/21 00:00 [pmc-release]
AID - 10.3389/fmicb.2018.03056 [doi]
PST - epublish
SO  - Front Microbiol. 2018 Dec 21;9:3056. doi: 10.3389/fmicb.2018.03056. eCollection 
      2018.