PMID- 30627594
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2331-8325 (Print)
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 8
IP  - 24
DP  - 2018 Dec 20
TI  - Method for Studying the Effect of Gene Silencing on Bacterial Infection-induced 
      ERK1/2 Signaling in Bone-marrow Derived Macrophages.
LID - e3123 [pii]
LID - 10.21769/BioProtoc.3123 [doi]
AB  - Macrophages are highly phagocytic cells that utilize various pathogen recognition 
      receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs). 
      These PAMPs can be present within the microbe, such as bacterial CpG DNA, and are 
      recognized by Toll-like receptor 9 (TLR9), a PRR present on the endosomal 
      membrane of macrophages. PAMPs can also be present on the surface of microbes, 
      such as Lipopolysaccharide (LPS), which decorates the outer membrane of 
      gram-negative bacteria like Salmonella typhimurium and Escherichia coli. LPS is 
      recognized by TLR4 present on the plasma membrane of macrophages, and LPS-TLR4 
      association leads to activation of signaling cascades including MAPK 
      phosphorylation, which in turn promotes macrophage activation and microbial 
      killing. This protocol describes the method for studying the role of a gene of 
      interest in Extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) 
      signaling, induced by bacterial infection in primary bone-marrow derived 
      macrophages (BMDMs).
FAU - Kumar, Gaurav
AU  - Kumar G
AD  - Division of Cell Biology and Immunology, CSIR-Institute of Microbial Technology 
      (IMTECH), Chandigarh, India.
FAU - Arya, Subhash B
AU  - Arya SB
AD  - Division of Cell Biology and Immunology, CSIR-Institute of Microbial Technology 
      (IMTECH), Chandigarh, India.
FAU - Tuli, Amit
AU  - Tuli A
AD  - Division of Cell Biology and Immunology, CSIR-Institute of Microbial Technology 
      (IMTECH), Chandigarh, India.
LA  - eng
GR  - Wellcome Trust/United Kingdom
GR  - IA/I/14/2/501543/WTDBT_/DBT-Wellcome Trust India Alliance/India
GR  - R01 DK042748/DK/NIDDK NIH HHS/United States
GR  - R01 DK069703/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC6322648
MID - EMS81007
OTO - NOTNLM
OT  - Bone-marrow derived macrophages
OT  - ERK signaling
OT  - Infection
OT  - Salmonella
OT  - Western blotting
COIS- Competing interests The authors declare no competing financial interests.
EDAT- 2019/01/11 06:00
MHDA- 2019/01/11 06:01
PMCR- 2019/12/20
CRDT- 2019/01/11 06:00
PHST- 2019/01/11 06:00 [entrez]
PHST- 2019/01/11 06:00 [pubmed]
PHST- 2019/01/11 06:01 [medline]
PHST- 2019/12/20 00:00 [pmc-release]
AID - e3123 [pii]
AID - 3123 [pii]
AID - 10.21769/BioProtoc.3123 [doi]
PST - ppublish
SO  - Bio Protoc. 2018 Dec 20;8(24):e3123. doi: 10.21769/BioProtoc.3123.