PMID- 30627557
OWN - NLM
STAT- MEDLINE
DCOM- 20190424
LR  - 20240109
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2018
DP  - 2018
TI  - Molecular Cytogenetics of Pisum sativum L. Grown under Spaceflight-Related 
      Stress.
PG  - 4549294
LID - 10.1155/2018/4549294 [doi]
LID - 4549294
AB  - The ontogenesis and reproduction of plants cultivated aboard a spacecraft occur 
      inside the unique closed ecological system wherein plants are subjected to 
      serious abiotic stresses. For the first time, a comparative molecular cytogenetic 
      analysis of Pisum sativum L. (Fabaceae) grown on board the RS ISS during the 
      Expedition-14 and Expedition-16 and also plants of their succeeding (F1 and F2) 
      generations cultivated on Earth was performed in order to reveal possible 
      structural chromosome changes in the pea genome. The karyotypes of these plants 
      were studied by multicolour fluorescence in situ hybridization (FISH) with five 
      different repeated DNA sequences (45S rDNA, 5S rDNA, PisTR-B/1, microsatellite 
      motifs (AG)(12), and (GAA)(9)) as probes. A chromosome aberration was revealed in 
      one F1 plant. Significant changes in distribution of the examined repeated DNAs 
      in karyotypes of the "space grown" pea plants as well as in F1 and F2 plants 
      cultivated on Earth were not observed if compared with control plants. Additional 
      oligo-(GAA)(9) sites were detected on chromosomes 6 and 7 in karyotypes of F1 and 
      F2 plants. The detected changes might be related to intraspecific genomic 
      polymorphism or plant cell adaptive responses to spaceflight-related stress 
      factors. Our findings suggest that, despite gradual total trace contamination of 
      the atmosphere on board the ISS associated with the extension of the space 
      station operating life, exposure to the space environment did not induce serious 
      chromosome reorganizations in genomes of the "space grown" pea plants and 
      generations of these plants cultivated on Earth.
FAU - Yurkevich, Olga Yu
AU  - Yurkevich OY
AUID- ORCID: 0000-0003-4124-2367
AD  - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 
      Moscow, Russia.
FAU - Samatadze, Tatiana E
AU  - Samatadze TE
AD  - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 
      Moscow, Russia.
FAU - Levinskikh, Margarita A
AU  - Levinskikh MA
AD  - Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, 
      Russia.
FAU - Zoshchuk, Svyatoslav A
AU  - Zoshchuk SA
AD  - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 
      Moscow, Russia.
FAU - Signalova, Olga B
AU  - Signalova OB
AD  - Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, 
      Russia.
FAU - Surzhikov, Sergei A
AU  - Surzhikov SA
AD  - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 
      Moscow, Russia.
FAU - Sychev, Vladimir N
AU  - Sychev VN
AD  - Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, 
      Russia.
FAU - Amosova, Alexandra V
AU  - Amosova AV
AUID- ORCID: 0000-0002-5208-6702
AD  - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 
      Moscow, Russia.
FAU - Muravenko, Olga V
AU  - Muravenko OV
AUID- ORCID: 0000-0003-3150-8598
AD  - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 
      Moscow, Russia.
LA  - eng
PT  - Journal Article
DEP - 20181206
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Chromosome Aberrations
MH  - Chromosomes, Plant/genetics
MH  - Cytogenetics/methods
MH  - DNA, Ribosomal/genetics
MH  - Karyotype
MH  - Karyotyping/methods
MH  - Microsatellite Repeats/genetics
MH  - Pisum sativum/*genetics
MH  - Space Flight/methods
MH  - Stress, Physiological/*genetics
PMC - PMC6304655
EDAT- 2019/01/11 06:00
MHDA- 2019/04/25 06:00
PMCR- 2018/12/06
CRDT- 2019/01/11 06:00
PHST- 2018/09/21 00:00 [received]
PHST- 2018/10/26 00:00 [revised]
PHST- 2018/11/22 00:00 [accepted]
PHST- 2019/01/11 06:00 [entrez]
PHST- 2019/01/11 06:00 [pubmed]
PHST- 2019/04/25 06:00 [medline]
PHST- 2018/12/06 00:00 [pmc-release]
AID - 10.1155/2018/4549294 [doi]
PST - epublish
SO  - Biomed Res Int. 2018 Dec 6;2018:4549294. doi: 10.1155/2018/4549294. eCollection 
      2018.