PMID- 30627566 OWN - NLM STAT- MEDLINE DCOM- 20190425 LR - 20200225 IS - 2314-6141 (Electronic) IS - 2314-6133 (Print) VI - 2018 DP - 2018 TI - Mass Spectrometry-Based Comprehensive Analysis of Pancreatic Cyst Fluids. PG - 7169595 LID - 10.1155/2018/7169595 [doi] LID - 7169595 AB - Pancreatic cyst fluids (PCFs) enriched in tumour-derived proteins are considered a potential source of new biomarkers. This study aimed to determine compositional and quantitative differences between the degradome and proteome of PCFs aspirated from different types of pancreatic cyst lesions (PCLs). 91 patients who underwent endoscopic ultrasound-fine needle aspiration under routine clinical diagnosis of PCLs were enrolled. Four cysts were malignant (CAs), and 87 were nonmalignant and consisted of 18 intraductal papillary mucinous neoplasms (IPMNs), 14 mucinous cystic neoplasms (MCNs), nine serous cystic neoplasms (SCNs), 29 pseudocysts (PCs), and 17 unclassified. Profiles of the <5 kDa fraction, the degradome, and the trypsin-digested proteome were analysed using an LTQ-Orbitrap Elite mass spectrometer coupled with a nanoACQUITY LC system. Qualitative analyses identified 796 and 366 proteins in degradome and proteome, respectively, and 689 (77%) and 285 (78%) of them were present in the Plasma Proteome Database. Gene Ontology analysis showed a significant overrepresentation of peptidases and peptidases inhibitors in both datasets. In the degradome fraction, quantitative values were obtained for 6996 peptides originating from 657 proteins. Of these, 2287 peptides were unique to a single type, and 515 peptides, derived from 126 proteins, were shared across cyst types. 32 peptides originating from 12 proteins had differential (adjusted p-value /=1.5) abundance in at least one of the five cysts types. In proteome, relative expression was measured for 330 proteins. Of them, 33 proteins had significantly (adjusted p-value /=1.5) altered abundance in at least one of the studied groups and 19 proteins appeared to be unique to a given cyst type. PCFs are dominated by blood proteins and proteolytic enzymes. Although differences in PCF peptide composition and abundance could aid classification of PCLs, the unpredictable inherent PCF proteolytic activity may limit the practical applications of PCF protein profiling. FAU - Paziewska, Agnieszka AU - Paziewska A AD - Department of Gastroenterology, Hepatology and Clinical Oncology, Medical Center for Postgraduate Education, Warsaw, Poland. FAU - Polkowski, Marcin AU - Polkowski M AD - Department of Gastroenterology, Hepatology and Clinical Oncology, Medical Center for Postgraduate Education, Warsaw, Poland. FAU - Rubel, Tymon AU - Rubel T AD - Institute of Radioelectronics and Multimedia Technology, Warsaw University of Technology, Warsaw, Poland. FAU - Karczmarski, Jakub AU - Karczmarski J AD - Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. FAU - Wiechowska-Kozlowska, Anna AU - Wiechowska-Kozlowska A AD - Department of Endoscopy, Ministry of Internal Affairs Hospital, Szczecin, Poland. FAU - Dabrowska, Michalina AU - Dabrowska M AD - Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. FAU - Mikula, Michal AU - Mikula M AUID- ORCID: 0000-0003-3447-7328 AD - Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. FAU - Dadlez, Michal AU - Dadlez M AD - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland. FAU - Ostrowski, Jerzy AU - Ostrowski J AUID- ORCID: 0000-0002-1450-8564 AD - Department of Gastroenterology, Hepatology and Clinical Oncology, Medical Center for Postgraduate Education, Warsaw, Poland. AD - Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland. LA - eng PT - Clinical Trial PT - Journal Article PT - Multicenter Study DEP - 20181129 PL - United States TA - Biomed Res Int JT - BioMed research international JID - 101600173 RN - 0 (Neoplasm Proteins) SB - IM MH - Adult MH - Aged MH - Female MH - Humans MH - Male MH - *Mass Spectrometry MH - Middle Aged MH - Neoplasm Proteins/*metabolism MH - Pancreatic Cyst/*metabolism/pathology MH - Pancreatic Neoplasms/*metabolism/pathology PMC - PMC6304507 EDAT- 2019/01/11 06:00 MHDA- 2019/04/26 06:00 PMCR- 2018/11/29 CRDT- 2019/01/11 06:00 PHST- 2018/04/27 00:00 [received] PHST- 2018/11/18 00:00 [accepted] PHST- 2019/01/11 06:00 [entrez] PHST- 2019/01/11 06:00 [pubmed] PHST- 2019/04/26 06:00 [medline] PHST- 2018/11/29 00:00 [pmc-release] AID - 10.1155/2018/7169595 [doi] PST - epublish SO - Biomed Res Int. 2018 Nov 29;2018:7169595. doi: 10.1155/2018/7169595. eCollection 2018.